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Mastra veneriformis polypeptide with in-vivo anti-oxidation and neuroprotection functions, and preparation method and application thereof

A neuroprotective and antioxidative technology, which can be applied in the preparation methods of peptides, chemical instruments and methods, and medical preparations containing active ingredients, etc. role, good economic and social effects, effects with a wide range of applications

Inactive Publication Date: 2017-07-04
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the domestic and foreign studies on white clams focus on its growth environment, breeding, food processing, and species identification. However, there are few research reports on the biological activity of white clam polypeptides, which have broad development prospects.

Method used

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  • Mastra veneriformis polypeptide with in-vivo anti-oxidation and neuroprotection functions, and preparation method and application thereof
  • Mastra veneriformis polypeptide with in-vivo anti-oxidation and neuroprotection functions, and preparation method and application thereof
  • Mastra veneriformis polypeptide with in-vivo anti-oxidation and neuroprotection functions, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1, the preparation of white clam polypeptide

[0042] Fresh white clams were shelled and meat was removed, washed with water for 3 to 4 times, and after washing the sediment, the soft tissues were taken and mixed with 2 times the volume of third-grade water for homogenization, and the minced meat obtained was freeze-dried and weighed. Weigh a certain amount of white clam protein, add 20 times the volume of tertiary water to redissolve, add 1M HCl solution to adjust the pH value of the system to 6.0 at a water bath temperature of 60°C, and then add the enzyme mother solution (30000U / mL ) and substrate at a volume ratio of 1:400, papain was added, and after enzymatic hydrolysis for 6 hours, the enzymatic activity was inactivated by boiling at 100°C for 10 minutes.

[0043] The above clam protein enzymatic hydrolyzate was formulated into a 20 mg / mL solution, first filtered through a 0.45 μm water-based microporous membrane, and the collected filtrate was interce...

Embodiment 2

[0044] Example 2, Anti-paraquat-induced oxidative stress experiment

[0045] The synchronized wild-type C. elegans (N2) was cultured to the L4 stage, and 5-FUdR with a final concentration of 75 μg / mL was added to inhibit its oviposition. After growing to the early adult stage, 10-15 C. elegans / well coli NA22 as food, each group was fed with 0.5mg / mL, 1mg / mL and 2mg / mL of the white clam polypeptide prepared in Example 1, and the control group was added. The volume of S Medium was cultured at 20°C for 24 hours, then paraquat with a final concentration of 80mM was added to establish the oxidative stress model, and the survival of Caenorhabditis elegans at each concentration was counted every 12 hours until all of them died. During the statistical process, the nematodes whose bodies were stiff and did not respond to slight vibrations were recorded as dead. The sample size of nematodes in each group was 100-150, and the results were analyzed by Kaplan-Meier method. The result is ...

Embodiment 3

[0047] Embodiment 3, the detection of reactive oxygen species (ROS) level

[0048] The synchronized N2 C. elegans was cultured to the L4 stage, and the amount of 4000 / well was added to 24 wells with 5-FUdR, E.coli NA22, 1 mg / mL and 2 mg / mL clam polypeptides prepared in Example 1 In the culture plate, the final volume of each well is 1mL. After continuing to cultivate for 48h, collect nematodes from each group and homogenize them in an ice bath. Centrifuge the homogenate at 4°C and 10,000g for 5 minutes, collect the supernatant, and add the final concentration After incubating with 50 μM DCFH-DA probe solution for 2 h, the fluorescence intensity was detected with a fluorescent microplate reader at an excitation wavelength of 485 nm and an emission wavelength of 538 nm. The result is as figure 2 shown.

[0049] The results showed that after feeding with 1 mg / mL and 2 mg / mL of clam polypeptide prepared in Example 1, the ROS level in C. elegans decreased to a certain extent com...

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Abstract

The invention specifically relates to a Mastra veneriformis polypeptide, and a preparation method and application thereof, belonging to the field of marine biotechnology. The preparation method for the Mastra veneriformis polypeptide comprises the following steps: adding water into the soft body of Mastra veneriformis, carrying out homogenization, then adding papain for hydrolysis, carrying out ultrafiltration and then collecting components with molecular weight less than 10 kDa. Experimental results show that the Mastra veneriformis polypeptide prepared by the invention has good anti-oxidation and neuroprotective effect, can be used for preparation of drugs or health products with anti-oxidation and neuroprotective effect, and has good economical and social effect.

Description

technical field [0001] The invention belongs to the technical field of marine biology, and in particular relates to a white clam polypeptide and its preparation method and application. Background technique [0002] With the aging of the social population, the incidence of aging-related neurodegenerative diseases is also increasing year by year worldwide, and the number of patients is increasing year by year. The clinical manifestations are mainly divided into two types: motor dysfunction and memory and cognitive function. obstacle. Histopathological studies have shown that neurons undergo slow and progressive apoptosis, and most of them have abnormal protein aggregation and inclusion bodies in neurons, suggesting that they may have similar pathogenesis. Therefore, actively searching for bioactive substances that can relieve apoptosis and aging and inhibit the abnormal aggregation of neurotoxic proteins will provide a certain material basis for the prevention and treatment o...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34C07K2/00A61K38/01A61P39/06A61P39/00A23L33/18
CPCA61K38/012C12P21/06C07K2/00
Inventor 梁明郭晓蕾张婷黄泽波汪强强黄韵璇
Owner INFINITUS (CHINA) CO LTD
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