Application of phosphorylated chitosan as immuno-adjuvant in vaccine therapy

A technology of phosphorylated chitosan and immune adjuvant, which is applied to medical preparations containing active ingredients, antibody medical ingredients, pharmaceutical formulations, etc., can solve problems such as enhancing specific immune responses, and achieve antigen-specific immune responses , low cost, good water solubility

Inactive Publication Date: 2017-07-14
JINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Application of phosphorylated chitosan as immuno-adjuvant in vaccine therapy
  • Application of phosphorylated chitosan as immuno-adjuvant in vaccine therapy
  • Application of phosphorylated chitosan as immuno-adjuvant in vaccine therapy

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Embodiment 1

[0034] One, the preparation of phosphorylated chitosan:

[0035] 10g of chitosan (3000cps) was dissolved in 500mL of 1% (v / v) acetic acid, and the temperature was raised to 70°C. Dissolve 10g of phosphorous acid in 20mL of water and add it to the reaction system at one time. After mixing evenly, add 13 mL of 37% to 40% formaldehyde solution and stir for 3 hours. After the reaction, the reaction solution was added into absolute ethanol for separation and precipitation. Repeated washing and purification with ethanol twice, dialysis and freeze-drying to obtain water-soluble phosphorylated chitosan.

[0036] Two, the preparation of 6 kinds of different formula vaccines:

[0037] With ovalbumin OVA as the antigen, vaccines of different formulations were prepared according to the following table, which were normal saline (blank control group), OVA / normal saline (negative control group), 1mg / mL PCS / OVA, 10mg / mL PCS / OVA, 30mg / mLPCS / OVA and Freund's adjuvant (positive control grou...

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Abstract

The invention discloses an application of phosphorylated chitosan as an immuno-adjuvant in vaccine therapy and belongs to the field of biomaterials and immuno-treatment. In the invention, for the first time, the phosphorylated chitosan serves as the immuno-adjuvant; compared with conventional immuno-adjuvants, the phosphorylated chitosan is easy to synthesize and is low in cost, and has excellent biodegradability and biocompatibility and has no damage on both organisms and environment. The phosphorylated chitosan is good in water-solubility and can be applied in in-vivo environment well without adverse toxic and side effects. The phosphorylated chitosan, as an immuno-adjuvant, can significantly induce higher-level antigen-specificity IgG antibody titer, promote immuno-reaction of IFN-gamma/IL-4 cell factor and CD4<+>/CD8<+> T lymphocytes and activate dendritic cells better, thereby enhancing immunogenicity of a vaccine and effectively improving antigen-specific immune response of organisms, so that the phosphorylated chitosan has important application value in the field of vaccine therapy.

Description

technical field [0001] The invention belongs to the field of biological materials and immunotherapy, and in particular relates to the application of phosphorylated chitosan as an immune adjuvant in vaccine therapy. Background technique [0002] Chitosan has been widely studied because of its good biocompatibility, bioactivity, biodegradability, and bioadhesion. Studies have shown that chitosan as an antigen delivery carrier can significantly improve the immune activity of subunit vaccines. However, chitosan is only soluble in dilute acid conditions, which limits its application in biomedical fields. In order to overcome this shortcoming, there have been many reports that researchers have modified chitosan for water solubility and used it to prepare immune adjuvants. At present, more research at home and abroad is to quaternize chitosan and use it to improve the IgG antibody titer and hemagglutination inhibitory antibody level of subunit vaccines (Amidi, M., et al., N-Trime...

Claims

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Application Information

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IPC IPC(8): A61K39/39
CPCA61K39/39A61K2039/55583
Inventor 刘宗华薛巍魏建业
Owner JINAN UNIVERSITY
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