Analysis method of identifying black locust honey and syrup adulterated black locust honey

Abstract

The invention discloses an analysis method of identifying black locust honey and syrup adulterated black locust honey, and particularly relates to a method of combining an ultra-high performance liquid chromatography-quadrupole rod-orbitrap high resolution mass spectrum technique and metabonomics. The analysis method comprises the following steps: respectively pre-processing a genuine black locust honey sample and syrup adulterated black locust honey to be detected by adopting an organic solvent, realizing the separation and determination of chemical components of the preprocessed sample by applying an ultra-high performance liquid chromatography-quadrupole rod-orbitrap high resolution mass spectrum method, then preprocessing the obtained UHPLC-MS original data of the genuine black locust honey sample and the black locust honey sample to be detected, and finally analyzing main components by using a multivariate statistical analysis method so as to identify the black locust honey and the syrup adulterated black locust honey. The analysis method has the beneficial effects that the black locust honey and the syrup adulterated black locust honey are comprehensively analyzed by multivariate statistical analysis after the information of metabolites of the black locust honey and the syrup adulterated black locust honey are comprehensively obtained, thereby completing the detection of the syrup adulterated black locust honey.

Description

technical field [0001] The invention belongs to the technical field of food adulteration identification, and in particular relates to an analysis method for identifying acacia honey and syrup adulterated acacia honey by using a metabolomics technology combined with UHPLC-Q Exactive. Background technique [0002] Honey is a natural sweet substance that bees collect from the nectar, secretion or honeydew of plants, mix it with their own secretions, and fully brew. Honey has high nutritional value, and its main components are fructose and glucose. 70%, in addition to sugar substances, there are also minerals, proteins, amino acids, enzymes and vitamins in honey, which is an ideal natural food for human beings. Taking honey regularly has a good auxiliary medical effect on heart disease, high blood pressure, lung disease, eye disease, liver disease, dysentery, constipation, anemia, nervous system diseases, gastric and duodenal ulcer diseases, etc. External use can also treat bur...

AI Technical Summary

Problems solved by technology

The sensitivity of nuclear magnetic resonance (NMR) is low, and the metabolites we are concerned about in honey may be ignored, and NMR equipment is expensive and not suitable for daily monitoring

Near-infrared spectroscopy (NIRS) also has its fatal shortcomings: 1. It requires a large number of representative samples with known chemical values ​​to build models

Thus, the analysis of small batches of samples using near-infrared becomes impractical

2. The model needs to be updated continuously. Due to the change of the instrument state or the change of the standard sample, the model will also change accordingly.

3. The model is not universal, and the model of each instrument is different, which increases the limitation of use

4. The cost of modeling is high and the cost of testing is high

The various syrups currently adulterated with honey are very similar to honey in terms of physical and chemical properties, composition and content, and flavor. Therefore, the above-mentioned methods for measuring the quality of honey cannot detect honey adulterated with syrup

[0007] To sum up, the existing technology lacks a fast, effective and intuitive method for identifying the adulteration of acacia honey, and there is no combination of ultra-high performance liquid chromatography-quadrupole-orbitrap high-resolution mass spectrometry combined with metabolomics Studies on Determination of Marker Metabolites in Acacia Honey by Scientific Method

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