Uses and kits of graphene quantum dots

A technology of graphene quantum dots and kits, applied in the field of fluorescence detection, can solve the problems of complex operation, many steps, high cost, etc., and achieve high sensitivity and strong specificity

Active Publication Date: 2019-10-08
CHONGQING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These techniques are complicated to operate, many steps, high cost, and due to the short life of superoxide anion radicals, the quantitative detection of the scavenging reaction of superoxide anion radicals based on SOD is easily affected by the concentration of superoxide anion radicals. The impact of inaccuracies

Method used

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  • Uses and kits of graphene quantum dots
  • Uses and kits of graphene quantum dots
  • Uses and kits of graphene quantum dots

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] This embodiment provides a kit for detecting superoxide dismutase, which includes: graphene quantum dots and sodium hypochlorite solution.

[0050] The present embodiment also provides a detection method for superoxide dismutase, which comprises the following steps:

[0051] a. Prepare sodium hypochlorite solution (1mM) and standard solutions containing superoxide dismutase (concentrations of superoxide dismutase are 1U / mL, 3U / mL, 5U / mL, 20U / mL, respectively).

[0052] b. Mix standard solutions of different concentrations with sodium hypochlorite solution and graphene quantum dots to obtain a plurality of first mixed solutions; at the same time, uniformly mix sodium hypochlorite solution and graphene quantum dots to obtain a blank control solution.

[0053] c. Set the excitation wavelength to 365nm, respectively measure the fluorescence emission spectra of the blank control solution and multiple first mixed solutions, and measure the fluorescence intensity at the maximu...

Embodiment 2

[0057] This embodiment provides a kit for detecting superoxide dismutase, which includes: graphene quantum dots and sodium hypochlorite solution.

[0058] Wherein, the preparation method of graphene quantum dot comprises:

[0059] Take 2g of citric acid and heat it to 200°C. After 5 minutes, the citric acid liquefies; then, the color of the solution changes from colorless to light yellow, and then turns orange after 30 minutes. Stop heating.

[0060] Add the obtained orange solution dropwise to 100mL, 10mg / mL of NaOH, stir, and adjust the pH to 7 to obtain graphene quantum dots. figure 2 shown.

experiment example 1

[0062] This experimental example illustrates the use of a hypochlorite ion provided in the embodiment of the present invention in quenching the fluorescence generated by graphene quantum dots.

[0063] Sodium hypochlorite solution and graphene quantum dots were mixed evenly, as the experimental group, placed under a 365nm ultraviolet lamp for observation, and the graphene quantum dot solution was used as the control group, the results were as follows image 3 shown in the illustration.

[0064] In the control group, graphene quantum dots are yellow-green under 365nm ultraviolet light (such as image 3 shown in the middle a tube solution); and in the experimental group, the mixed solution was colorless under the 365nm ultraviolet lamp (as image 3 As shown in the b tube solution), it can be seen that hypochlorite ions have a fluorescence quenching effect on graphene quantum dots.

[0065] The excitation wavelength was set to 365nm, and the fluorescence emission spectra of the...

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Abstract

The invention provides an application of a graphene quantum dot and a kit and belongs to the field of fluorescence detection. The application of the graphene quantum dot comprises an application of hypochlorite ions in quenching fluorescence generated by the graphene quantum dot, an application of hypochlorite ions in preventing fluorescent interference of the graphene quantum dot, an application of the graphene quantum dot in detecting superoxide dismutase, and an application of the graphene quantum dot in preparing a detection kit of superoxide dismutase. The invention also provides a preparation method of the superoxide dismutase. The preparation method comprises the following step of mixing the graphene quantum dot, a hypochlorite solution with a to-be-tested solution. Through studies of the inventor, fluorescence generated by the graphene quantum dot can be quenched by the hypochlorite ions, thereby providing a novel concept and policy for wide application of the graphene quantum dot and accurate detection of the graphene quantum dot in a later period.

Description

technical field [0001] The invention relates to the field of fluorescence detection, in particular to a use and kit of graphene quantum dots. Background technique [0002] Superoxide dismutase (SOD) is widely distributed in organisms and is an important substance for scavenging free radicals in organisms. The level of superoxide dismutase in organisms is closely related to aging and death. It can resist and block the damage to cells caused by oxygen free radicals, and repair damaged cells in time to repair the cell damage caused by free radicals. Therefore, detecting the concentration of superoxide dismutase in biological samples is of great significance in clinical medicine and health. [0003] Existing methods for detecting superoxide dismutase activity include cytochrome C reduction method, pyrogallol method, hydroxylamine method, immunological method and so on. Taking the pyrogallol method as an example, pyrogallol undergoes autoxidation reaction in a buffer solution ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 胡连哲刘光娟刘蕴付文升
Owner CHONGQING NORMAL UNIVERSITY
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