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Method for detecting thiosulfate ions in blood or urine

A thiosulfate and urine technology, applied in the field of chemical detection, can solve problems such as low detection limit, and achieve the effects of good precision and accuracy, good linear correlation and good specificity

Inactive Publication Date: 2017-08-11
司法部司法鉴定科学技术研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the determination of thiosulfate ions mostly uses high performance liquid chromatography (Yao Shouren, Yu Shukun, Luo Siquan. High-speed liquid chromatography analysis of sulfite, sulfide and thiosulfate ions in water[J].Environmental Science ,1981(6)), ion chromatography (Ren Huazhong, Feng Jiafu, Wu Chaojun. Determination of sulfite and sulfate in sodium thiosulfate for injection by ion chromatography[J]. Huaxi Pharmaceutical Journal, 2015,30(1) ), electrocatalytic oxidation and ion chromatography detection (Ji-Ming X U, Wang Y P, Xian Y Z, et al.Electrocatalytic Oxidation and IonChromatography Detection of S 2 o 3 2- , SO 3 2- , I-and SCN-at Glassy Carbon Electrodewith Functionalized Multi-Wall Carbon Nanotubes Film[J].Chemical Research in Chinese Universities, 2004,20(5):529-533), spectrophotometry (Xiong Jianhua, UV spectrophotometry Sodium thiosulfate content in the solution [J]. Medical Herald, 2004, 23 (11): 866-866), capillary electrophoresis (Jeffrey M I, Brunt S D.The quantification of thiosulfate and polythionates in gold leachsolutions and on anion exchange resins [J].Hydrometallurgy,2007,89(1–2):52-60) and other methods, however, these methods are only applicable to aqueous solutions such as sewage and waste water, and are not suitable for thiolation in complex biological matrices such as blood and urine. Determination of sulfate ions; and some methods therein also have the minimum detection limit (LOD), the minimum quantification limit (LOQ) is not low enough, the precision and accuracy are not good enough defects

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  • Method for detecting thiosulfate ions in blood or urine
  • Method for detecting thiosulfate ions in blood or urine
  • Method for detecting thiosulfate ions in blood or urine

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Embodiment 1

[0068] On a certain day in December 2015, two workers Wu XX and Ji XX were found dead in the sewage well of the bamboo pulp processing factory. After monitoring by the environmental department, the concentration of hydrogen sulfide in the air in the well was 6ppm. As determined by the method of the present invention, the blood thiosulfate ion concentrations of Wu XX and Ji XX were 0.17 μmol / mL and 0.19 μmol / mL, respectively.

Embodiment 2

[0070] On a certain day in April 2016, in the production workshop of a biotechnology company in Daming County, Hebei Province, 3 people died due to hydrogen sulfide leakage. According to the description of the surviving workers, Chen XX and Hou XX found Dong X lying on the ground, stepped forward to check, shouted and pulled Dong X, and then they also fell to the ground. As determined by the method of the present invention, the concentrations of thiosulfate ions in the blood of Chen XX, Hou XX and Dong X were 0.24 μmol / mL, 0.24 μmol / mL and 0.31 μmol / mL, respectively.

Embodiment 3

[0072] On a certain day in July 2016, a poisoning accident occurred in a sewage pipe inspection well in Wenzhou City. Li X, a worker who was working, fell into a coma during the operation and died when he arrived at the hospital. As determined by the method of the present invention, the concentration of thiosulfate ion in Li X's blood was 0.23 μmol / mL.

[0073] According to the current hydrogen sulfide poisoning death case report, the thiosulfate ion in the blood is not detected to within the range of 0.648 μmol / mL, and in the urine is not detected to within the range of 2.669 μmol / mL; In the actual cases detected by the detection method, the concentration of thiosulfate ion in the blood of the above-mentioned 5 poisoners was 0.17-0.31 μmol / mL, which proves that the detection method of the present invention is sufficient to meet the determination requirements of identifying actual biological samples.

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Abstract

The invention provides a method for detecting thiosulfate ions in blood or urine. The method comprises the steps as follows: (1) pretreatment of a sample: thiosulfate ions are subjected to derivatization to form pentafluorobenzyl disulfide, to be specific, a blood sample or a urine sample is placed in a glass test tube in which an acetone solution of pentafluorobenzyl bromide, an L-ascorbic acid aqueous solution and a NaCl aqueous solution are added and then subjected to vortex in a vortex oscillator, an ethyl acetate solution of iodine and an ethyl acetate solution of 1,3,5-tribromobenzene serving as an internal standard are added to the glass test tube, the mixture is subjected to vortex continuously, left to stand and centrifuged, and a supernatant is taken and injected; (2) GC-MS analysis. Compared with the prior art, the method has better specificity, linear correlation, precision and accuracy, meanwhile, LOD and LOQ are low enough, thus, the method is very applicable to determination of the thiosulfate ions in blood or urine so as to indirectly determine the poisoning condition of hydrogen sulfide, and the method can be widely applied and has considerable market potential.

Description

technical field [0001] The invention belongs to the field of chemical detection, and relates to a method for detecting ions in blood or urine derived from a human body, in particular to a method for detecting thiosulfate ions in blood or urine. Background technique [0002] Hydrogen sulfide (H 2 S) is a colorless highly toxic gas with a pungent smell of rotten eggs. After the human body inhales highly toxic hydrogen sulfide gas, it will damage the alveolar capillaries and destroy the alveolar surfactant, which can cause acute damage to the respiratory mucosa and acute pulmonary edema; inhalation of a small amount of high-concentration hydrogen sulfide can cause respiratory arrest and cause death like electric shock. Since hydrogen sulfide exists in many industrial production and living environments, such as mining, papermaking, wastewater treatment, sewer cleaning, etc., hydrogen sulfide poisoning accidents occur frequently. [0003] The determination of hydrogen sulfide i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 向平沈敏施妍强火生
Owner 司法部司法鉴定科学技术研究所