A method for liquid fermentation of Cordyceps militaris strain

Abstract

The invention provides a method for liquid fermentation of a Cordyceps militaris strain, comprising the following steps: inoculating the Cordyceps militaris strain in a liquid fermentation medium, cultivating on a shaking table, controlling the temperature between 19-21°C, and controlling the rotating speed of the shaking table at 200 ‑220rad / min, dark culture 8‑14d; liquid fermentation medium is prepared from the fruit or root of Araliaceae plants as raw materials. In the method for liquid fermentation of the Cordyceps militaris strain of the present invention, the fermentation substrate mainly adopts the fruit or root of the Araliaceae plant as a raw material, which is relatively easy to obtain and does not need to add other ingredients, and the preparation process is simple and convenient, economical and environmentally friendly. The product fermented by a specific medium not only increases the content of cordycepin, but also makes the product contain a large amount of rare saponins, and provides a more novel operation route for the liquid fermentation of the Cordyceps militaris strain, which is worthy of vigorous promotion and application. It also fills in the relevant technical gaps.

Description

technical field [0001] The invention relates to the field of fermentation of Cordyceps militaris strains, in particular to a method for liquid fermentation of Cordyceps militaris strains. Background technique [0002] Cordyceps militaris is Ascomycotina, Ergotaceae, Ergotaceae, and Cordyceps genus. It is a complex composed of two parts: the subterranean base and the fruiting body part above the ground. In the worm body, the larva absorbs nutrients, and the larvae are filled with hyphae and die. In the summer and autumn of the second year, grass bodies grow from the larvae, and wild Cordyceps militaris can be collected around mid-September. Cordycepin contained in Cordyceps militaris has a variety of physiological activities, such as anti-tumor, anti-virus, and immune-enhancing effects. [0003] In the prior art, chemical synthesis and biosynthesis are generally used to obtain cordycepin. The production cost of chemical synthesis is relatively high, the synthesis process i...

AI Technical Summary

Problems solved by technology

Due to the advantages of liquid fermentation over solid fermentation in terms of fermentation scale, cell growth rate, growth density and controllability, the extraction of cordycepin by liquid fermentation of Cordyceps militaris is the main application method for preparing cordycepin nowadays, and the fermentation method is relatively mature. However, the content of cordycepin extracted by the current fermentation method is maintained at a stable level, and it is difficult to make new breakthroughs under the limitations of the existing technological conditions. The preparation method of the fermentation medium used is relatively cumbersome and difficult to operate. It also increases the workload of extracting cordycepin, consumes manpower and material resources, and is not economical and environmentally friendly.