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Culture medium for promoting growth of morchella mycelia

A technology for mycelial growth and culture medium, applied in the field of promoting the growth of Morchella hyphae, can solve problems such as insufficient supply of Morchella hyphae, and achieve the effects of low price, wide source and cost reduction.

Inactive Publication Date: 2017-09-15
张云辉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the defect of existing culture medium formula, provide a kind of method that can promote the growth of hickory chick hyphae, to solve the defect of insufficient supply of hickory chick mycelium, improve the output of hickory chick polysaccharide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The optimization test of embodiment 1 carbon source, nitrogen source

[0015] Carbon source: add 2% glucose, fructose, maltose, sucrose, soluble starch, corn flour, and flour to the basal medium as carbon sources to form seven different carbon source culture media, and use the basal medium as a carbon-free source The control group was used to study the effects of different carbon sources on the growth of Morchella mycelium.

[0016] Studies have found that maltose is the most suitable carbon source for morel bacteria. The effect of maltose as carbon source is the best, the mycelial growth rate is 2,89mm / d, the mycelial diameter is 42.59mm, and the mycelial growth index is 13.50. From the analysis of mycelial growth index, the colony diameter of maltose was significantly increased compared with the control group.

[0017] Nitrogen source: Add 0.3% peptone, yeast powder, soybean powder, ammonium tartrate, ammonium sulfate, and ammonium nitrate as nitrogen sources to the...

Embodiment 2

[0020] A culture medium for promoting the growth of morel mycelia, the formula of which is as follows: maltose 16g / L; ammonium tartrate 0.25g / L; KH2PO40.8g / L; MgSO4•7H2O0.3g / L; VB18mg / L; agar 18g / L.

[0021] The configuration method is: weigh maltose, ammonium tartrate, KH2PO4, MgSO4•7H2O, VB1, dissolve in 1L of water and add, stir well, add agar and heat and stir until the agar melts; put it into a triangular flask, and use Seal with parafilm and sterilize at 121°C for 30 minutes for later use.

[0022] Morel culture:

[0023] (1) Inoculation: When the temperature of the culture medium drops to 36°C, inoculate morels, seal them with a parafilm and place them in an incubator for cultivation;

[0024] (2) Mycelia growth: Morchella was grown on the above-mentioned medium for 10 days in total, and the line was drawn on the third day after inoculation, and then lined every 24 hours for a total of 6 times; at the same time, observe the color and thickness of the mycelia , densit...

Embodiment 3

[0026] A culture medium for promoting the growth of morel mycelia, the formula of which is as follows: maltose 18g / L; ammonium tartrate 0.28g / L; KH2PO41.0g / L; MgSO4•7H2O0.4g / L; VB110mg / L; agar 20gg / L.

[0027] The configuration method is: weigh maltose, ammonium tartrate, KH2PO4, MgSO4•7H2O, VB1, dissolve in 1L of water and add, stir well, add agar and heat and stir until the agar melts; put it into a triangular flask, and use Seal with parafilm and sterilize at 121°C for 30 minutes for later use.

[0028] Morel culture:

[0029] (1) Inoculation: When the temperature of the culture medium drops to 38°C, inoculate morels, seal them with a parafilm and place them in an incubator for cultivation;

[0030] (2) Mycelia growth: Morchella was grown on the above-mentioned medium for 10 days in total, and the line was drawn on the third day after inoculation, and then lined every 24 hours for a total of 6 times; at the same time, observe the color and thickness of the mycelia , dens...

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Abstract

The invention provides a culture medium for promoting the growth of morchella mycelia. For the culture medium, maltose is adopted as an optimal carbon source for culturing the morchella mycelia, ammonium tartrate is adopted as an optimal nitrogen source for culturing the morchella mycelia, high-purity chemical reagents with accurate ingredients are selected for preparation, so that the ingredients of the culture medium are accurate, and the quality control of the strains is facilitated. According to the nutrition ingredients required by the growth of the morchella mycelia, optimization is conducted, so that the growth speed of the morchella mycelia is accelerated, after inoculation is carried out for 7-9 days, the morchella mycelia grow on the whole inclined plane, the growth speed of the mycelia is improved by about 20% compared with the growth speed of the mycelia adopting a PDA culture medium, and the growth time of the morchella mycelia is shortened, in addition, the culture medium disclosed by the invention is simple in formula, wide in raw material source and low in cost, the production cost of the morchella can be reduced, and reliable technical support is provided for subsequent application of the morchella.

Description

technical field [0001] The invention relates to the field of cultivation of edible and medicinal fungi, in particular to a method for promoting the growth of hickory chick hyphae by optimizing the culture medium. Background technique [0002] Morchella is a precious perennial large medicinal fungus, which gets its name because it mostly grows on Morus plants and its fruiting bodies are yellowish brown. The medicinal effect of hickory chick was first seen in "Compendium of Materia Medica" and "On the Properties of Medicine" to record that hickory chick tastes bitter and cold in nature. In traditional Chinese medicine in my country, it is used to treat symptoms such as dysentery, night sweats, metrorrhagia, and prolapse of the anus. The latest research shows that Morchella also has a unique anti-cancer effect, and is currently a medicinal fungus with the highest efficiency among internationally recognized biological cancer treatment agents. The main pharmacological active in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 张云辉
Owner 张云辉
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