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Method for extracting polyphenol substance from defatted rice bran

A technology of degreased rice bran and extraction method, which is applied in the direction of drug combination, medical formula, plant raw materials, etc., can solve the problems of insoluble bound polyphenols that cannot be extracted, and the extraction effect is not ideal, so as to achieve high polyphenol extraction rate and active destruction Small, the effect of increasing the extraction rate of polyphenols

Inactive Publication Date: 2017-08-18
JIANGSU FOOD & PHARMA SCI COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult to extract the insoluble polyphenolic compounds formed by combining with cellulose and lipoproteins with general organic solvents.
[0003] From the current data analysis, most researchers directly use methanol, ethanol or acetone to extract polyphenols in grains. In order to improve the extraction yield, methods such as reducing particle size, prolonging extraction time, or improving extraction stability are often used. The method can only extract free polyphenols and soluble bound phenolic acids in grains, but cannot extract insoluble bound polyphenols, so the extraction effect is not ideal

Method used

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  • Method for extracting polyphenol substance from defatted rice bran
  • Method for extracting polyphenol substance from defatted rice bran
  • Method for extracting polyphenol substance from defatted rice bran

Examples

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Effect test

Embodiment 1

[0037] A method for extracting polyphenols in defatted rice bran, comprising the following steps:

[0038] Step 1, pretreatment: take fresh rice bran and put it in an oven at 100°C, take it out after 15 minutes and dry it in the air.

[0039] Step 2, degreasing: Accurately weigh 10g of crushed rice bran powder sample, soak in 200mL hexane for 60min, then add 200mL hexane to soak for 60min after suction filtration, repeat 3 times to remove the fat in rice bran, after suction filtration is completed Dry the filter residue in a constant temperature oven at 70°C for 4 hours;

[0040] Step 3, alkali hydrolysis: Weigh the dried degreased rice bran powder, put it into NaOH solution with a concentration of 0.5mol / L according to the ratio of solid to liquid at 1:5 to carry out the hydrolysis of the bound phenolic substances, and adjust the pH to 3.0;

[0041] Step 4, extraction: After mixing the hydrolyzed solution obtained in step 3 with 50% ethanol, put it into an ultrasonic instru...

Embodiment 2

[0045] A method for extracting polyphenols in defatted rice bran, comprising the following steps:

[0046] Step 1, pretreatment: take fresh rice bran and put it in an oven at 100°C, take it out after 15 minutes and dry it in the air, grind it with a universal grinder after drying, sieve it with 60 meshes, and store it in a freezer at -20°C;

[0047] Step 2, degreasing: Accurately weigh 10g of crushed rice bran powder sample, soak in 200mL hexane for 60min, then add 200mL hexane to soak for 60min after suction filtration, repeat 3 times to remove the fat in rice bran, after suction filtration is completed Dry the filter residue in a constant temperature oven at 70°C for 4 hours;

[0048] Step 3, alkali hydrolysis: Weigh the dried defatted rice bran powder, put it into NaOH solution with a concentration of 3.0mol / L according to the ratio of material to liquid of 1:9 to carry out the hydrolysis of the bound phenolic substances, and adjust the pH to 5.0;

[0049] Step 4, extract...

Embodiment 3

[0051] A method for extracting polyphenols in defatted rice bran, comprising the following steps:

[0052] Step 1, pretreatment: take fresh rice bran and put it in an oven at 100°C, take it out after 15 minutes and dry it in the air, grind it with a universal grinder after drying, sieve it with 60 meshes, and store it in a freezer at -20°C;

[0053] Step 2, degreasing: Accurately weigh 10g of crushed rice bran powder sample, soak in 200mL hexane for 60min, then add 200mL hexane to soak for 60min after suction filtration, repeat 3 times to remove the fat in rice bran, after suction filtration is completed Dry the filter residue in a constant temperature oven at 70°C for 4 hours;

[0054] Step 3, alkali hydrolysis: Weigh the dried defatted rice bran powder, put it into NaOH solution with a concentration of 2.5mol / L according to the ratio of solid to liquid at 1:8 to carry out hydrolysis of bound phenolic substances, and adjust the pH to 4.56;

[0055] Step 4, extraction: After m...

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Abstract

The invention discloses a method for extracting a polyphenol substance from defatted rice bran. The method comprises the steps of pretreatment, degreasing, basic hydrolysis and extraction, wherein the basic hydrolysis step comprises the sub-steps of weighing dried defatted rice bran, putting the defatted rice bran into a NaOH solution with the concentration of 0.5-3.0mol / L at the liquid-solid ratio of (1:5) to (1:9), carrying out phenolic substance-combined hydrolysis, hydrolyzing for 1-4h and adjusting the pH to be 3.0-5.0 by using HCL; and in the extraction process, hydrolysate obtained in the third step is extracted by using ethanol for 3-5 times, extracting solutions are mixed to be concentrated in vacuum and are completely extracted by using ethyl acetate, sodium sulfate is concentrated again after being dewatered and is dissolved by using methanol, and the volume is metered. The method for extracting the polyphenol substance from the defatted rice bran is high in polyphenol extraction yield, the damage to the activity of a polyphenol extract is small, the method can be applied to extraction and production of the polyphenol substance in the defatted rice bran, and a relatively good way is provided for resource development and application of the defatted rice bran.

Description

technical field [0001] The invention relates to a method for extracting polyphenols in defatted rice bran. Background technique [0002] Rice bran is a mixture of cortex peeled off during rice milling, rice germ and a small amount of broken rice. It is an inevitable by-product of rice processing, accounting for about 5-5.5% of the mass of rice grains. Modern epidemiological studies have shown that eating whole grains or related products has a good effect on reducing chronic diseases such as cardiovascular and cerebrovascular diseases, which is mainly due to the unique free polyphenols in whole grains. However, the content of pure free phenolic acids in grains is not much, and they are mainly combined with proteins and sugars through hydrogen bonds, hydrophobic interactions or covalent bonds. According to the tightness of binding, phenolic acids in rice can be divided into three types: free form, soluble binding form or insoluble binding form. Among them, free phenolic acids...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/899A61P9/10A61P9/00
Inventor 翟玮玮侯会绒孙兆远
Owner JIANGSU FOOD & PHARMA SCI COLLEGE
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