Method for separating and purifying Scutellaria baicalensis polysaccharide extracted from radix scutellariae

A technology for separating and purifying Scutellaria baicalensis root is applied in the field of separating and preparing the effective polysaccharide of Scutellaria baicalensis, which can solve the problems of complicated operation, long production cycle, pollution of finished products, etc., and achieves the effects of safe and efficient process, low polysaccharide loss rate and simple operation.

Inactive Publication Date: 2017-07-25
XIAN BOTANICAL GARDEN SHAANXI PROV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods exist more or less: 1. The separation process uses solvents with clear toxicity and more impurities; 2. The process is not easy to scale up, not suitable for industrial production, the production cycle is long, the operation is complicated, and the production cost is high; 3. Toxic solvents not only put the finished product at risk of contamination, but also pose potential hazards to the production environment and operators

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Crush 200g of Scutellaria baicalensis into a coarse powder of 50-100 mesh, put it into a Soxhlet extractor, add 50mL of petroleum ether, and defat three times. Dry the degreased Scutellaria baicalensis powder and put it into a Soxhlet extractor, extract three times with 50mL deionized water at 100°C to obtain 200mL extract, cool naturally for later use; slowly add 2mL concentration to the 200mL extract Chitosan 2% acetic acid aqueous solution of 20mg / mL after leaving standstill 10min, in 10000rpm centrifugation discards precipitation, reclaims scutellaria baicalensis polysaccharide solution, vacuum distillation makes solution volume be 50mL; Add 267mL 95% ethanol to 50mL polysaccharide solution to make ethanol The final concentration is 80%. After standing for 10 minutes, filter and freeze-dry the precipitate to obtain the pure Scutellaria baicalensis polysaccharide. The recovery rate is 91.54%, the decolorization rate reaches 32.35%, and the protein removal rate reaches...

Embodiment 2

[0015] Crush 200g of Scutellaria baicalensis into a coarse powder of 50-100 mesh, put it into a Soxhlet extractor, add 50mL of petroleum ether, and defat three times. Dry the degreased Scutellaria baicalensis powder and put it into a Soxhlet extractor, extract three times with 50mL deionized water at 100°C to obtain 200mL extract, cool naturally for later use; slowly add 10mL concentration to the 200mL extract After being 20mg / mL chitosan 2% acetic acid aqueous solution, let it stand for 30min, discard the precipitate by centrifugation at 15000rpm, reclaim the Scutellaria baicalensis polysaccharide solution, and distill under reduced pressure to make the solution volume 40mL; add 213mL 95% ethanol to 40mL polysaccharide solution to make ethanol The final concentration is 80%. After standing for 10 minutes, filter and freeze-dry the precipitate to obtain pure Scutellaria baicalensis polysaccharide. The recovery rate is 80.43%, the decolorization rate reaches 82.35%, and the prot...

Embodiment 3

[0017] Crush 200g of Scutellaria baicalensis into a coarse powder of 50-100 mesh, put it into a Soxhlet extractor, add 50mL of petroleum ether, and defat three times. Dry the degreased Scutellaria baicalensis powder and put it into a Soxhlet extractor, extract three times with 50mL deionized water at 100°C to obtain 200mL extract, cool naturally for later use; slowly add 6mL concentration to the 200mL extract After being 20mg / mL chitosan 2% acetic acid aqueous solution, let it stand for 50min, discard the precipitate by centrifugation at 12000rpm, reclaim the Scutellaria baicalensis polysaccharide solution, and distill under reduced pressure to make the solution volume 100mL; add 533mL 95% ethanol to 100mL polysaccharide solution to make ethanol The final concentration is 80%. After standing for 10 minutes, filter and freeze-dry the precipitate to obtain the pure Scutellaria baicalensis polysaccharide. The recovery rate is 80.65%, the decolorization rate reaches 73.53%, and the...

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Abstract

The invention discloses a method for separating and purifying Scutellaria baicalensis polysaccharide extracted from radix scutellariae. The method utilizes a water system method. The method comprises extracting functional crude polysaccharide from radix scutellariae, flocculating the crude polysaccharide solution through a chitosan-acetic acid aqueous solution, carrying out centrifugal separation, carrying out concentration, carrying out ethanol precipitation and carrying out freeze-drying so that purification of crude Scutellaria baicalensis polysaccharide is realized. The method is simple and easy, has good operability, has simple production processes and realizes earlier fast impurity removal of crude polysaccharide of Scutellaria baicalensis.

Description

technical field [0001] The invention relates to a production process for separating and preparing effective Scutellaria baicalensis polysaccharides from traditional Chinese medicine powder Scutellaria baicalensis. Background technique [0002] Scutellaria baikalensis Georgi is one of the important components of traditional Chinese medicine in my country. As a traditional Chinese medicinal material, its root is mainly used for anti-viral infection, bacterial infection, anti-tumor, scavenging free radicals, regulating immunity, etc. It also has the effect of lowering cholesterol and blood pressure. effect. A variety of active ingredients contained in it have gradually been discovered by people, including baicalin, wogonin, baicalein, wogonin, baicalin polysaccharides, etc. Among them, Scutellaria baicalensis polysaccharide has not received enough attention at home and abroad due to its complicated separation and purification methods. As a member of the bioactive polysaccharide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003C08B37/00
Inventor 崔新爱周军辉刘国宇毛祝新石新卫路强强
Owner XIAN BOTANICAL GARDEN SHAANXI PROV
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