Method for extracting fish collagen

A technology of fish collagen and extraction method, which is applied in the field of fish collagen extraction, can solve the problems of long operation time, pollution, low fat content, etc., and achieve the effect of less fat content, good water solubility and high purity

Active Publication Date: 2017-07-21
GUANGDONG TIMES FOOD & LIFE HEALTH RES CO LTD
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  • Abstract
  • Description
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Problems solved by technology

[0007] The purpose of the present invention is the problem of long operating time and serious pollution caused by the need to use more organic solvents in the lipid and protein separation process of fish collagen; the present invention adopts a method that does not use organic solvents The fish collagen extraction process realizes the removal and separation of fat. The main technical concept is: firstly, through the grinding an

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  • Method for extracting fish collagen
  • Method for extracting fish collagen
  • Method for extracting fish collagen

Examples

Experimental program
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Example Embodiment

[0029] Example 1

[0030] Step 1, tissue crushing: after thawing the skin of crucian carp, add water to make the weight ratio of skin to water at 10:0.2, crush and grind the mixture, and add ultrasonic action while crushing and grinding to obtain a slurry ;

[0031] The second step is to remove salt-soluble impurities: mix the slurry with 4wt% NaCl solution, the weight ratio of the slurry to the NaCl solution is 1:5, stir evenly, and then soak for 10 hours. After centrifugation, remove the supernatant to obtain first precipitation;

[0032]The third step, remove lipids and alkali-soluble impurities: mix the first precipitate with 2wt% NaOH solution, the weight ratio of the first precipitate and the alkali solution is 1:7, stir evenly, soak for 20h, and then go through centrifugation to remove supernatant to obtain the second precipitate;

[0033] Step 4, remove acid-soluble impurities: mix the second precipitate with 5wt% citric acid solution, the weight ratio of the second...

Example Embodiment

[0038] Example 2

[0039] Step 1, tissue crushing: after thawing the skin of crucian carp, add water to make the weight ratio of the skin to water at 10: 1, crush and grind the mixture, and add ultrasonic action while crushing and grinding to obtain a slurry ;

[0040] The second step, remove salt-soluble impurities: mix the slurry with 10wt% NaCl solution, the weight ratio of the slurry to the NaCl solution is 1: 10, stir evenly, and then soak for 30 hours. After centrifugation, remove the supernatant to obtain first precipitation;

[0041] The 3rd step, remove lipids and alkali-soluble impurities: mix the first precipitation with 5wt% NaOH solution, the weight ratio of the first precipitation and the alkali solution is 1: 12, stir evenly, soak, soak for 35h, and then undergo centrifugal treatment to remove supernatant to obtain the second precipitate;

[0042] The 4th step, remove acid-soluble impurities: mix the second precipitation with 12wt% citric acid solution, the w...

Example Embodiment

[0047] Example 3

[0048] Step 1, tissue crushing: after thawing the skin of crucian carp, add water to make the weight ratio of skin to water at 10:0.6, crush and grind the mixture, and add ultrasonic action while crushing and grinding to obtain a slurry ;

[0049] The second step is to remove salt-soluble impurities: mix the slurry with 6wt% NaCl solution, the weight ratio of the slurry to the NaCl solution is 1:7, stir evenly, and then soak for 20 hours. After centrifugation, remove the supernatant to obtain first precipitation;

[0050] Step 3, remove lipids and alkali-soluble impurities: mix the first precipitate with 4wt% NaOH solution, the weight ratio of the first precipitate and the alkali solution is 1:11, stir evenly, soak for 26 hours, and then go through centrifugation to remove supernatant to obtain the second precipitate;

[0051] Step 4, remove acid-soluble impurities: mix the second precipitate with 5-12wt% citric acid solution, the weight ratio of the seco...

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Abstract

The invention relates to a method for extracting fish collagen, and belongs to the technical field of natural protein extraction. Through adoption of a fish collagen extracting process in which an organic solvent is not used, removal and separation of fat are achieved. The invention adopts the technical concept as follows: firstly, through grinding and homogenizing under an ultrasonic action, tissue cells of fish skin are completely broken, and during subsequent alkali treatment and enzymatic treatment, fat and protein can be more removed, so that a crude extract with low fat content can be obtained; then, a crude extract solution is enabled to pass through a resin bed obtained after lipophilic modification, so that the purpose of adsorbing low-content lipids is achieved. The prepared fish collagen has the advantages of high purity, low fat content and good water solubility.

Description

technical field [0001] The invention relates to a method for extracting fish collagen, belonging to the technical field of natural protein extraction. Background technique [0002] Collagen is a white, opaque, unbranched, fibrous protein that plays the role of binding tissue in animal cells. Collagen is rich in 18 kinds of amino acids except tryptophan and cysteine, among which there are 7 kinds of amino acids necessary to maintain human growth. Glycine in collagen accounts for 30%, proline and hydroxyproline account for about 25%, which is the highest content in various proteins, and the content of alanine and glutamic acid is also relatively high. At the same time, it also contains hydroxyproline and pyroglutamic acid, which are rare in general proteins, and hydroxylysine, which hardly exists in other proteins. Therefore, collagen is very nutritious. Fish collagen is mainly extracted from tilapia, cod fresh skin and fish scales through bio-enzyme directional shearing te...

Claims

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Application Information

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IPC IPC(8): C07K14/78C07K1/22
CPCC07K14/78
Inventor 张军志
Owner GUANGDONG TIMES FOOD & LIFE HEALTH RES CO LTD
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