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Lactic acid bacteria composite microbial agent and preparation method and application thereof

A compound bacterial agent, lactic acid bacteria technology, applied in the directions of biochemical equipment and methods, applications, microorganism-based methods, etc., can solve the problems of long fermentation time, single effect, low yield, etc., and achieve improved survival rate, stable quality and cost. low effect

Active Publication Date: 2017-09-15
广州市微生物研究所集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the defects of the above single microecological preparations such as single action, low output, and long fermentation time, and provide a lactic acid bacteria composite bacterial agent. The lactic acid bacteria composite bacterial agent provided by the present invention has high yield and short fermentation time, and can be applied to lactic acid bacteria. In solid beverage or lactic acid bacteria feed additive

Method used

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  • Lactic acid bacteria composite microbial agent and preparation method and application thereof
  • Lactic acid bacteria composite microbial agent and preparation method and application thereof
  • Lactic acid bacteria composite microbial agent and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Embodiment 1 The preparation of Lactobacillus reuteri freeze-dried bacterial powder

[0040] 1. Liquid submerged fermentation culture

[0041] Inoculate 2% inoculum of Lactobacillus reuteri into 150mL seed medium in a 250mL Erlenmeyer flask at 37°C, 5% CO 2 cultured in an incubator. After 24 h, inoculate 800 mL seed medium in a 1 L Erlenmeyer flask with 3% inoculum amount, and inoculate at 37 °C, 5% CO 2 cultured in an incubator. After culturing for about 7 hours, inoculate into a 200mL fermenter with a 3% inoculum size, and contain 160mL fermentation medium.

[0042] The fermentation culture conditions are: temperature 37°C, rotation speed 50rpm, pressure 0.05MPa, ventilation rate 1.5L / H, during the fermentation process, the pH value is controlled between 5.5 and 6.5 with 30% potassium hydroxide solution, and the OD600 value of the fermentation broth and After the pH value became stable, the tank was put into the tank, and the culture time was about 8 hours. When t...

Embodiment 2

[0049] The preparation of embodiment 2 Lactobacillus plantarum freeze-dried bacteria powder

[0050] 1. Liquid submerged fermentation culture

[0051] Inoculate 2% inoculum of Lactobacillus plantarum into 150mL seed medium in a 250mL Erlenmeyer flask, at 37°C, 5% CO 2 cultured in an incubator. After 24 h, inoculate 800 mL seed medium in a 1 L Erlenmeyer flask with 3% inoculum amount, and inoculate at 37 °C, 5% CO 2 cultured in an incubator. After culturing for about 7 hours, inoculate into a 200mL fermenter with a 3% inoculum size, and contain 160mL fermentation medium.

[0052] The fermentation conditions are as follows: temperature 37°C, rotation speed 80rpm, pressure 0.05MPa, ventilation rate 0 L / H, the initial pH value of the fermentation broth is 5.8, and the pH value is controlled at about 5.8 with ammonia water during the fermentation process, with the OD600 value and pH of the fermentation broth After the value became stable, the tank was put into the tank, and the...

Embodiment 3

[0059] Embodiment 3 The preparation of Lactobacillus rhamnosus freeze-dried bacterial powder

[0060] 1. Liquid submerged fermentation culture

[0061] Inoculate 2% inoculum of Lactobacillus rhamnosus into 150mL seed medium in a 250mL Erlenmeyer flask and store at 37°C in 5% CO 2 cultured in an incubator. After 24 h, inoculate 800 mL seed medium in a 1 L Erlenmeyer flask with 3% inoculum amount, and inoculate at 37 °C, 5% CO 2 cultured in an incubator. After culturing for about 7 hours, inoculate into a 200mL fermenter with a 3% inoculum size, and contain 160mL fermentation medium.

[0062] The fermentation conditions are as follows: temperature 37°C, rotation speed 80rpm, pressure 0.05MPa, ventilation rate 0 L / H, the initial pH value of the fermentation broth is 5.8, and the pH value is controlled at about 5.8 with ammonia water during the fermentation process, with the OD600 value and pH of the fermentation broth After the value became stable, the tank was put into the t...

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Abstract

The invention relates to a lactic acid bacteria composite microbial agent and a preparation method and application thereof, the lactic acid bacteria composite microbial agent includes Lactobacillus reuteri, Lactobacillus plantarum and Lactobacillus rhamnosus, the preparation method of the lactic acid bacteria composite microbial agent is as follows: respective submerged liquid fermentation of the Lactobacillus reuteri, the Lactobacillus plantarum and the Lactobacillus rhamnosus in a liquid, centrifugal collection of bacteria mud, and vacuum freeze-drying to obtain lyophilized compound bacterial powder, namely the lactic acid bacteria composite microbial agent. The lactic acid bacteria composite microbial agent has high yield and short fermentation time, and can be applied to lactic acid bacteria solid beverages or lactic acid bacteria feed additives. The preparation method of the lactic acid bacteria composite microbial agent has the advantages of simple process, short fermentation time, high yield, low cost, suitability for industrial production, and wide popularization and application value.

Description

technical field [0001] The invention belongs to the field of microecological preparations, and more specifically relates to a lactic acid bacteria composite bacterial preparation and its preparation method and application. Background technique [0002] Microecological preparation is a kind of intestinal flora regulator, which has multiple functions such as preventing and treating animal diseases, regulating immune function, and improving the breeding environment. The probiotics currently used in probiotics mainly include lactic acid bacteria, bacillus and yeast. Lactic acid bacteria are the dominant flora in the microecological flora of human and animal digestive tracts. Studies have shown that lactic acid bacteria can produce biologically active substances such as organic acids, hydrogen peroxide, and bacteriocins, which can inhibit the growth of animal intestinal pathogens, promote digestion and absorption, maintain intestinal microecological balance, and enhance immune f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L2/38A23L2/52A23K10/18C12R1/225C12R1/25
CPCA23K10/18A23L2/38A23L2/52C12N1/20
Inventor 曹丁黄乐天张宜靖甘祥武李学优吕善进黄秀敏黄魁英夏枫耿
Owner 广州市微生物研究所集团股份有限公司
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