Production method of milbemycin

Abstract

The invention provides a production method of milbemycin. The production method comprises the following steps: (S1) dissolving a milbemycin extract by virtue of a first solvent, so as to obtain a first milbemycin dissolving solution; (S2) adding a second solvent into the first milbemycin dissolving solution obtained in the step (S1), stirring to crystallize, so as to obtain coarse crystallization powder; (S3) dissolving the coarse crystallization powder obtained in the step (S2) with the first solvent, so as to obtain a second milbemycin dissolving solution; and (S4) adding the second solvent into the second milbemycin dissolving solution obtained in the step (S3), stirring to recrystallize, so as to obtain milbemycin recrystallization powder, wherein the first solvent is any one of ethanol, acetone, ethyl acetate or isopropyl-ketone, and the second solvent is any one of n-hexane, n-heptane, n-octane, n-nonane, n-decane, n-undecane or n-dodecane. The production method provided by the invention has the advantages that the process is simple, the production cycle is short, the recycling amounts of the solvents are low, and the prepared milbemycin has high purity.

Description

technical field [0001] The invention relates to the field of biopharmaceuticals, in particular to a production method of milbemycin. Background technique [0002] Milbemycin is a mixture with hexadecanolide structure isolated from Milbemycin fermentation broth. As a new biological pesticide, milbemycin is broad-spectrum, highly efficient and low-toxic, and is one of the most active insecticides; at the same time, milbemycin is also the direct raw material for the synthesis of milbemycin, which is a The novel synthetic macrolide anthelmintic drug has good effects on controlling and preventing most parasitic diseases. [0003] At present, the production method of milbemycin is to filter out the bacteria residue from the fermentation broth, and then use acetone or ethanol to extract the bacteria residue to obtain a milbemycin extract; Dilute the diluted milbemycin extract (column solution) with resin, and elute with ethanol-water or acetone-water to obtain an eluate; concentr...

AI Technical Summary

Problems solved by technology

The resin separation process of this production method generally takes 3 days, and the amount of solvent used is relatively large. It takes about 15 m to separate 10 kg of milbemycin in production. 3 That is, 15000L of acetone or ethanol, the recovery of the solvent is relatively large, and the energy consumption is high. At the same time, the purity of the milbemycin obtained by resin separation is 70-80%.

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