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Method of simultaneous saccharification total fermentation

A simultaneous saccharification fermentation and simultaneous saccharification technology, applied in fermentation, biochemical equipment and methods, and microbial-based methods, can solve the problems of low xylose utilization, high production costs of in situ enzymes, and low lignocellulose conversion efficiency. problems, to achieve the effect of improving conversion efficiency, improving utilization rate, and high alcohol concentration

Inactive Publication Date: 2017-09-08
TIANJIN UNIV OF SCI & TECH
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Problems solved by technology

[0005] Aiming at the disadvantages of high production cost of in situ enzymes, low xylose utilization rate, and low conversion efficiency of lignocellulose to ethanol, the present invention adopts a synchronous saccharification and co-fermentation method of two bacteria, Saccharomyces cerevisiae and Fusarium oxysporum

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  • Method of simultaneous saccharification total fermentation

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Effect test

Embodiment 1

[0019] (1) Cut the naturally air-dried wheat straw into small pieces with a length of less than 3cm, add 0.5mol / L NaOH solution according to the ratio of material to liquid 1:20 (g:mL), mix well, and place in a high-pressure steam sterilizer at 121°C 1. Pretreatment for 1 hour, washing the solid residue with water until neutral, drying and pulverizing.

[0020] (2) Pre-enzymolyze the above-mentioned material treated with NaOH, the reaction volume is 100 mL, and the dextran loading is 3% (w / v). Pre-enzymatic hydrolysis for 12-24 hours. Cellulase and β-glucosidase were added to the enzymatic hydrolysis system at an enzyme load of 25 FPU / g dextran, and xylanase was added to the enzyme system at a load of 10 mg / g dextran. Ampickanamycin with a final concentration of 50 mg / L was added to prevent bacterial contamination during the fermentation process.

[0021] (3) The enzymatic hydrolysis condition is 50°C, the rotating speed of the shaker is 180 rpm, and 1 mL is taken after 12 h...

Embodiment 2

[0025] (1) Cut the naturally air-dried wheat straw into small pieces with a length of less than 3cm, add 0.5mol / L NaOH solution according to the ratio of material to liquid 1:20 (g:mL), mix well, and place in a high-pressure steam sterilizer at 121°C 1. Pretreatment for 1 hour, washing the solid residue with water until neutral, drying and pulverizing.

[0026] (2) Pre-enzymolyze the above-mentioned material treated with NaOH, the reaction volume is 100 mL, and the dextran loading is 3% (w / v). Pre-enzymatic hydrolysis for 12-24 hours. Cellulase and β-glucosidase were added to the enzymatic hydrolysis system at an enzyme load of 25 FPU / g dextran, and xylanase was added to the enzyme system at a load of 10 mg / g dextran. Ampickanamycin with a final concentration of 50 mg / L was added to prevent bacterial contamination during the fermentation process.

[0027] (3) The enzymatic hydrolysis condition is 50°C, the rotating speed of the shaker is 180 rpm, and 1 mL is taken after 12 h...

Embodiment 3

[0031] (1) Cut the naturally air-dried wheat straw into small pieces with a length of less than 3cm, add 0.5mol / L NaOH solution according to the ratio of material to liquid 1:20 (g:mL), mix well, and place in a high-pressure steam sterilizer at 121°C 1. Pretreatment for 1 hour, washing the solid residue with water until neutral, drying and pulverizing.

[0032] (2) Pre-enzymolyze the above-mentioned material treated with NaOH, the reaction volume is 100 mL, and the dextran loading is 3% (w / v). Pre-enzymatic hydrolysis for 12-24 hours. Cellulase and β-glucosidase were added to the enzymatic hydrolysis system at an enzyme load of 25 FPU / g dextran, and xylanase was added to the enzyme system at a load of 10 mg / g dextran. Ampickanamycin with a final concentration of 50 mg / L was added to prevent bacterial contamination during the fermentation process.

[0033] (3) The enzymatic hydrolysis condition is 50°C, the rotating speed of the shaker is 180pm, and 1 mL is taken after 12 hou...

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Abstract

The invention addresses the shortcomings of high production cost of in-situ enzyme, low utilization rate of xylose and low conversion efficiency from lignocellulose to ethanol, and adopts a method of simultaneous saccharification total fermentation by utilizing two strains of saccharomyces cerevisiae and fusarium oxysporum. Before the simultaneous saccharification fermentation, pretreatment of straw raw materials is conducted by using NaOH solution. By accretion of a small amount of commercial cellulase enzyme, pre-enzymatic hydrolysis of raw material is conducted, then by accretion of fusarium oxysporum and saccharomyces cerevisiae at different time periods, the antagonistic actions of the two strains of bacteria are prevented, the utilization rate of hexoses and pentoses is improved, so that the aim of producing ethanol by using lignocellulose is achieved.

Description

technical field [0001] The invention belongs to the field of lignocellulose degradation and microbial metabolism, and mainly relates to a method for synchronous saccharification and co-fermentation of two bacteria, Saccharomyces cerevisiae and Fusarium oxysporum, to produce ethanol by adding Fusarium oxysporum and Saccharomyces cerevisiae in different time periods The antagonism of the two bacteria is avoided, and the utilization rate of hexose and pentose is improved, so as to achieve the purpose of using lignocellulose to produce ethanol, and at the same time reduce the total cost through the production of in situ enzymes. Background technique [0002] Cellulose is the most widespread type of carbohydrate in nature, and it is also the most abundant biomass resource on the earth. Plants produce about 1.55×10 11 tons of cellulose materials, and the amount of crop straw in my country alone reaches more than 700 million tons. At present, only a small part of cellulose has bee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P7/10C12P7/14C12R1/865C12R1/77
CPCC12P7/10C12P7/14C12P39/00Y02E50/10
Inventor 钟成舒月力贾士儒李栋梁侯颖谭之磊韩培培吕和鑫
Owner TIANJIN UNIV OF SCI & TECH
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