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Method for extracting coumarin compound from kidney beans

A technology of coumarins and compounds, which is applied in the fields of pharmaceutical formulations, organic chemistry, plant raw materials, etc., can solve the problems of increased yield and low purity of coumarins, and achieve the effects of promoting precipitation, improving purity, and increasing yield

Inactive Publication Date: 2017-09-05
于世金
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Technical problem to be solved: In order to solve the technical problem of low purity of coumarin in the existing extraction method, the present invention uses magnesium sulfate, sodium citrate and nicotinamide to heat and extract effective substances, which can not only greatly improve the concentration of coumarin compounds Purity can also increase the yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for extracting coumarin compounds from kidney beans, comprising the following steps:

[0023] Step 1, take the kidney beans, add the seeds to 70 times the weight of water after peeling, and beat to obtain a slurry;

[0024] Step 2, in parts by weight, add 4 parts of magnesium sulfate, 1 part of sodium citrate and 0.5 part of nicotinamide to 100 parts of the obtained slurry, heat to 70°C for extraction for 4 hours, and filter through a microfiltration membrane after cooling to obtain the extract;

[0025] In step 3, the extract obtained in step 2 is introduced into an ion exchange resin for adsorption, and then eluted with a 3wt.% sodium chloride solution to obtain an eluent;

[0026] Step 4, concentrating the eluate obtained in step 3 with a nanofiltration membrane to obtain a concentrated solution;

[0027] Step 5, evaporating and concentrating the concentrated solution in step 4 until the volume is reduced to 10% of the original volume, adding ethanol aqueou...

Embodiment 2

[0032] A method for extracting coumarin compounds from kidney beans, comprising the following steps:

[0033] Step 1, take the kidney beans, add the seeds to 75 times the weight of water after peeling, and beat to obtain a slurry;

[0034] Step 2: In parts by weight, 6 parts of magnesium sulfate, 2 parts of sodium citrate and 0.7 parts of nicotinamide were added to 100 parts of the obtained slurry, heated to 75°C for extraction for 4 hours, cooled and filtered through a microfiltration membrane to obtain an extract;

[0035] In step 3, the extract obtained in step 2 is introduced into an ion exchange resin for adsorption, and then eluted with 4wt.% sodium chloride solution to obtain an eluent;

[0036] Step 4, concentrating the eluate obtained in step 3 with a nanofiltration membrane to obtain a concentrated solution;

[0037] Step 5, evaporating and concentrating the concentrated solution in step 4 until the volume is reduced to 15% of the original volume, adding ethanol aqu...

Embodiment 3

[0042] A method for extracting coumarin compounds from kidney beans, comprising the following steps:

[0043] Step 1, take the kidney beans, add the seeds to 90 times the weight of water after peeling, and beat to obtain a slurry;

[0044] Step 2: In parts by weight, 8 parts of magnesium sulfate, 3 parts of sodium citrate and 0.9 parts of nicotinamide were added to 100 parts of the obtained slurry, heated to 80°C for extraction for 4 hours, cooled and filtered through a microfiltration membrane to obtain an extract;

[0045] In step 3, the extract obtained in step 2 is introduced into an ion exchange resin for adsorption, and then eluted with a 3wt.% sodium chloride solution to obtain an eluent;

[0046] Step 4, concentrating the eluate obtained in step 3 with a nanofiltration membrane to obtain a concentrated solution;

[0047] Step 5, evaporating and concentrating the concentrated solution in step 4 until the volume is reduced to 15% of the original volume, adding ethanol a...

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Abstract

The invention provides a method for extracting a coumarin compound from kidney beans. The method comprises the following steps: grinding kidney bean seeds into paste and adding magnesium sulfate, sodium citrate and niacinamide; heating and extracting to separate out more effective substances from the paste; absorbing and eluting an extracting solution through ion exchange resin to remove impurities; concentrating by adopting a nano-filtration membrane and extracting a concentrated solution with an ethanol water solution; finally, concentrating and crystallizing an extracting solution to obtain the coumarin compound. According to the extraction method provided by the invention, the purity of the coumarin compound can be extremely improved; cellulase is added in an extracting process and the effective substances can also be separated out, so that the yield of the coumarin compound is improved.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, and in particular relates to a method for extracting coumarin compounds from kidney beans. Background technique [0002] The scientific name of kidney beans is Kidney Bean (commonly known as Kidney Bean or Kidney Bean), belonging to the genus Kidney Bean of the Fabaceae family. Kidney beans are native to Mexico and Argentina in the Americas. The pods are 10-20 cm long, straight or slightly curved in shape, round or oblate in cross-section, and the epidermis is densely covered with fluff; the young pods are in different shades of green, yellow, purple (or streaked) and other colors, yellow and white when mature to tan. With the development of pods, the vascular bundles at the dorsal and ventral sutures gradually developed, and the number of sclerenchyma layers in the mesocarp and endocarp gradually increased, and the quality of fresh food decreased. Therefore, it is necessa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48C07G99/00C07D311/02
CPCA61K36/48C07D311/02A61K2236/15A61K2236/331A61K2236/333C07G99/00
Inventor 于世金
Owner 于世金
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