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On-line collection and on-line automatic detection method of bacteria and viruses in air

A technology of automatic detection and automatic detection device, which is applied in the field of microbial detection and can solve problems such as inability to warn or monitor bacteria or viruses.

Active Publication Date: 2017-07-21
SHANGHAI DEJU BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide a method for online collection and online automatic detection of bacteria or viruses in the air, so as to solve the problem that the bacteria or viruses in the air cannot be pre-warned or monitored, so as to realize rapid and accurate detection of bacteria or viruses in the air. On-site inspection

Method used

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  • On-line collection and on-line automatic detection method of bacteria and viruses in air
  • On-line collection and on-line automatic detection method of bacteria and viruses in air
  • On-line collection and on-line automatic detection method of bacteria and viruses in air

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Experimental program
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Effect test

Embodiment 1

[0080] Add 1ml of commercially available DNA (RNA) adsorption particles to the liquid in the liquid collection area, adjust the pH of the solution to 6-8 with 0.01mol / L NaOH and HCl respectively, and carry out the collection experiment of influenza virus in the air. After the murine lung-adapted influenza A virus is rejuvenated by chicken embryos, use 0.1mol / L phosphate solution to prepare a solution of 100,000 viruses / ml, and then add it to a volume of about 50m 3 In a confined space, the virus is sprayed in the air with an air distributor, and the specific number of virus particles is calculated according to the reduction of the liquid in the sprayer (the virus particles in the air are about 1000 / m 3 ). The volume of liquid in the liquid collection area is 15ml, the ratio of height to diameter is 3:1, and different methods are used to collect 0.1m 3 The influenza virus was sampled and detected in two batches, each with three batches, and the experimental results (Table 1). ...

Embodiment 2

[0086]Add 1ml of commercially available DNA (RNA) adsorption particles to the liquid in the liquid collection area, adjust the pH of the solution to 6-8 with 0.01mol / L NaOH and HCl respectively, and conduct the collection experiment of Escherichia coli in the air. Escherichia coli DH5α was cultivated to a concentration of 5×10 by conventional methods. 6 Finally, use 0.1mol / L phosphate solution to make 100,000 cells / ml solution, and then add to a volume of about 50m 3 In a confined space, the virus is sprayed in the air with an air distributor, and the specific number of microbial particles is calculated according to the reduction of the liquid in the sprayer (the concentration of microbial cells in the air is about 1000 / m 3 ). The volume of liquid in the liquid collection area is 15ml, the ratio of height to diameter is 3:1, and different methods are used to collect 0.1m 3 The Escherichia coli of the Escherichia coli was divided into two times to carry out the sampling detec...

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Abstract

The invention relates to an on-line collection and on-line automatic detection method of bacteria and viruses in air, by which in combination with a biochip or fluorescent quantitative PCR technology, possible pathogenic microorganisms in air can be quickly and sensitively detected. An on-line collection and on-line automatic detection device mainly comprises a negative-pressure air suction device, an air flow meter, an adsorbing solution and adsorbing particles. By use of the air suction device, through negative-pressure air suction, air runs through a liquid collection area at a constant air flow per minute; under the solution and the adsorbing particles in the solution, virus particles in air are collected in small-volume liquid; meanwhile, collected microorganisms are cracked to release DNAs (RNAs) of the microorganisms, and the microorganisms are adsorbed onto the adsorbing particles; then, through on-line filtration, the particles in the liquid are collected, the DNAs (RNAs) are eluted by using a commercially available DNA (RNA) eluent, then the microbial biomass is detected by a PCR technology, and the detected number of copies is divided by the volume of adsorbed air to obtain the detected number of microorganisms in unit air.

Description

technical field [0001] The invention belongs to the technical field of microorganism detection, in particular to an online collection and online automatic detection method for bacteria or viruses in the air. Background technique [0002] Airborne transmission is the main way for some pathogenic bacteria or viruses to spread, and it is also the most difficult way to detect and control in the current environment. [0003] From the SARS epidemic that had a huge impact on the world in 2003 to the H1N1 epidemic that affected China in 2009 and the bird flu outbreak in 2013, all are viral diseases that can be transmitted through the air. Take the SARS virus as an example: the virus mainly exists in the nasal mucus, sputum and saliva of the source of infection (human or animal), and is transmitted through air droplets and contact. When patients cough, sneeze, or even speak loudly, the virus will spread into the surrounding air with saliva droplets, dust particles, etc., and spread ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/26C12M1/34C12M1/36C12M1/12C12Q1/68
CPCC12Q1/24C12Q1/686
Inventor 王大平董承智
Owner SHANGHAI DEJU BIOTECH CO LTD
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