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Live bacterium detection method based on photoacoustic imaging technology

A technology of photoacoustic imaging and detection methods, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, measurement devices, etc., can solve the problems of high cost, time-consuming, insufficient clinical practicability, etc. Simple operation, low cost effect

Active Publication Date: 2017-07-28
XIAMEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these detection techniques have the advantages of high accuracy, they are time-consuming, costly, and unable to detect the real-time status of live bacteria, and their clinical practicability is relatively insufficient

Method used

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  • Live bacterium detection method based on photoacoustic imaging technology
  • Live bacterium detection method based on photoacoustic imaging technology
  • Live bacterium detection method based on photoacoustic imaging technology

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Experimental program
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Embodiment 1

[0030] see Figure 1~4 , a single live bacteria detection method based on photoacoustic imaging technology, the steps are as follows:

[0031] (1) Staining culture of a single live bacteria

[0032] The photoacoustic imaging enhancer is added to the culture solution containing live bacteria for incubation, and the bacterial culture solution is selected according to the growth conditions of different bacterial strains. In order to ensure the viability of the bacteria and promote the phagocytosis of the probe by the bacteria, the temperature is 37°C, the rotation speed is 200rmp, and the time is 8-12h. Photoacoustic enhancer one is: indocyanine green, the concentration of which is 0.4 mg / mL. It is characterized in that the absorption peak is at a wavelength of 790nm, which is within the optimum wavelength range of 680-980nm for photoacoustic imaging instruments, and has no killing effect on bacteria. The second sound enhancer is: Prussian blue, its concentration is 0.4mg / mL. ...

Embodiment 2

[0035] see Figure 5-7 , a method for the mixed detection of two live bacteria based on photoacoustic imaging technology, the steps are as follows:

[0036] (1) Separate staining and culture of single live bacteria

[0037] Two different photoacoustic imaging enhancers were added to the culture solution containing probiotics or pathogenic bacteria for incubation, and the optimal culture solution was selected according to the growth conditions of different strains. In order to ensure the viability of the bacteria and promote the phagocytosis of the probe by the bacteria, the temperature is 37°C, the rotation speed is 200rmp, and the time is 8-12h. Photoacoustic enhancer one is: indocyanine green, the concentration of which is 0.4 mg / mL. It is characterized in that the absorption peak is at a wavelength of 790nm, which is within the optimum wavelength range of 680-980nm for photoacoustic imaging instruments, and has no killing effect on bacteria. The second sound enhancer is:...

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Abstract

The invention discloses a live bacterium detection method based on a photoacoustic imaging technology, and relates to live bacterium detection. The live bacterium detection method based on the photoacoustic imaging technology can be used for detecting a single type of bacteria or a mixture of two types of bacteria, rapidly and conveniently detecting the relative concentrations of probiotics and pathogens, and providing an important reference value for clinical guidance and therapy. The live bacterium detection method I comprises the following steps: in order to promote the bacteria to phagocytize a probe, adding a photoacoustic imaging enhancer into a live bacterium culture solution, incubating to obtain a bacterium solution, taking 150 microliters of the bacterium solution, adding the bacterium solution into a new EP tube with the volume of 200 microliters, and carrying out the live bacterium detection method based on the photoacoustic imaging technology. The live bacterium detection method II comprises the following steps: in order to ensure the bacterial activity and promote the bacteria to phagocytize the probe, adding two different photoacoustic imaging enhancers into a probiotic culture solution and a pathogen culture solution respectively, incubating, carrying out the live bacterium detection method based on the photoacoustic imaging technology, and according to an obtained photoacoustic intensity value, calculating the relative concentration ratio of the probiotics to the pathogens.

Description

technical field [0001] The invention relates to the detection of living bacteria, in particular to a method for detecting living bacteria based on photoacoustic imaging technology. Background technique [0002] The microbiome is an important component of the human intestinal system. The vast majority of microorganisms in the human gut are hundreds of trillions of living bacteria. These large numbers of bacteria can be roughly divided into three categories: probiotics, pathogenic bacteria and neutral bacteria. The homeostasis of the flora, especially the balance of probiotics and pathogenic bacteria, maintains the health of the human body. Probiotics mainly include: Lactobacillus, Clostridium butyricum, Bifidobacterium, Enterococcus faecalis, Enterococcus faecium, actinomycetes, yeast, etc., while pathogenic bacteria in the human body mainly include: pathogenic Escherichia coli, Salmonella , Shigella, Proteus, Bacteroides fragilis, Yersinia, etc. [0003] Host-microbe sym...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/17C12Q1/04C12Q1/10
CPCC12Q1/04C12Q1/10G01N21/1702Y02A50/30
Inventor 施颖黄肖肖聂立铭任建林
Owner XIAMEN UNIV