Method for extracting SOD (Superoxide Dismutase) refined enzyme from SOD enriched plants (triticale and rice oil bran powder) after biological fermentation

A technology of biological fermentation and rice oil bran, applied in the biological field, can solve the problems of uncontrollable dosage, unquantifiable, low sod content, etc.

Inactive Publication Date: 2017-08-08
夏胜芳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, SOD enzymes widely exist in the animal and plant kingdoms in the prior art, and they are not resistant to high temperatures (above 40° C.), and are easil

Method used

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  • Method for extracting SOD (Superoxide Dismutase) refined enzyme from SOD enriched plants (triticale and rice oil bran powder) after biological fermentation
  • Method for extracting SOD (Superoxide Dismutase) refined enzyme from SOD enriched plants (triticale and rice oil bran powder) after biological fermentation
  • Method for extracting SOD (Superoxide Dismutase) refined enzyme from SOD enriched plants (triticale and rice oil bran powder) after biological fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] 1. Choose oily pine needles (Pinus tabuliformis Carrire) 30 kg and cut into inch sections

[0061]

[0062] Mix evenly according to the above ratio, a total of 100 kilograms.

[0063] 2. Add 300 kg of water and use a stone mill for wet grinding;

[0064] 3. The collected oar liquid first passes through a 100-mesh filter, a 500-mesh filter, and an 800-mesh filter to collect the filtrate.

[0065] 4. Use molecular sieves (American GE (Sweden amersham) 17-0612-10SEPHACRYL S-100HR, 150ML (gel filtration filler Sephacryl), condition pH7.8, 50mmol / LPBS for elution and control the flow rate to 42mL / h) for sod molecules After screening, sod crude enzyme is obtained.

[0066] 5. Heating at 10°C to 40°C, standing for 72 hours, and passing through the above-mentioned sod molecular sieves and conditions to obtain a new sod filtrate.

[0067] 6. Low-temperature (-18 to -198° C.) spray freeze-drying, and the collected dry powder is the new product sod essence, named Laobing Yiw...

Embodiment 2

[0071] Embodiment 2 (dry method)

[0072] 1. Choose oily pine needles (Pinus tabuliformis Carrire) 30 kg and cut into inch sections

[0073]

[0074] Mix evenly according to the above ratio, a total of 100 kilograms.

[0075] 2. Dry at 40-58 degrees, and then pulverize (1500 mesh) with low-temperature airflow ultrafine pulverization;

[0076] 3. The collected raw material powder. After adding 65% of water, ferment at 10-40 degrees for 3 days, add 300 kg of water to obtain liquid filtration

[0077] 4. Use molecular sieves for sod molecular sieves (American GE (Amersham, Sweden) 17-0612-10SEPHACRYL S-100HR, 150ML (gel filtration filler Sephacryl), conditions pH 7.8, 50mmol / LPBS for elution and control flow rate 42mL / h) After screening, sod crude enzyme is obtained.

[0078] 5. Heating at 10° C. to 40° C., standing for 72 hours, and passing through the sod molecular sieve and conditions described in Example 1 to obtain new sod filtrate.

[0079] 6. Low-temperature (-18 t...

Embodiment 3

[0080] Embodiment 3 (alcohol extraction method)

[0081] 1. Choose oily pine needles (Pinus tabuliformis Carrire) 30 kg and cut into inch sections

[0082]

[0083] Mix evenly according to the above ratio, totaling 100 kilograms.

[0084] 2. Dry at 40-58 degrees, and then pulverize (500 mesh) with low-temperature airflow ultrafine pulverization;

[0085] 3. The collected raw material powder. Add 300 kilograms of 52-degree edible liquor and soak for 3 days at 10-40 degrees, stir and filter to obtain a supernatant, which is separated with a high-speed centrifuge.

[0086] 4. Use molecular sieves for sod molecular sieves (American GE (Amersham, Sweden) 17-0612-10SEPHACRYL S-100HR, 150ML (gel filtration filler Sephacryl), conditions pH 7.8, 50mmol / LPBS for elution and control flow rate 42mL / h) After screening, sod crude enzyme is obtained.

[0087] 5. Heating at 10°C-40°C, standing for 72 hours, and passing through the above-mentioned sod molecular sieve and conditions to o...

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PUM

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Abstract

The invention provides a method for extracting an SOD (Superoxide Dismutase) refined enzyme from SOD enriched plants (triticale, rice oil bran powder, black garlic, hippophae rhamnoides, rosa roxburghii, pinus tabuliformis carrire and pinus tabuliformis carrire) after biological fermentation. The method comprises the following steps of selecting SOD enriched raw materials, mixing the SOD enriched raw materials according to a proportion, and fermenting an obtained mixture for 3 days at 10 to 40 DEG C; carrying out wet grinding, collecting pulp, centrifuging the pulp at a high speed, breaking cell walls, and filtering by a molecular sieve, so as to subsequently obtain an SOD crude enzyme; heating the SOD crude enzyme, then putting the heated SOD crude enzyme for a period of time, and then filtering the SOD crude enzyme by using the molecular sieve, and treating filtrate by using a low-temperature spray freeze drying method; collecting dry powder, so as to obtain the SOD refined enzyme. The SOD refined enzyme extracted by the method is an active enzyme which can be resistant to a high temperature; the enzyme activity is not destroyed; the SOD enzyme activity is maximized; the maximization of the absorption and the utilization of a body on the SOD refined enzyme is realized.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for extracting SOD essence enzyme from SOD-rich plants (plants such as triticale and rice oil bran powder) after biological fermentation. Background technique [0002] SOD is the abbreviation of Super Oxide Dismutase. The Chinese name is superoxide dismutase. It is an important antioxidant enzyme in organisms and is widely distributed in various organisms, such as animals, plants, and microorganisms. SOD has special physiological activity and is the primary substance for scavenging free radicals in organisms. The level of SOD in the living body means an intuitive indicator of aging and death; it has been confirmed that there are as many as 60 kinds of diseases caused by oxygen free radicals. It can resist and block the damage to cells caused by oxygen free radicals, repair damaged cells in time, and restore the damage to cells caused by free radicals. Due to th...

Claims

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Application Information

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IPC IPC(8): C12N9/02
CPCC12N9/0089C12Y115/01001
Inventor 夏胜芳杨瑞王健霞
Owner 夏胜芳
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