Combination of icariin and hyaluronic acid and its preparation method and application
A technology of icariin and hyaluronic acid, which is applied in the field of medicine, can solve problems such as aggravating articular cartilage degeneration and failing to prevent disease progression, so as to relieve pain, improve daily activities and joint range of motion, and reduce adverse reactions Effect
Active Publication Date: 2019-07-23
张君涛
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Problems solved by technology
Most of the previous treatment methods can only relieve symptoms in the short term, but cannot prevent the progression of the disease, and will aggravate the degeneration of articular cartilage
Method used
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Embodiment Construction
[0040] The conjugate of icariin and hyaluronic acid is prepared by the following steps:
[0041] Step 1: Amino protection of glycine:
[0042] 2mmol glycine (Gly) was dissolved in 20mL NaHCO3 solution (mass fraction is 1.68%), stirred at room temperature for 1-2h, at the same time, 0.45g di-tert-butyl dicarbonate was dissolved in 2mL dioxane, and then, Under the condition of constant stirring in the ice bath, slowly drop the di-tert-butyl dicarbonate solution into the Gly solution, control the dropping speed and finish the dropping within 10 minutes, then react at room temperature for 20 hours;
[0043]Adjust the pH of the solution after the above reaction to neutral with 0.01M HCl, remove the solvent with a rotary evaporator, then dissolve the remaining solid with dichloromethane (DCM), and remove the insoluble matter (NaCl); the reaction dissolved in DCM The product was added dropwise to 10 times the volume of cold ether to precipitate, and the solid product was collected b...
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The invention relates to an icariin and hyaluronic acid composition, a method for preparing the same and application of the icariin and hyaluronic acid composition. The icariin and hyaluronic acid composition, the method and the application have the advantages that the icariin and hyaluronic acid composition can be used for preparing medicines for treating cartilage diseases and can be particularly used for preparing injection for joint cavities, accordingly, the shortcoming of limitation of single hyaluronic acid treatment can be overcome, the application range can be expanded, and adverse reaction can be reduced; effects of covering and protecting the surfaces of cartilage can be enhanced by the aid of pharmacological effects of icariin on cartilage cells; the icariin and hyaluronic acid composition can act on synovial cells, synthesis of autologous macromolecular hyaluronic acid can be promoted, and accordingly the purposes of nourishing the cartilage, lubricating the joint cavities, relieving pain, improving the daily activity ranges and the joint activity ranges of patients and the like can be achieved; the icariin and hyaluronic acid composition can act on the cartilage cells, accordingly, the viability of the cartilage cells can be enhanced, secretion of active substances such as type II collagen of the cartilage cells and mucopolysaccharide can be promoted, and nourishing, lubricating, regenerating and functional reconstruction effects and the like can be jointly realized; knowledge on traditional Chinese medicine injection can be changed, the adverse reaction can be reduced to a great extent, curative effects of hyaluronic acid and the traditional Chinese medicine injection can be improved, and the application ranges of the hyaluronic acid and the Chinese medicine injection can be expanded.
Description
technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a conjugate of icariin and hyaluronic acid, a preparation method and application thereof. Background technique [0002] Hyaluronic acid is a disaccharide unit composed of D-glucuronic acid and N-acetylglucosamine Hyaluronic acid, also known as uronic acid, the basic structure is composed of two disaccharide units D-glucuronic acid and N-acetylglucosamine composed of large polysaccharides. Unlike other mucopolysaccharides, it does not contain sulfur and is an acidic mucopolysaccharide. With its unique molecular structure and physical and chemical properties, hyaluronic acid shows a variety of important physiological functions in the body, such as lubricating joints, regulating the permeability of blood vessel walls, regulating protein, water and electrolyte diffusion and operation, and promoting wound healing. [0003] Sodium hyaluronate, namely sodium hyaluronate, ...
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IPC IPC(8): C08B37/08A61K31/728A61P19/08A61P19/02
CPCC08B37/0072
Inventor 张君涛赵芳李妍张栋林
Owner 张君涛