A method for in vitro preservation and restoration of purple ginseng potato germplasm

Abstract

The invention discloses a dioscorea alata L. germplasm in-vitro preservation and restoration method. The method comprises the following steps: cutting and potting the middle of healthy and disease-free dioscorea alata L. rhizomes, performing sand culture so as to obtain seedlings, performing disinfection treatment on seedling stems serving as explants, inoculating in a solid medium so as to obtain sterile test-tube plantlets; cutting stems of the sterile test-tube plantlets and inoculating in a conservative medium: 1 / 3-1 / 2MS+2.0mg / L KT+0.2mg / L NAA+20-30mg / L of mannitol+60mg / L of saccharose+7mg / L of agar+0.1% of AC, the pH value is equal to 5.8. The test-tube plantlets can be healthily preserved for 16 months or longer under normal temperature conditions, and the survival rate reaches 85% or higher; compared with the conventional subculture seedlings, the method in the invention has the advantages that the plant root is strong and developed, nodes are obviously shortened, and the seedlings are small in leaves, high in tillering and deep in leaf color. After the subculture test-tube plantlets restore growth, the plant growth is normal, and the test-tube plantlets do not have any difference from the conventional tissue culture seedlings.

Description

technical field [0001] The invention relates to the technical field of plant germplasm resource preservation, in particular to a method for in vitro preservation and recovery of purple ginseng and potato germplasm. Background technique [0002] Purple ginseng potato (Dioscorea alata.L) is a twining herbaceous vine of Dioscorea genus, also known as purple ginseng and purple yam. It is widely cultivated in Zhejiang, Jiangxi, Fujian, Taiwan, Guangdong and other provinces in southern my country. The tubers are oval to oblate, with purple-brown skin and bright purple flesh. They are rich in protein, vitamins, dioscin, anthocyanins and other nutrients. Immunity and other effects, it is one of the crops with both medicine and food. Purple ginseng potato is a vegetatively propagated crop, which does not produce zero seeds under normal conditions in the field, and is mainly propagated by tubers. At present, the preservation of purple ginseng potato germplasm resources mainly adopts f...

AI Technical Summary

Problems solved by technology

At present, the germplasm resources of purple ginseng and potato are mainly preserved by field tuber propagation and preservation. This method is time-consuming and laborious, and is easily affected by various natural disasters, diseases and insect pests, resulting in loss of germplasm, short preservation time and poor preservation effect.

Use general tissue culture technology to preserve the germplasm resources of purple ginseng potato. The test tube seedlings need to be subcultured regularly (transfer once every 1-2 months), and the continuous repetition of work will cause a lot of waste of manpower and material resources. At the same time, the chance of contamination may also be improve

The use of low temperature or ultra-low temperature storage technology, the operation procedures are more cumbersome, the equipment cost is large, and the energy consumption is high.

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