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High-polymer adsorbing material for separating and purifying vitamin B12 from fermentation liquid

A technology for separation, purification and adsorption materials, which is applied in the direction of sugar derivatives, sugar derivatives, organic chemistry, etc., to avoid the problem of toxic monomer residues, improve the safety of use, and achieve the effect of efficient desorption

Inactive Publication Date: 2017-08-11
MUDANJIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide a method for separating and purifying vitamin B from fermentation broth with large adsorption capacity, fast adsorption speed and easy desorption. 12 polymer adsorption material

Method used

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  • High-polymer adsorbing material for separating and purifying vitamin B12 from fermentation liquid

Examples

Experimental program
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Effect test

Embodiment 1

[0028] (1) Preparation of macroporous adsorption resin: add 45 parts of hydrogenated abietyl alcohol methacrylate, 30 parts of pentaerythritol triacrylate, 15 parts of dimethylaminoethyl acrylate, 40 parts of nanoporogen and 0.5 parts of initiator, mixed thoroughly, ultrasonically treated at ultrasonic frequency 40kHz, output power 200W for 15min, then microwaved at microwave frequency 2450MHz, output power 700W for 10min, and left at 0-5°C for 30min, after mixing evenly Continue microwave treatment for 10 minutes, then let stand at -5-0°C for 15 minutes, mix well and then microwave again for 10 minutes, cool down the mixture at a cooling rate of 5°C / min to 40-45°C for 1 hour, then mix Liquid-solid-liquid separation, the obtained solid phase was washed three times with warm water at 40-45°C to obtain white balls, and finally the white balls were vacuum-dried at 60-65°C to obtain macroporous adsorption resin;

[0029] (2) Pretreatment of macroporous adsorbent resin: add 2 parts...

Embodiment 2

[0034] (1) Preparation of macroporous adsorbent resin: add 50 parts of hydrogenated abietyl alcohol methacrylate, 40 parts of pentaerythritol triacrylate, 10 parts of dimethylaminoethyl acrylate, 50 parts of nanoporogen and 0.5 parts of initiator, mixed thoroughly, ultrasonically treated at ultrasonic frequency 40kHz, output power 200W for 15min, then microwaved at microwave frequency 2450MHz, output power 700W for 10min, and left at 0-5°C for 30min, after mixing evenly Continue microwave treatment for 10 minutes, then let stand at -5-0°C for 15 minutes, mix well and then microwave again for 10 minutes, cool down the mixture at a cooling rate of 5°C / min to 40-45°C for 1 hour, then mix Liquid-solid-liquid separation, the obtained solid phase was washed three times with warm water at 40-45°C to obtain white balls, and finally the white balls were vacuum-dried at 60-65°C to obtain macroporous adsorption resin;

[0035] (2) Pretreatment of macroporous adsorbent resin: add 2 parts ...

Embodiment 3

[0040] (1) Preparation of macroporous adsorbent resin: add 50 parts of hydrogenated abietyl alcohol methacrylate, 40 parts of pentaerythritol triacrylate, 10 parts of dimethylaminoethyl acrylate, 50 parts of nanoporogen and 0.5 parts of initiator, mixed thoroughly, ultrasonically treated at ultrasonic frequency 40kHz, output power 200W for 15min, then microwaved at microwave frequency 2450MHz, output power 700W for 10min, and left at 0-5°C for 30min, after mixing evenly Continue microwave treatment for 10 minutes, then let stand at -5-0°C for 15 minutes, mix well and then microwave again for 10 minutes, cool down the mixture at a cooling rate of 5°C / min to 40-45°C for 1 hour, then mix Liquid-solid-liquid separation, the obtained solid phase was washed three times with warm water at 40-45°C to obtain white balls, and finally the white balls were vacuum-dried at 60-65°C to obtain macroporous adsorption resin;

[0041] (2) Pretreatment of macroporous adsorbent resin: add 2 parts ...

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Abstract

The invention discloses a high-polymer adsorbing material for separating and purifying a vitamin B12 from fermentation liquid, and relates to the technical field of biological medicine extraction. A preparing method of the high-polymer adsorbing material includes the following steps of (1) macroporous adsorption resin preparing; (2) macroporous adsorption resin pretreating; (3) macroporous adsorption resin modifying. The vitamin-B12 adsorbing capacity of the macroporous adsorption resin prepared with the method is 18.5 mg / g or above, efficient desorbing of the vitamin B12 can be achieved after washing is carried out through a 65% methanol solution, the desorption rate is 98.5% or above, the purity of the prepared vitamin B12 is 58% or above, and the macroporous adsorption resin is suitable for separating and purifying the vitamin B12 from the fermentation liquid accordingly.

Description

[0001] Technical field: [0002] The invention relates to the technical field of biomedicine purification, in particular to a method for separating and purifying vitamin B from fermentation broth 12 polymeric adsorption material. [0003] Background technique: [0004] Vitamin B 12 , also known as cobalamin, is a general term for a class of compounds containing corrin rings. Currently, vitamin B 12 The preparation method is mainly divided into two kinds of chemical synthesis method and fermentation production method. Among them, chemical synthesis produces vitamin B 12 The cost is high, the by-products are many, and it is difficult to purify. Vitamin B 12 The fermentation production methods mainly include antibiotic waste liquid extraction, actinomycetes fermentation, propionic acid bacteria fermentation and transgenic E.Coli fermentation production. Pseudomonas denitrification is the new B vitamin emerging this year 12 The strain produced by aerobic fermentation has th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/26B01J20/30C08F220/18C08F222/16C08F220/34C08F2/46C08F8/46C08F8/30C08J9/26C07H23/00C07H1/06
CPCB01J20/06B01J20/262C07H1/06C07H23/00C08F2/46C08F8/30C08F8/46C08F220/18C08J9/26C08J2201/0422C08J2201/0424C08J2333/08C08F222/16C08F220/34
Inventor 梁军胡秉娇闫磊孙凯晁岳刚徐建忠桂金秋蔡克瑞王妍宋铁军
Owner MUDANJIANG MEDICAL UNIV
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