Method for extracting protein from cottonseed meal

A technology of cottonseed meal and protein, applied in the field of protein extraction, can solve the problems of unsuitable large-scale industrial production, low efficiency and purity, cumbersome methods, etc., and achieve the effects of improving defects, simple production process, and saving water resources

Inactive Publication Date: 2017-08-18
合肥云都棉花有限公司
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Chinese patent CN101785527A "A method for preparing dephenolized cottonseed protein and raffinose" uses an organic solvent method for the extraction of protein and sugar in cottonseed meal. The gossypol is removed thoroughly, but it will also bring the organic solvent Into the protein and sugar, and its method is cumbersome, complex, efficiency and purity are not high, not suitable for large-scale industrial production, so there is a need for a better extraction method for the preparation of protein in cottonseed

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for extracting protein from cottonseed meal, comprising the steps of:

[0027] a. After pulverizing the cottonseed meal, mix it with water, the weight ratio of cottonseed meal to water is 1:15, and carry out centrifugation;

[0028] b. Filter the solution, remove the residue, then dissolve the filtrate in alkali, and add protease, the weight ratio of protease to filtrate is 1:20, heat the filtrate and stir, keep the temperature at 40°C, the pH value of the solution is 7.5, and the heating time At 5h, the stirring rate was 80r / min;

[0029] c. further washing the residue of step b with water, and then performing the operation of step b separately, and mixing the obtained filtrate with the filtrate of step b;

[0030] d. Rapidly raise the temperature of the above mixed filtrate to 80°C, sterilize and eliminate enzymes for 10 minutes, stop heating, and filter the residue again;

[0031] e. Adding a sodium chloride solution with a concentration of 8% to the filtr...

Embodiment 2

[0040] A method for extracting protein from cottonseed meal, comprising the steps of:

[0041] a. After pulverizing the cottonseed meal, mix it with water, the weight ratio of cottonseed meal to water is 1:15, and carry out centrifugation;

[0042] b. Filter the solution, remove the residue, then dissolve the filtrate in alkali, and add protease, the weight ratio of protease to filtrate is 1:20, heat the filtrate and stir, keep the temperature at 45 °C, the pH value of the solution is 7.5, and the heating time At 5h, the stirring rate was 68r / min;

[0043] c. further washing the residue of step b with water, and then performing the operation of step b separately, and mixing the obtained filtrate with the filtrate of step b;

[0044] d. Rapidly raise the temperature of the mixed filtrate to 89°C, sterilize and kill enzymes for 9 minutes, stop heating, and filter the residue again;

[0045] e. Adding a sodium chloride solution with a concentration of 8% to the filtrate, the we...

Embodiment 3

[0054] A method for extracting protein from cottonseed meal, comprising the steps of:

[0055] a. After pulverizing the cottonseed meal, mix it with water, the weight ratio of cottonseed meal to water is 1:15, and carry out centrifugation;

[0056] b. Filter the solution, remove the residue, then dissolve the filtrate in alkali, and add protease, the weight ratio of protease to filtrate is 1:20, heat the filtrate and stir, keep the temperature at 50°C, the pH value of the solution is 7.5, and the heating time At 5h, the stirring rate was 70r / min;

[0057] c. further washing the residue of step b with water, and then performing the operation of step b separately, and mixing the obtained filtrate with the filtrate of step b;

[0058] d. Rapidly raise the temperature of the mixed filtrate to 90°C, sterilize and kill enzymes for 5 minutes, stop heating, and filter the residue again;

[0059] e. Adding a sodium chloride solution with a concentration of 8% to the filtrate, the wei...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for extracting protein from cottonseed meal. The method comprises the following steps: a, adding water and mixing after smashing the cottonseed meal, and carrying out centrifugal separation; b, filtering a solution, removing residues, then carrying out alkali dissolution, adding protease, heating, and keeping the temperature be 40 to 50 DEG C, the pH (Potential of Hydrogen) value of the solution be 7.5 to 8.0 and the heating time be 3 to 5 hours; c, quickly rising the temperature to 80 to 90 DEG C, and stopping heating after carrying out sterilization and enzyme deactivation treatment; d, then adding sodium chloride in the solution, and concentrating and drying the solution after uniformly stirring, thus obtaining the protein in the cottonseed meal. According to the method provided by the invention, the resource is fully utilized, the protein which is high in nutritive value in the cottonseed meal is extracted, the extracted protein is high in purity and few in impurities and has no residue of an industrial extraction additive, a preparation technology is simple, the cost is low, but the value is high, and the method is suitable for industrial large-scale production.

Description

technical field [0001] The invention belongs to the technical field of cottonseed processing, and in particular relates to a method for extracting protein from cottonseed meal. Background technique [0002] my country is a large cotton-producing country, accounting for more than 60% of the world's total cotton output. In 2008, the cotton output was 20.526 million tons, and 11.49 million tons of cottonseed were produced. After oil extraction, 6.43 million tons of cottonseed meal were produced, which contained about 3.2 million tons of protein. In the past, about 5% of the cottonseed meal after cottonseed oil was used as animal feed, and more than 95% was used as fertilizer, wasting a lot of protein. [0003] The protein content in cottonseed is generally 14-17%, and the highest can reach 29.7%. Its potency is similar to that of animal protein, and it is a good protein. Cottonseed protein is rich in amino acid content, and the content of 18 kinds of amino acids is reasonable, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 程飞
Owner 合肥云都棉花有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap