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Beauveria bassiana wettable powder and application thereof

A wettable powder, Beauveria bassiana spore powder technology, applied in the application, insecticide, biocide and other directions, can solve the problems of slow research progress, easy to lose vitality, adverse effects on spore activity, etc., and achieve good environmental compatibility , human and animal safety, the selection of additives and the effect of reasonable ratio

Active Publication Date: 2017-06-23
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, domestic research progress on Beauveria bassiana wettable powder is relatively slow. The main reason is that Beauveria bassiana wettable powder tends to lose its vitality during storage, and some additives will adversely affect the activity of spores.

Method used

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  • Beauveria bassiana wettable powder and application thereof
  • Beauveria bassiana wettable powder and application thereof
  • Beauveria bassiana wettable powder and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0047] As an embodiment of the present invention, in parts by weight, the wettable powder of Beauveria bassiana comprises 50 parts of Beauveria bassiana spore powder, 18 parts of diatomaceous earth, 4 parts of pull-open powder BX, and 3 parts of NP-10 , AEO-35 parts, activated carbon 15 parts and potassium dihydrogen phosphate 5 parts.

[0048] In the present invention, the Beauveria bassiana is a strain of Beauveria bassiana.

[0049]As an embodiment of the present invention, the Beauveria bassiana is Beauveria bassiana strain EHM-068, which is classified as Beauveria Bassiana and has been preserved in China Microorganisms on March 30, 2016. The General Microbiology Center of the Culture Collection Management Committee, the preservation address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCCNo.12110. The nucleotide sequence of Beauveria bassiana strain EHM-068 is shown in SEQ ID NO:1.

[0050] In the present invention, t...

experiment example 1

[0051] Experimental Example 1 Screening of Carriers

[0052] Different carriers: diatomaceous earth, attapulgite, kaolin and silica

[0053] Test method: Conidia powder of Beauveria bassiana coccidioides and four different test carriers of diatomite, attapulgite, kaolin and white carbon black were uniformly mixed according to the ratio of 1:1 in parts by weight, and placed in In a low-temperature freezer, store in the dark at a temperature of (3±1)°C and a relative humidity of 5%. Take quantitative mixed powder every month and put it in the spore germination solution, and culture it with shaking at (25±1)°C and 120r / min for 24 hours. The germination rate of the spores was then measured, and the pure spore powder of Beauveria bassiana stored under the same conditions was used as a control, and 5 samples were measured each time for a total of 5 months.

[0054] Four different carriers were evenly mixed with Beauveria bassiana spores and stored for 5 months, and the spore germin...

experiment example 2

[0058] Experimental example 2 Screening of wetting agent

[0059] Different wetting agents: Morwet EFW, MF-5 (methylnaphthalenesulfonate formaldehyde condensate), K12 (sodium lauryl sulfate) and pull-off powder BX (sodium diisobutylnaphthalenesulfonate)

[0060] Test method: Take 100mL±1mL of standard hard water, pour it into a 250mL beaker, place the beaker in a constant temperature water bath at 25±1°C, and make the liquid level flush with the water bath level. When the hard water reaches 25±1°C, weigh 5g±0.1g of the sample (the wetting agent and Beauveria bassiana are uniformly mixed at a ratio of 1:10), place it on a watch glass, and put all the samples from the mouth of the beaker. Pour it evenly on the liquid surface of the beaker in a flat position at one time, but do not disturb the liquid surface too much. When adding the sample, immediately use a stopwatch to count until the sample is completely wet, and record the wetting time. This was repeated 5 times, and the av...

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Abstract

The invention relates to the technical field of microbial pesticides, in particular to beauveria bassiana wettable powder and application thereof. The beauveria bassiana wettable powder provided by the invention comprises beauveria bassiana conidial powder, a carrier, a wetting agent, a dispersing agent, an emulsifying agent, an absorbing agent and a regulating agent. The beauveriaceae wettable powder is reasonable in aid and proportion selection, good in quality indexes, good in activity, good in effect on prevention and treatment of ostrinia nubilalis and chilo suppressalis walker, convenient to use, long in storage time, low in cost, safe to people and animals, and good in environmental compatibility.

Description

technical field [0001] The invention relates to the technical field of microbial pesticides, in particular to a Beauveria bassiana wettable powder and its application. Background technique [0002] Due to the long-term and large-scale use of chemical pesticides, the resistance of pests has increased, and at the same time it will kill the natural enemies of pests, resulting in frequent disasters of crop pests. The use of biological insecticides based on entomopathogenic microorganisms to replace chemical pesticides has become an important means of integrated pest control technology. [0003] Corn borer (Ostrinia furnacalis Guenée), belongs to Lepidoptera, Otriidae, also known as corn borer, mainly damages corn, sorghum, millet, etc., and can also damage cotton, sugarcane, hemp, sunflower, rice, sugar beet, sugarcane beans and other crops, the corn borer is widely distributed, serious damage, strong ecological adaptability, and belongs to the worldwide pests. The corn borer ...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01N25/14A01P7/04
CPCA01N25/14A01N63/30A01N2300/00
Inventor 田志来朱晓敏张强
Owner JILIN ACAD OF AGRI SCI
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