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Application of biological active peptide of sea cucumbers to preparation of antioxidation aging-delaying health food and cosmetics

A technology of sea cucumber polypeptide and sea cucumber, applied in the field of marine biology

Inactive Publication Date: 2017-07-04
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the biological activity of sea cucumber peptides is significant, there are few reports on the material basis of its efficacy

Method used

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  • Application of biological active peptide of sea cucumbers to preparation of antioxidation aging-delaying health food and cosmetics
  • Application of biological active peptide of sea cucumbers to preparation of antioxidation aging-delaying health food and cosmetics
  • Application of biological active peptide of sea cucumbers to preparation of antioxidation aging-delaying health food and cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Fresh sea cucumbers were dissected, viscera removed, body wall washed 3-4 times with tap water and distilled water, then homogenized sea cucumber body wall tissue material, freeze-dried, weighed 5g into a 250mL beaker and added 80mL water to infiltrate (1g dry powder mixed with 20mL of first-class water, to be supplemented with the volume of enzyme solution later), can be stirred properly with a glass rod, then preheated to 45°C in a water bath, adjusted the pH value to 8.0 with NaOH solution, and the amount of 3000U trypsin per gram of substrate , add trypsin with an enzyme activity of 2500U / mg, hydrolyze at 37-55°C for 2-10 hours at a constant temperature, and inactivate by boiling at 100°C; then, when the temperature drops to about 55°C, adjust the pH to 0.1M HCl solution 5.5, add 3000U papain per gram of substrate, add papain with an enzyme activity of 6000U / mg, hydrolyze at 50-80°C for 2-10 hours at a constant temperature, and inactivate by boiling at 100°C to obtai...

Embodiment 2

[0069] Use first-grade water to make sea cucumber polypeptide freeze-dried powder into a solution with a concentration of 10mg / mL, and use an ultrafiltration centrifuge tube with a molecular weight cut-off of 3kDa to centrifuge at 6000-8000rpm to collect polypeptide components with a molecular weight less than 3kDa (MW<3kDa), The corresponding polypeptide samples were obtained after lyophilization.

Embodiment 3

[0071] The sea cucumber polypeptide components with a molecular weight less than 3kDa were separated and purified on a Sephadex chromatography column, with a sample volume of 2mL and a concentration of 20mg / mL for injection into the needle, using water as the mobile phase, The speed of 1mL / min was eluted, and the ultraviolet detector detected the characteristic peak of the polypeptide component at 280nm. The eluate of each main component peak was collected, and the polypeptide components corresponding to the five peaks were obtained and named as F1, F2, F3, F4 and F5 through a vacuum freeze dryer respectively, such as figure 1 shown.

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Abstract

The invention specifically relates to sea cucumber polypeptide and a preparation method and application thereof, belonging to the field of marine biotechnology. The sea cucumber polypeptide has an amino acid sequence selected from amino acid sequences as shown in SEQ ID No. 1 to SEQ ID No. 13. The F2 component of the sea cucumber polypeptide contains amino acid sequences as shown in SEQ ID No. 1 to SEQ ID No. 11; and the F3 component of the sea cucumber polypeptide contains amino acid sequences as shown in SEQ ID No. 12 to SEQ ID No. 13. The preparation method provided by the invention can make full use of the abundant sea cucumber resources for preparation of the F2 and F3 components of the sea cucumber polypeptide. Experimental results show that the sea cucumber polypeptide with the amino acid sequence selected from amino acid sequences as shown in SEQ ID No. 1 to SEQ ID No. 13 and the F2 and F3 components of the sea cucumber polypeptide all have antioxidation and anti-ageing effect, can be used for preparation of antioxidation and anti-ageing medicines or health products, and have good economic and social effects.

Description

technical field [0001] The invention belongs to the technical field of marine organisms, and in particular relates to sea cucumber polypeptides and their preparation methods and applications. Background technique [0002] Traditional medicine has a long history of research on longevity and neuroprotection, and abundant marine biological resources provide a huge resource pool, including peptides, proteins, polysaccharides, alkaloids, terpenes, macrocycles Tens of thousands of new compounds such as polyesters have been discovered. Among them, peptides are the largest class of compounds among marine bioactive substances, with tens of thousands of species, including marine peptide toxins and marine bioactive peptides. [0003] Due to the specific environment in which marine organisms live, the structure of marine biological peptides is very different from that of terrestrial animal and plant peptides (glycopeptides). In addition to the characteristics of non-immunogenicity, it...

Claims

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Application Information

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IPC IPC(8): C07K7/06C07K7/08C12P21/06C12P21/02C07K1/36C07K1/34C07K1/16A61K38/08A61K38/10A61P39/06A61P39/00A23L33/18A61K8/64A61Q19/08
CPCA61Q19/08A61K8/64C12P21/02C12P21/06C07K7/06C07K7/08A61K38/00
Inventor 梁明马忠华郭晓蕾黄泽波秦垂新毛新亮贾伟章
Owner INFINITUS (CHINA) CO LTD
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