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Method for Determination of Fingerprint of Huagaisan Granules

A technology of fingerprints and determination methods, which is applied in the field of detection of traditional Chinese medicine preparations, can solve the problems of not being able to systematically and comprehensively reflect the internal quality of Huagaisan granules, and achieve the effects of enhancing the overall comparison, making it simple, and realizing the chromatographic conditions

Active Publication Date: 2020-05-05
太极集团重庆桐君阁药厂有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there is no literature report on the research on the fingerprint of Huagaisan particles in China.
Dong Ziliang and others reported the fingerprint detection method of Huagaisan traditional decoction in "Journal of Chinese Herbal Medicine" (2016, 47(3):425-429), but after a lot of experimental research, this method is not suitable for the quality of Huagaisan granule products Control, cannot systematically and comprehensively reflect the intrinsic quality of Huagaisan particles

Method used

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  • Method for Determination of Fingerprint of Huagaisan Granules
  • Method for Determination of Fingerprint of Huagaisan Granules
  • Method for Determination of Fingerprint of Huagaisan Granules

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0038] Experimental example 1, optimization of chromatographic conditions

[0039] 1. Inspection of scanning methods

[0040] The fingerprints of Huagaisan particles under different wavelength conditions were compared, and two scanning methods were selected: 190-400nm full-wavelength scanning (extraction wavelength 278nm) and 278nm single-wavelength scanning for comparison. The obtained chromatogram results are shown in figure 1 shown.

[0041] From the perspective of the separation of spectral peaks, the peak shape of the 278nm single-wavelength scanning image is better, the separation of each peak is good, and the baseline is stable, which is better than the wavelength 278nm image extracted after the full-wavelength scanning of 190-400nm, so the 278nm single-wavelength is selected as the canopy. Scanning wavelengths for the fingerprints of scattered particles.

[0042] 2. Selection of chromatographic column

[0043] Under the same chromatographic conditions, select: Therm...

experiment example 2

[0059] Experimental example 2, Huagai San fingerprint method verification

[0060] 1. Fingerprint detection

[0061] According to the optimized detection conditions in the above experiment, the fingerprint detection of Huagaisan particles was carried out, including:

[0062] Take an appropriate amount of Huagai powder granule sample, grind it finely, weigh 0.50g, add 25ml of 50% methanol, ultrasonicate for 30min, let cool, make up for weight loss, shake well, and filter to obtain the test solution.

[0063] According to high performance liquid chromatography, octadecylsilane bonded silica gel is used as filler, acetonitrile is used as mobile phase A, and 0.01% formic acid water is used as mobile phase B; the flow rate is controlled at 0.8-1.2ml / min; column temperature is 27°C ; Detection wavelength: 278nm; Carry out gradient elution according to the following procedure: from 0 to 25 min, the volume ratio of mobile phase A: mobile phase B is from 2%: 98% → 7%: 93%; from 25 to ...

experiment example 3

[0089] Experimental Example 3: Identification of Chromatographic Peaks in HPLC Fingerprint of Huagaisan Granules

[0090] By investigating the retention time and ultraviolet spectrum of different reference substances, it was identified that No. 8 peak (75.59min) was liquiritin, No. 11 peak (84.434min) was hesperidin, No. 12 peak (85.568min) in the HPLC fingerprint of Huagaisan Granules. ) is rosmarinic acid. According to the optical information given by the spectrogram, it is found that the ultraviolet spectrum of the Huagaisan particle chromatogram is consistent with the corresponding known reference substance chromatographic peak. The obtained chromatogram results are shown in Image 6 As shown, the source analysis results of the characteristic peaks of Huagai San fingerprint spectrum are shown in Table 7.

[0091] Table 7. The source analysis results of the characteristic peaks of Huagai San fingerprint spectrum

[0092]

[0093]

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Abstract

The invention relates to a method for detecting the fingerprint of Huagaisan granules. The method comprises the following steps: taking the Huagaisan granules, grinding the granules, adding a methanol solution, carrying out ultrasonic dissolving, cooling the obtained solution, adding the methanol solution to supplement the lost weight, shaking up the solution, filtering the solution, and collecting the obtained filtrate to obtain a Huagaisan granule sample; and carrying out elution under the following conditions: octadecylsilane chemically bonded silica is used as the chromatographic column of a packing, the column temperature is 27 DEG C, the flow velocity is 0.8-1.2 ml / min, the detection wavelength is 278 nm, and the mobile phase is acetonitrile and formic acid water with the formic acid mass fraction of 0.01%. The method is used to establish the fingerprint of Huagaisan granules, and provides a new method for the quality control and the authenticity identification of the Huagaisan granules, and the fingerprint can completely reflect the substance information of a traditional Chinese medicine, and enhances the visual comparison of fingerprint peaks.

Description

technical field [0001] The invention belongs to the field of detection of traditional Chinese medicine preparations, and relates to a method for determining the fingerprint of Huagaisan particles. Background technique [0002] The composition of traditional Chinese medicine is complex, and the active part is often not a single component. Using a single component as a quality control indicator has become less and less suitable for the quality control requirements of traditional Chinese medicine. Therefore, traditional Chinese medicine fingerprint technology came into being. [0003] Traditional Chinese medicine fingerprint technology originates from fingerprint identification, and uses modern information technology and quality analysis methods to comprehensively reflect the types and quantities of chemical components contained in traditional Chinese medicine. For Chinese medicinal materials, fingerprints can be used to identify the authenticity and judge the quality; for Chi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 秦少容黄静彭涛董自亮卿玉玲官柳冉亚东原欢欢禹奇男刘世琪彭世陆
Owner 太极集团重庆桐君阁药厂有限公司