Analysis method of identifying black locust honey and syrup adulterated black locust honey

An analysis method, the technology of acacia honey, which is applied in the field of food adulteration identification, can solve the problems of high modeling cost, increased use limitations, and non-universal models, and achieve low operational technical requirements, accurate and reliable detection results, and simple processing methods quick effect

Active Publication Date: 2017-08-29
SHANDONG INST FOR FOOD & DRUG CONTROL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The sensitivity of nuclear magnetic resonance (NMR) is low, and the metabolites we are concerned about in honey may be ignored, and NMR equipment is expensive and not suitable for daily monitoring
Near-infrared spectroscopy (NIRS) also has its fatal shortcomings: 1. It requires a large number of representative samples with known chemical values ​​to build models
Thus, the analysis of small batches of samples using near-infrared becomes impractical
2. The model needs to be updated continuously. Due to the change of the instrument state or the change of the standard sample, the model will also change accordingly.
3. The model is not universal, and the model of each instrument is different, which increases the limitation o

Method used

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  • Analysis method of identifying black locust honey and syrup adulterated black locust honey
  • Analysis method of identifying black locust honey and syrup adulterated black locust honey
  • Analysis method of identifying black locust honey and syrup adulterated black locust honey

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Effect test

Embodiment 1

[0077] (1) method principle of the present invention:

[0078] After the real acacia honey sample and the syrup adulterated acacia honey sample were treated with the same pretreatment method, UHPLC was used in series with Thermo Fisher Q Exactive Mass Spectrometer to realize the separation and determination of the chemical components in the sample, and the Compounds Discoverer software was used to obtain the original The data were extracted from chromatographic peaks, peak alignment and library search analysis of unknown compounds. PCA analysis was used to distinguish true acacia honey from syrup adulterated acacia honey.

[0079] (2) Sample pretreatment

[0080] Weigh 1g acacia honey sample (the number of repetitions for one sample is 6 times) in a 50mL centrifuge tube, add 20mL extractant (the extractant is mixed with equal volumes of methanol containing 1% formic acid and ultrapure water), and vortex mix until the acacia The honey sample was completely dissolved, and the ...

Embodiment 2

[0095] The method for screening the marker metabolites of acacia honey based on UHPLC tandem Thermo Fisher Q Exactive Mass Spectrometer combined with metabolomics method comprises the following steps:

[0096] (1) Sample pretreatment

[0097] Put 1g acacia honey sample in a 50mL centrifuge tube, add 20mL extractant (the extractant is mixed with equal volumes of methanol containing 1% formic acid and ultrapure water), vortex and mix until the acacia honey sample is completely dissolved, and place the centrifuge tube in ultrasonic Sonicate in a washing machine for 25 minutes, centrifuge at 1000 rpm for 5 minutes, and filter the supernatant with a 0.22 μm organic filter membrane for sample loading.

[0098] Put 1g of syrup sample in a 50mL centrifuge tube, add 20mL of extractant (the extractant is mixed with equal volumes of methanol containing 1% formic acid and ultrapure water), vortex and mix until the syrup sample is completely dissolved, and place the centrifuge tube in an u...

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Abstract

The invention discloses an analysis method of identifying black locust honey and syrup adulterated black locust honey, and particularly relates to a method of combining an ultra-high performance liquid chromatography-quadrupole rod-orbitrap high resolution mass spectrum technique and metabonomics. The analysis method comprises the following steps: respectively pre-processing a genuine black locust honey sample and syrup adulterated black locust honey to be detected by adopting an organic solvent, realizing the separation and determination of chemical components of the preprocessed sample by applying an ultra-high performance liquid chromatography-quadrupole rod-orbitrap high resolution mass spectrum method, then preprocessing the obtained UHPLC-MS original data of the genuine black locust honey sample and the black locust honey sample to be detected, and finally analyzing main components by using a multivariate statistical analysis method so as to identify the black locust honey and the syrup adulterated black locust honey. The analysis method has the beneficial effects that the black locust honey and the syrup adulterated black locust honey are comprehensively analyzed by multivariate statistical analysis after the information of metabolites of the black locust honey and the syrup adulterated black locust honey are comprehensively obtained, thereby completing the detection of the syrup adulterated black locust honey.

Description

technical field [0001] The invention belongs to the technical field of food adulteration identification, and in particular relates to an analysis method for identifying acacia honey and syrup adulterated acacia honey by using a metabolomics technology combined with UHPLC-Q Exactive. Background technique [0002] Honey is a natural sweet substance that bees collect from the nectar, secretion or honeydew of plants, mix it with their own secretions, and fully brew. Honey has high nutritional value, and its main components are fructose and glucose. 70%, in addition to sugar substances, there are also minerals, proteins, amino acids, enzymes and vitamins in honey, which is an ideal natural food for human beings. Taking honey regularly has a good auxiliary medical effect on heart disease, high blood pressure, lung disease, eye disease, liver disease, dysentery, constipation, anemia, nervous system diseases, gastric and duodenal ulcer diseases, etc. External use can also treat bur...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 宿书芳耿越祝建华江瑶王骏王凯利
Owner SHANDONG INST FOR FOOD & DRUG CONTROL
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