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Lactobacillus kefiranofaciens strain as well as screening culture medium, screening method and application thereof

A technology for screening medium and screening method, which is applied in the field of Lactobacillus kojimae strains and screening medium, can solve problems such as reducing diarrhea rate, and achieve the effects of simplified process, cost saving and strong screening ability

Inactive Publication Date: 2017-07-21
诺佰克(武汉)生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are also reports on the regulation of intestinal flora by a single microorganism. For example, the patent publication No. CN102994416 discloses the application of a strain of Bacillus licheniformis. The application of this strain is aimed at dogs and has not been disclosed to be effective in humans

Method used

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  • Lactobacillus kefiranofaciens strain as well as screening culture medium, screening method and application thereof
  • Lactobacillus kefiranofaciens strain as well as screening culture medium, screening method and application thereof
  • Lactobacillus kefiranofaciens strain as well as screening culture medium, screening method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] (1) Enrichment culture: Take a few Tibetan kefir grains and add them to the enrichment medium (E) according to the inoculum size of 10%, culture anaerobically at 35°C for 60 hours, and repeat passage for 3 times; the enrichment medium It is prepared from 14% goat milk powder aqueous solution or fresh goat milk and sterilized at 115°C for 20 minutes.

[0078](2) Primary screening and purification: Take the above-mentioned kefir emulsion containing kefir grains and grind it evenly in a sterile mortar, and inoculate the enriched liquid on the screening medium (S1) according to the inoculum size of 2%. Gradiently dilute on the plate, culture in an anaerobic box at 37°C for 72 hours, pick typical yellow colonies to another plate of primary screening medium (S1), and further pick yellow or milky white and viscous colonies to streak and purify , repeatedly to a single colony. The primary screening medium (S1) is: deproteinized goat milk whey solution 1000ml, glucose 10g, gala...

Embodiment 2

[0082] (1) Enrichment culture: Take a few Tibetan kefir grains and add them to the enrichment medium (E) according to the inoculum size of 8%, culture anaerobically at 30°C for 48 hours, and repeat passage twice; the enrichment medium It is prepared from 12% goat milk powder aqueous solution or fresh goat milk, which is sterilized at 115°C for 20 minutes.

[0083] (2) Primary screening and purification: Take 10-50 g of the above-mentioned kefir emulsion containing kefir grains, grind them evenly in a sterile mortar, and inoculate the enriched liquid on the screening medium ( Gradiently dilute on the plate of S1), culture in an anaerobic box at 30°C for 120 hours, pick typical yellow colonies to another plate of primary screening medium (S1), and further pick yellow or milky white and viscous colony strains Streak purification, repeated to a single colony. The primary screening medium (S1) is: deproteinized goat milk whey solution 1000ml, glucose 12g, galactose 10g, Tween-805m...

Embodiment 3

[0087] (1) Enrichment culture: Take a few Tibetan kefir grains and add them to the enrichment medium (E) according to the inoculum size of 5%, culture anaerobically at 30°C for 72 hours, and repeat passage twice; the enrichment medium It is prepared from 10% goat milk powder aqueous solution or fresh goat milk and sterilized at 115°C for 20 minutes.

[0088] (2) Primary screening and purification: Take 10-50 g of the above-mentioned kefir emulsion containing kefir grains, grind them evenly in a sterile mortar, and inoculate the enriched liquid on the screening medium ( Gradiently dilute on the plate of S1), culture in an anaerobic box at 35°C for 90 hours, pick typical yellow colonies to another plate of primary screening medium (S1), and further pick the strains with yellow or milky white and viscous colonies Streak purification, repeated to a single colony. The primary screening medium (S1) is: deproteinized goat milk whey solution 1000ml, glucose 11g, lactose 11g, Tween-80...

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Abstract

The invention relates to a lactobacillus kefiranofaciens strain as well as a screening culture medium, a screening method and application thereof, and belongs to the technical field of microorganisms. The lactobacillus kefiranofaciens subsp.kefiranofaciens has the collection number of CGMCCNo.2809. By the use of the screening culture medium of the lactobacillus kefiranofaciens strain for screening, lactobacillus kefiranofaciens can be obtained and is relatively high in repetitiveness; meanwhile, the screening time is shortened, and the screening process is simplified. When the strain is applied to preparing a medicine for preventing or treating constipation and diarrhea and enhancing the organ immunity, growth of healthful intestinal microflora can be effectively promoted, and multiplication of harmful bacteria is inhibited; moreover, when the strain is applied to improving the quality of cheese, the tensile strength of mozzarella cheese can be improved.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a strain of Lactobacillus kumis and its screening medium, screening method and application. Background technique [0002] Normal microorganisms in the human intestinal tract, such as bifidobacteria and lactobacilli, can synthesize a variety of vitamins necessary for human growth and development, and can also use protein residues to synthesize non-essential amino acids, and participate in the metabolism of carbohydrates and proteins. , magnesium, zinc and other mineral elements absorption. These nutrients play an important role in human health, and once they are deficient, they can cause various diseases. The intestinal flora is combined according to a certain ratio, and each bacteria restricts and depends on each other to form an ecological balance in terms of quality and quantity. Once the internal and external environment of the body changes, sensitive intes...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02A61K35/747A61P1/10A61P1/12A61P39/00A23L33/135A23C19/032C12R1/225
CPCA23C19/0325A61K35/747C12N1/02A23V2002/00A61K2035/115C12R2001/225C12N1/205A23V2400/11A23V2200/3204
Inventor 王艳萍邢竹青张阳
Owner 诺佰克(武汉)生物科技有限公司
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