A method for studying the molecular mechanism of plant stress tolerance using the grafting system of Salina thaliana and Arabidopsis
A system research, Arabidopsis technology, applied in the field of plant grafting and stress-resistant molecular biology, can solve the problems of incompatibility of distant grafting, difficulty in building the grafting system of Arabidopsis thaliana and salt mustard, etc. Conducive to normal germination and overcoming incompatibility of distant hybridization
Inactive Publication Date: 2020-06-26
SHANDONG NORMAL UNIV
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Problems solved by technology
However, Arabidopsis and Salina thaliana are two plants of the same family and different genus. The grafting between these two plants belongs to the distant grafting between genera. The incompatibility of distant grafting makes the grafting of Arabidopsis and Salina The construction of the system is extremely difficult
So far, there is no report on the study of the molecular mechanism of plant stress tolerance by constructing the grafting system of Salina thaliana and Arabidopsis
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[0058] Example 1: The method of studying the molecular mechanism of stress tolerance of plants using the grafting system of Salina thaliana and Arabidopsis
[0059] (1) Preparation of rootstock and scion material: at first, the salt mustard seeds full of grains are sterilized with 0.5% sodium hypochlorite and planted on the 1 / 2MS medium with agar powder concentration of 1.2%. Germinate vertically in long-day or short-day light incubators. When Salina thaliana germinates, use the same disinfection method to sterilize Arabidopsis seeds and plant them on 1 / 2MS medium with agar powder concentration of 1.2%. Stratify at 4°C or directly place them vertically in a long-day light incubator. Straight culture, 3-5 days after Arabidopsis seed germination, grafting of Arabidopsis / Salina thaliana (A / E), self-grafting of Arabidopsis / Arabidopsis (A / A), Self-grafting of (E / E). And the grafting of Saltina thaliana / Arabidopsis (E / A) is to wait for Arabidopsis seed to germinate for 5-7 days an...
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The invention discloses a method for studying a plant stress tolerance molecule mechanism by means of grafting systems of thellungiella halophila and arabidopsis thaliana. The method comprises the steps that the grafting systems of the thellungiella halophila and the arabidopsis thaliana are constructed; ICP-OES detection is conducted to observe changes of the salt stress tolerance before and after grafting; RNA is extracted for transcriptome expression profile detection to observe changes of genes before and after salt processing and the gene exchange conditions. According to the method, by means of mass experiment exploration, the thellungiella halophila-arabidopsis thaliana, thellungiella halophila-thellungiella halophila, arabidopsis thaliana-arabidopsis thaliana, arabidopsis thaliana-thellungiella halophila and arabidopsis thaliana-arabidopsis thaliana grafting systems are successfully constructed, and then the changes of the salt stress tolerance before and after grafting are observed by detecting the content of ion groups such as Na<+>, K<+> and Mg<2+> before and after salt processing of the grafting systems; the RNA is extracted for transcriptome expression profile detection to observe the changes of the genes before and after salt processing and the gene exchange conditions. The constructed grafting systems of the thellungiella halophila and the arabidopsis thaliana provide a new direction and through for studying the plant stress tolerance mechanism.
Description
technical field [0001] The invention relates to the technical field of plant grafting and stress tolerance molecular biology, in particular to a method for studying the molecular mechanism of plant stress tolerance by utilizing the grafting system of Salina thaliana and Arabidopsis thaliana. Background technique [0002] Grafting between plants is an ancient agronomic technique originated from our country. Today, in horticultural production, grafting is still a commonly used technique in the field of horticulture. As an agricultural production technology, it has been widely used in the cultivation and scientific research and development of agricultural, forestry and horticultural plants. Although grafting technology has a long history, a large number of researches began in the 1970s and 1980s. Especially in the past decade, with the rapid development of biotechnology, the research on grafting has gradually shifted from the study of traditional agronomic traits to the study o...
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IPC IPC(8): A01G2/30A01G2/35A01G31/00A01G7/06A01C1/08
CPCA01G2/30
Inventor 孙宇飞李燕孙伟李圆圆蔡宝珊谭倩刘书林游美红赵彦修张慧
Owner SHANDONG NORMAL UNIV