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Highly-stable urea reagent and detection method

A detection method and reagent technology, applied in the field of urea detection reagents, can solve problems such as poor reagent stability, and achieve the effects of good stability, improved stability, and enhanced stability

Inactive Publication Date: 2017-06-20
济南泰圣医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has the advantages of high specificity, rapidity, and accurate and precise results. It is currently the preferred chemical method for the determination of urea, but the stability of the reagents in this method is poor.

Method used

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  • Highly-stable urea reagent and detection method
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  • Highly-stable urea reagent and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Urea detection reagents, including reagent R1 and reagent R2:

[0056] 1) The composition of its R1 is:

[0057] Imidazole-HCL buffer ············································ 100mmol / L,

[0058] BSA ······················································· 1g / L,

[0059] ɑ-KG ······················································ 8mmol / L

[0060] sucrose ······················································ 5g / L,

[0061] Trehalose ···················································· 2g / L,

[0062] polyethylene glycol ·················································· 5g / L,

[0063] xanthan gum ···················································· 2g / L,

[0064] Mannitol ···················································· 4g / L,

[0065] maltose ···················································· 5g / L,

[0066] GLDH ······················································ 2KU / L,

[0067] Triton X-100· ············································· 2mL / L...

Embodiment 2

[0089] Correlation experiment: using the formula in Example 1 to prepare reagents, and conducting a control test with the urea kit of a company approved by the State Food and Drug Administration, which is common in the market, and testing 20 clinical serum samples at the same time, the test results are shown in Table 2 . And obtained the correlation curve of the two reagents (such as figure 1 Shown), the test results show that the correlation coefficient of the two kits is 0.9997, indicating that there is a great correlation between the two.

[0090] Table 2 Example 1 reagent and the common and approved urea assay kit comparative detection results in the market

[0091]

Embodiment 3

[0093] Contrast test of the stability of the validity period of the reagent: For the reagent in Example 1, 13 groups were evenly distributed, and the reagent volume of each group was 15 mL for R1 and 5 mL for R2; A urea kit from a certain company was used as a control. Place it in a refrigerator at 2-8°C, take out a set of reagents on the same day every month to test the urine quality control product (target value is 7.31mmol / L), the test results are as follows: figure 2 As shown, the reagent of Example 1 is more stable than the common urea assay kits in the market under the storage condition of 2-8°C.

[0094] Through verification, this reagent has a good correlation with similar detection reagents, and the results of clinical test samples are consistent, which can meet the application requirements of the market for products. It is a more stable and good urea detection reagent.

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Abstract

The invention relates to the technical field of urea detection, and especially relates to a urea detection reagent. A reagent R1 contains an imidazole buffer solution, BSA, alpha-oxpentanedioic acid, sucrose, trehalose, GLDH, polyethylene glycol, dodecyltrimethylammonium chloride, lauryl imidazolinium betaine and an antiseptic; and a reagent R2 contains a Tris buffer solution, the BSA, urease, sucrose, trehalose, NADH and the antiseptic. The imidazole buffer solution is adopted, and the BSA, sucrose, trehalose, polyethylene glycol, xanthan gum and mannitol which are used as a stabilizer are added, so the stability of the reagent is greatly enhanced; and dodecyltrimethylammonium chloride and the lauryl imidazolinium betaine which are used as novel surfactants substantially improve the detection performance and enhances the stability of the reagent.

Description

technical field [0001] The invention relates to the technical field of urea detection, in particular to a urea detection reagent with good stability and high sensitivity, and also relates to a detection method using the detection reagent. Background technique [0002] Urea is the end product of protein catabolism in mammals. Synthesized in the liver through the ornithine cycle, mainly excreted by the kidneys, and can be freely filtered out from the glomeruli. Because urea has a small molecular weight and is easy to dissolve, it has a great diffusion force, so the concentration of urea in cerebrospinal fluid, serous cavity effusion, saliva and sweat is basically the same. Blood urea concentration is mainly influenced by renal function and protein intake and catabolism. According to relevant literature reports: when the content of urea in the human body exceeds its own absorption capacity, it will cause harm to the liver, kidneys, and alveoli of the human body. The increase...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/58C12Q1/34C12Q1/32
CPCC12Q1/32C12Q1/34C12Q1/58
Inventor 甘宜梧罗维晓王进
Owner 济南泰圣医疗器械有限公司
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