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Method for improving stability of glutamine in stem cell culture medium

A technology for glutamine and stabilizing performance, which is applied in the field of improving glutamine stability in stem cell culture medium, can solve problems such as unfavorable cell growth, cytotoxicity, and difficulty in dissolving, and achieves an expanded application range, enhanced dissolving speed, Effects that are not easily decomposed

Inactive Publication Date: 2017-08-08
句容亿格纳米材料厂
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But on the other hand, because glutamine is very unstable in the solution, it is easy to decompose into amine substances, which has a toxic effect on cells and is not conducive to cell growth.
Moreover, glutamine is not easy to dissolve at room temperature, so the usual treatment method when preparing a medium containing glutamine is to dissolve it in a constant temperature working water bath, but this method will further promote the insoluble glutamine. Stability is stimulated

Method used

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  • Method for improving stability of glutamine in stem cell culture medium

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Effect test

Embodiment 1

[0017] A kind of stem cell medium, its formulation raw material contains glutamine, in order to improve the chemical stability of glutamine, it is optimized, before the medium containing glutamine is prepared, glutamine and nanomaterials Composite nanogel particles with a particle size of 0.1 μm are first prepared and then added to the medium for preparation;

[0018] The preparation method of the composite nanogel particles is as follows: Glutamine and water-soluble chitosan derivatives are mixed with a mass ratio of 3:1 and placed in a supercritical reactor device, the reaction temperature is set to 40 ° C, and the reaction is carried out for 15 minutes. , add nano-zinc oxide powder, raise the temperature to 800°C, continue the treatment for 1h, take it out after cooling to room temperature and place it in a low-temperature plasma reaction device, add an anhydrous ethanol solution with 5 times the amount of solid mixture, and the reaction temperature is -6°C, After reacting ...

Embodiment 2

[0020] A kind of stem cell medium, its formulation raw material contains glutamine, in order to improve the chemical stability of glutamine, it is optimized, before the medium containing glutamine is prepared, glutamine and nanomaterials Composite nanogel particles with a particle size of 0.1 μm are first prepared and then added to the medium for preparation;

[0021] The preparation method of the composite nanogel particles is as follows: Glutamine and water-soluble chitosan derivatives are mixed with a mass ratio of 3:1 and placed in a supercritical reactor device, the reaction temperature is set to 70 ° C, and the reaction is carried out for 25 minutes. , add nano-zinc oxide powder, raise the temperature to 850°C, continue the treatment for 2h, take it out after cooling to room temperature and place it in a low-temperature plasma reaction device, add an anhydrous ethanol solution that is 8 times the amount of solid mixture, and the reaction temperature is -1°C, After reacti...

Embodiment 3

[0023] A kind of stem cell medium, its formulation raw material contains glutamine, in order to improve the chemical stability of glutamine, it is optimized, before the medium containing glutamine is prepared, glutamine and nanomaterials Composite nanogel particles with a particle size of 0.1 μm are first prepared and then added to the medium for preparation;

[0024] The preparation method of the composite nanogel particles is as follows: Glutamine and water-soluble chitosan derivatives are mixed with a mass ratio of 3:1 and placed in a supercritical reactor device, the reaction temperature is set to 55 ° C, and the reaction is carried out for 20 minutes. , add nano-zinc oxide powder, heat up to 830°C, continue to process for 1.5h, take it out after cooling to room temperature and place it in a low-temperature plasma reaction device, add an anhydrous ethanol solution that is 7 times the amount of solid mixture, and the reaction temperature is -3°C After reacting for 25 minute...

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Abstract

The invention discloses a method for improving stability of glutamine in a stem cell culture medium. The method has the advantages that before the culture medium containing the glutamine is prepared, the glutamine and a nanometer material are prepared into composite nanometer gel particles, and then the composite nanometer gel particles are added into the culture medium to prepare; the chemical stability of the glutamine is effectively improved, and the easiness in decomposing is avoided; the hydrophilic solubility with distilled water is improved, the additional heating for dissolving is not needed in the preparation process of the culture medium, the dissolving speed is effectively improved, the problem of instability of chemical property of the glutamine is solved, the culture and accuracy of subsequent test result are effectively guaranteed, the error is reduced, and the culture survival rate of the stem cell culture medium and the accuracy of application study are improved; in addition, the external heating operation is avoided, the destroy to chemical property of other nutrient components is avoided, and the application range is widened.

Description

technical field [0001] The invention relates to the field of microorganism culture, in particular to a method for improving the stability of glutamine in stem cell culture medium. Background technique [0002] Culture medium is the nutrient substrate necessary for the growth, reproduction or accumulation of metabolites of microorganisms or animal or plant cells in the process of microbial fermentation or animal and plant cell culture. The components of a general medium include: carbon sources, nitrogen sources, minerals, and other essential substances, such as substances that cannot be synthesized by certain organisms themselves, such as certain amino acids, vitamins or nucleotides. In addition, pH buffers are often added to the medium to maintain a stable pH. [0003] Glutamine is an essential amino acid for cells to synthesize nucleic acids and proteins. When there is a lack of glutamine in the culture medium, the cells will grow poorly and die. Therefore, an appropriate...

Claims

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Application Information

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IPC IPC(8): C12N5/00
CPCC12N5/0018C12N2500/22C12N2500/32C12N2501/90
Inventor 潘峥嵘
Owner 句容亿格纳米材料厂
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