Method for improving glucose acid conversion rate of glutamic acid fermentation through adding trehalase

Abstract

The invention relates to a method for improving the glucose acid conversion rate of glutamic acid fermentation through adding trehalase. The trehalase is added in a later period in the glutamic acid fermentation process, wherein the adding time is 20-32h during a fermentation period, the temperature is required to be 30-40 DEG C during addition and the adding ratio is 0.1-100ppm. The fermentation operation condition before enzyme connection is invariable. According to the method, the glucose acid conversion rate of glutamic acid fermentation can be improved, the acid yield can be improved, and meanwhile, the consumption of sugar can be reduced. Due to the method, the utilization rate of the fed sugar in the later fermentation period is improved, the consumption of the fed sugar is reduced, meanwhile, the sugar concentration in a fermentation liquid is reduced, electro-crystallization such as post-extraction of glutamic acid is facilitated, and thus, the method plays a great role in improvement of the quality of a glutamic acid product.

Description

technical field [0001] The invention belongs to the technical field of corn deep processing, in particular to a method for increasing the sugar-acid conversion rate of glutamic acid fermentation by adding trehalase. Background technique [0002] In recent years, the domestic glutamic acid fermentation industry has continuously innovated in the fermentation process and equipment, and the fermentation level has been greatly improved. Domestic glutamic acid fermentation production mainly uses biotin-deficient strains as production strains and starch hydrolyzed glucose as carbon source. At present, glutamic acid thermosensitive strains are used, and high-concentration glucose solution is fed into the fermentation process. High acid production. The fed-batch process is an optimized form of the batch fermentation process. Its purpose is to avoid the inhibition of the initial concentration of the substrate and increase the input amount of the substrate. The feeding of high-concent...

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Problems solved by technology

[0003] In addition, as glutamic acid bacteria enter the late stage of fermentation, a large amount of trehalose will be accumulated during the metabolic process, and trehalose is a non-reducing sugar composed of two glucose molecules with 1,1-glycosidic bonds. This sugar cannot be absorbed by glutamic acid. Utilized by bacteria, it accumulates continuously in the TCA cycle, which has a very adverse effect on the normal metabolism of glutamic acid bacteria

At the same time, because the added glucose cannot be consumed in time through the normal metabolic pathway, on the one hand, the unnecessary consumption of sugar will be increased, and on the other hand, the actual sugar content in the fermentation broth will increase, which will have many adverse effects on the isoelectric crystallization of glutamic acid after extraction.

[0004] Through the search, no patent publications related to the patent application of the present invention have been found

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