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Gnaphalium affine extract and preparation method and application thereof

A technology of koji grass and extract, applied in the field of koji grass extract, can solve the problem of low xanthine oxidase inhibitory activity, and achieve the effects of low cost of medicinal materials, huge development potential and abundant wild resources

Inactive Publication Date: 2017-09-05
SHANGHAI INST OF PHARMA IND CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome the low xanthine oxidase inhibitory activity of the Salmonella extract in the prior art, and provide a Salmonella extract, its preparation method and application

Method used

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  • Gnaphalium affine extract and preparation method and application thereof
  • Gnaphalium affine extract and preparation method and application thereof
  • Gnaphalium affine extract and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0031] After drying in the shade, cut Sagegrass into sections, weigh 5kg, mix with 75L (1:15, kg / L) of 60% (v / v) ethanol aqueous solution, heat and reflux for extraction 4 times, each time for 2h, and combine the extracts. The extract was concentrated under reduced pressure (60°C) to 5 L (Sagemus : Concentrate volume 1:1, kg / L) to obtain the extract concentrate.

[0032] Extract the concentrated solution by adding 60-90°C petroleum ether for extraction three times, each time the volume ratio of the concentrated solution to petroleum ether is 1:1 (v / v), discard the petroleum ether phase to obtain the aqueous phase. Ethyl acetate was added to the aqueous phase, and extracted three times, each time the volume ratio of the aqueous phase to ethyl acetate was 1:1 (v / v). After extraction, the ethyl acetate phase was combined, and reduced to dryness to obtain 102.3 g.

[0033] The prepared sage extract (96g) was separated by 200-300 mesh silica gel column chromatography (specificatio...

Embodiment 2

[0046] After drying in the shade, cut Sageweed into sections, weigh 0.6kg, mix with 50% (v / v) ethanol aqueous solution 6L (1:10, kg / L), heat and reflux for extraction three times, each time for 2 hours, and combine the extracts. The extract was concentrated under reduced pressure (60°C) to 1.2L (Sagemusia : Concentrate volume 1:2, kg / L) to obtain the extract concentrate.

[0047] Extract the concentrated solution by adding petroleum ether at 60-90°C to extract twice, each time the volume ratio of the concentrated solution to petroleum ether is 1:2 (v / v), discard the petroleum ether phase, and obtain the aqueous phase. Ethyl acetate was added to the aqueous phase, and extracted twice, each time the volume ratio of the aqueous phase to ethyl acetate was 1:2 (v / v). After extraction, the ethyl acetate phase was combined, and reduced to dryness to obtain 11.7 g.

Embodiment 3

[0049] After drying in the shade, cut Sageweed into sections, weigh 2kg, mix with 40L (1:20, kg / L) of 80% (v / v) ethanol aqueous solution, heat and reflux for extraction twice, each time for 2h, and combine the extracts. The extract was concentrated under reduced pressure (60°C) to 1 L (Sagemus : concentrate volume 1:0.5, kg / L) to obtain the extract concentrate.

[0050] Extract the concentrated solution by adding petroleum ether at 60-90°C to extract 4 times, each time the volume ratio of the concentrated solution to petroleum ether is 1:1 (v / v), discard the petroleum ether phase, and obtain the aqueous phase. Ethyl acetate was added to the aqueous phase, and extracted 4 times, each time the volume ratio of the aqueous phase to ethyl acetate was 1:1 (v / v). After extraction, the ethyl acetate phase was combined, and reduced to dryness to obtain 42.3 g.

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Abstract

The invention discloses a gnaphalium affine extract and a preparation method and application thereof. The preparation method of the gnaphalium affine extract comprises the following steps: mixing gnaphalium affine with ethanol water, extracting and concentrating to obtain a concentrated solution; extracting the concentrated solution with petroleum ether to obtain a water phase; and extracting the water phase with ethyl acetate, and collecting an ethyl acetate phase to obtain the final product. The volume percentage of the ethanol water is 50-80%. The gnaphalium affine extract has high xanthine oxidase inhibition activity; and gnaphalium affine wild resources are abundant, the cost of medicinal materials is low, and the developing potential is huge.

Description

technical field [0001] The present invention relates to an extract of sage japonicus, its preparation method and application. Background technique [0002] Uric acid is the final metabolite of human purine compounds, and disorders of purine metabolism can lead to hyperuricemia. Hyperuricemia (HUA) refers to the level of blood uric acid on an empty stomach that is higher than 420 μmol / L for men and higher than 360 μmol / L for women under a normal purine diet. Hyperuricemia is caused by increased production or decreased excretion of uric acid in the body. Hyperuricemia is the basis of gout. When the concentration of uric acid is supersaturated to form crystals and deposit in the joint synovium, soft tissue, cartilage and kidneys, it will cause "gout", which will trigger the body's innate immune response and cause joint and surrounding pain. Tissue inflammation and renal impairment. In patients with hyperuricemia, the metabolic function of sugar and fat in the body will be si...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28C07C67/48C07C67/56C07C69/732C07C69/757A61K31/216A61P19/06A23L33/00
CPCA23V2002/00A61K31/216A61K36/28A61K2236/333A61K2236/35A61K2236/55C07C67/48C07C67/56C07C69/732C07C69/757A23V2200/30A23V2250/21
Inventor 范思洋吴春珍张伟
Owner SHANGHAI INST OF PHARMA IND CO LTD
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