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Method for Bacillus subtilis and Bacillus coagulans mixed fermentation, and use method thereof

A technology of Bacillus coagulans and Bacillus subtilis, which is applied in the field of mixed fermentation of Bacillus subtilis and Bacillus coagulans, can solve the problems of inconspicuousness and high price, shorten the production process cycle, enhance immunity and disease resistance, The effect of improving adaptability

Inactive Publication Date: 2017-08-15
西安鑫汉宝生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Bacillus subtilis ( Bacillus subtilis ), which is a mesophilic aerobic spore-forming rod-shaped bacterium (G 十 ), the spores are oval or columnar, mesogenic or partial mesogenic, even if the cysts are enlarged, it is not obvious; it is mostly used in aquatic products, livestock and poultry breeding, and the addition method is mostly used to make bacterial powder, but the price is more expensive

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: in the embodiment of the present invention, the culture of preparation comprises the following steps:

[0031] (1) Use the basic peptone beef extract liquid medium to rejuvenate the production bacteria: pick a ring of slanted strains and inoculate them in the seed liquid medium, the medium capacity of 150ml Erlenmeyer flask is 50ml, 37~45℃, 200~220r / min Shake flask culture for 20~24h, this is the primary seed liquid, this seed liquid is inoculated into the secondary seed liquid culture medium with 2% inoculation amount, 1000ml Erlenmeyer bottle with 200ml liquid volume, 37~45℃, 200~ Shake flask culture at 220r / min for 20~24h; Among them, the composition of the basic liquid medium is: peptone: 10~15 g / L, beef extract: 5~10 g / L, sodium chloride: 5 g / L, adjust the pH to 7.0~7.2; Secondary seed Bacillus subtilis culture solution composition: glucose: 5~15 g / L, peptone: 10~15 g / L, yeast extract: 10~20 g / L, K 2 HPO 4 : 0.5~1g / L, Na 2 HPO 4 : 3~5g / L, adjuste...

Embodiment 2

[0037] Example 2 is a further example based on Example 1, and the addition ratio of the liquid strains of Bacillus subtilis and Bacillus coagulans is 2:1.

Embodiment 3

[0038] Example 3 is a further example based on Example 1, the addition ratio of the liquid strains of Bacillus subtilis and Bacillus coagulans is 1:2.

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PUM

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Abstract

The invention discloses a method for Bacillus subtilis and Bacillus coagulans mixed fermentation, and a use method thereof. A traditional liquid individual fermentation culture technology is improved, solid mixed fermentation is combined, and different physiologic characteristics of Bacillus subtilis and Bacillus coagulans are combined, so a fermentation product increases the absorption and utilization of nutrient substances in bird animals and improves the disease resistance of the bird animals when used to feed the bird animals. The method concretely comprises: seed activation culture, strain enlarged culture, liquid deep fermentation, solid aerobic fermentation, low-temperature environmentally-friendly drying, and crushing and packaging.

Description

technical field [0001] The invention relates to the mechanical field, in particular to a method for mixed fermentation of bacillus subtilis and bacillus coagulans and its application. Background technique [0002] Bacillus subtilis ( Bacillus subtilis ), which is a mesophilic aerobic spore-forming rod-shaped bacterium (G 十 ), the spores are oval or columnar, mesogenic or partially mesogenic, even if the cysts are enlarged, it is not obvious; it is mostly used in aquatic products, livestock and poultry breeding, and the addition method is mostly used to make bacterial powder, but the price is more expensive. It is currently recognized as an excellent probiotic with development prospects. Bacillus subtilis, which is a kind of aerobic or facultative anaerobic Gram-positive bacteria, can grow and reproduce in the intestinal tract of animals, secrete and produce various enzymes (such as protease, lipase, amylase, etc.) and nutrient metabolism Products (such as a variety of ami...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18C12R1/125C12R1/07
CPCC12N1/20
Inventor 何小建
Owner 西安鑫汉宝生物科技有限公司
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