A method for in vitro preservation and restoration of purple ginseng potato germplasm

A technology for in vitro preservation and purple ginseng potato, which is applied in the field of plant germplasm resource preservation, can solve the problems of high equipment cost, the impact of natural disasters, diseases and insect pests, and short storage time, so as to save manpower and material resources, facilitate germplasm exchange, long-lasting effect

Active Publication Date: 2019-02-19
MINNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the germplasm resources of purple ginseng and potato are mainly preserved by field tuber propagation and preservation. This method is time-consuming and laborious, and is easily affected by various natural disasters, diseases and insect pests, resulting in loss of germplasm, short preservation time and poor preservation effect.
Use general tissue culture technology to preserve the germplasm resources of purple ginseng potato. The test tube seedlings need to be subcultured regularly (transfer once every 1-2 months), and the continuous repetition of work will cause a lot of waste of manpower and material resources. At the same time, the chance of contamination may also be improve
The use of low temperature or ultra-low temperature storage technology, the operation procedures are more cumbersome, the equipment cost is large, and the energy consumption is high.

Method used

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  • A method for in vitro preservation and restoration of purple ginseng potato germplasm
  • A method for in vitro preservation and restoration of purple ginseng potato germplasm
  • A method for in vitro preservation and restoration of purple ginseng potato germplasm

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Embodiment 1

[0018] A method for in vitro preservation and restoration of purple ginseng potato germplasm, the specific steps of which are as follows:

[0019] 1. Obtaining and disinfection of explants

[0020] Take healthy, disease-free purple ginseng potato tubers and cut them into potted sand cultures to obtain purple ginseng potato seedlings. Cut out the jointed tender stems of the seedlings and put them in a beaker. Rinse them with running water for 2 hours, soak them in a concentrated solution of washing powder for 15 minutes, and rinse them with tap water. , transpose it on the ultra-clean workbench and soak it in 70% alcohol for 30s, transfer it to 2% sodium hypochlorite solution and soak it for 15 minutes, then rinse it with sterile water for 3 times, each time for 2 minutes; after washing with sterile water, get The sterilized purple ginseng potato tender stem explant;

[0021] 2. Obtaining sterile vaccines:

[0022] Put the sterilized tender stems in step 1 on the ultra-clean ...

Embodiment 2

[0029] 1. Obtaining and disinfection of explants

[0030] Take healthy and disease-free purple ginseng potato tubers, cut them into pieces, and cultivate them in potted sand to obtain purple ginseng potato seedlings. Cut the jointed tender stems of the seedlings and put them in a beaker. After washing with running water for 1 hour, wash them with a concentrated solution of washing powder for 20 minutes, and rinse them with tap water. Soak in 70% alcohol for 45 seconds on a transposed ultra-clean workbench, transfer to 2% sodium hypochlorite solution and soak for 10 minutes, then rinse with sterile water for 4 times, each time for 2 minutes; obtain disinfection after washing with sterile water explants of purple ginseng and potato tender stems;

[0031] 2. Obtaining sterile vaccines:

[0032]Put the sterilized tender stems in step 1 on the ultra-clean workbench, cut them into single-segmented stems, about 2cm in length, and cut off excess parts such as petioles and branches, a...

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Abstract

The invention discloses a dioscorea alata L. germplasm in-vitro preservation and restoration method. The method comprises the following steps: cutting and potting the middle of healthy and disease-free dioscorea alata L. rhizomes, performing sand culture so as to obtain seedlings, performing disinfection treatment on seedling stems serving as explants, inoculating in a solid medium so as to obtain sterile test-tube plantlets; cutting stems of the sterile test-tube plantlets and inoculating in a conservative medium: 1 / 3-1 / 2MS+2.0mg / L KT+0.2mg / L NAA+20-30mg / L of mannitol+60mg / L of saccharose+7mg / L of agar+0.1% of AC, the pH value is equal to 5.8. The test-tube plantlets can be healthily preserved for 16 months or longer under normal temperature conditions, and the survival rate reaches 85% or higher; compared with the conventional subculture seedlings, the method in the invention has the advantages that the plant root is strong and developed, nodes are obviously shortened, and the seedlings are small in leaves, high in tillering and deep in leaf color. After the subculture test-tube plantlets restore growth, the plant growth is normal, and the test-tube plantlets do not have any difference from the conventional tissue culture seedlings.

Description

technical field [0001] The invention relates to the technical field of plant germplasm resource preservation, in particular to a method for in vitro preservation and recovery of purple ginseng and potato germplasm. Background technique [0002] Purple ginseng potato (Dioscorea alata.L) is a twining herbaceous vine of Dioscorea genus, also known as purple ginseng and purple yam. It is widely cultivated in Zhejiang, Jiangxi, Fujian, Taiwan, Guangdong and other provinces in southern my country. The tubers are oval to oblate, with purple-brown skin and bright purple flesh. They are rich in protein, vitamins, dioscin, anthocyanins and other nutrients. Immunity and other effects, it is one of the crops with both medicine and food. Purple ginseng potato is a vegetatively propagated crop, which does not produce zero seeds under normal conditions in the field, and is mainly propagated by tubers. At present, the preservation of purple ginseng potato germplasm resources mainly adopts f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 蔡月琴陆銮眉
Owner MINNAN NORMAL UNIV
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