Preparation method of chelated calcium capable of accelerating development of skeletal cells
A technology of chelating calcium and cells, which is applied in the field of organic calcium, can solve the problems of low utilization rate and inability to promote the growth and development of bone cells, and achieve the effects of promoting proliferation and growth, improving local microcirculation, and improving the proliferation environment
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example 1
[0017] First select fresh leaves of Eucommia ulmoides and Panax notoginseng leaves, wash and dry naturally, mix and grind the leaves of Panax notoginseng and Eucommia ulmoides leaves at a mass ratio of 1:5, and pass through a 150-mesh sieve to obtain mixed ball-milled particles, at a mass ratio of 1:10 , mix the mixed ball-milled particles with deionized water, and heat in a water bath at 55°C for 2h; after the heating in the water bath is completed, centrifuge at 2500r / min for 10min, collect the supernatant and place it at 45°C for rotary evaporation to the original volume 1 / 5, to obtain the concentrated solution, according to the volume ratio of 1:8, the concentrated solution was mixed with absolute ethanol, stirred and mixed at room temperature for 1 hour, left to age for 6 hours, filtered to obtain the filtrate, and the filtrate was rotated at 45°C Evaporate to dryness to obtain a dry extract; select 1g of dry extract, 0.1mL Tween-80, and 85mL DMEM cell culture solution in ...
example 2
[0020] First select fresh leaves of Eucommia ulmoides and Panax notoginseng leaves, wash and dry naturally, mix and grind the leaves of Panax notoginseng and Eucommia ulmoides leaves at a mass ratio of 1:5, and pass through a 150-mesh sieve to obtain mixed ball-milled particles, at a mass ratio of 1:10 , mix the mixed ball-milled particles with deionized water, and heat in a water bath at 58°C for 3h; after the heating in the water bath is completed, centrifuge at 2800r / min for 13min, collect the supernatant and put it in a rotary evaporation at 48°C to the original volume 1 / 5, to obtain the concentrated solution, according to the volume ratio of 1:8, the concentrated solution was mixed with absolute ethanol, stirred and mixed at room temperature for 2 hours, left to age for 7 hours, filtered to obtain the filtrate, and the filtrate was rotated at 48°C Evaporate to dryness to obtain a dry extract; select 2g of dry extract, 0.2mL Tween-80, and 93mL DMEM cell culture solution in ...
example 3
[0023] First select fresh leaves of Eucommia ulmoides and Panax notoginseng leaves, wash and dry naturally, mix and grind the leaves of Panax notoginseng and Eucommia ulmoides leaves at a mass ratio of 1:5, and pass through a 150-mesh sieve to obtain mixed ball-milled particles, at a mass ratio of 1:10 , mix the mixed ball-milled particles with deionized water, and heat in a water bath at 60°C for 3h; after the heating in the water bath is completed, centrifuge at 3000r / min for 15min, collect the supernatant and place it at 50°C for rotary evaporation to the original volume 1 / 5, to obtain the concentrated solution, according to the volume ratio of 1:8, the concentrated solution was mixed with absolute ethanol, stirred and mixed at room temperature for 2 hours, left to age for 8 hours, filtered to obtain the filtrate, and the filtrate was rotated at 50°C Evaporate to dryness to obtain a dry extract; select 2g of dry extract, 0.2mL Tween-80, and 100mL DMEM cell culture solution in ...
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