A strain of Acinetobacter and its application in petroleum degradation
A bacillus and petroleum technology, applied in the field of Acinetobacter and its application in petroleum-contaminated soil or water, can solve the problems of increased number of harmful spores and pests, breeding of molds and pests, environmental pollution, etc., and achieve low price and high growth Short cycle, good treatment effect
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Embodiment 1A
[0044] The acquisition of embodiment 1 Acinetobacter baylyi LHK bacterial strain
[0045] 1. Screening, isolation and purification of petroleum degrading bacteria
[0046] (1) Source of the strain: The oil-degrading bacteria was isolated from oil-contaminated soil in Liaohe Oilfield.
[0047] (2) Enrichment of strains:
[0048] a. Take 5 g of oil-polluted sludge samples, add them to diesel inorganic salt medium containing 1% diesel oil, and culture them at a constant temperature in a shaking incubator at 30° C. for 5 days at a shaking frequency of 170 rpm.
[0049] b. Take 5 mL of the supernatant from the culture solution in the previous step, transfer it to a new diesel inorganic salt medium containing 1% diesel oil, and culture it in a shaking incubator at 30°C for 5 days at a shaking frequency of 170rpm.
[0050] c. Repeat step b four times until the solution in the diesel inorganic salt medium is relatively clear.
[0051] (3) Purification of bacterial strains
[0052]...
Embodiment 2A
[0060] Low temperature resistance, salt resistance, alkali resistance of embodiment 2Acinetobacter baylyi LHK
[0061] 1. Effect of temperature (°C) on the growth of strain LHK
[0062] Using a single factor variable, an equal amount of bacterial strain LHK was inoculated into diesel inorganic salt liquid medium (pH = 7.0, salinity 0%) under the same conditions, at 25°C, 30°C, and 37°C, respectively. After culturing for 6 days, samples were taken every 24 hours to determine the OD 600nm The absorption value is used to measure the size of its growth. The study found that the growth of strain LHK at low temperature of 25°C and 30°C was better than that of 37°C, such as Figure 4 shown.
[0063] 2. Effect of initial pH on the growth of strain LHK
[0064] Using single factor variables, an equal amount of bacterial strain LHK bacterial liquid was inoculated into diesel inorganic salt liquid medium (pH=7.0, salinity 0%) under the same conditions, and the initial pH was adjusted...
Embodiment 3A
[0067] The water body remediation test of embodiment 3Acinetobacter baylyi LHK
[0068] The strain LHK was inoculated into the inorganic salt medium containing 1% diesel oil according to the inoculum amount of 1%, and cultured in a constant temperature shaking shaker at 30°C and 170rpm for 7 days, and then the degradation efficiency of diesel oil was determined by ultraviolet spectrophotometry. The results are shown in Table 2.
[0069] Table 2 Degradation efficiency of bacterial strain LHK to diesel oil in inorganic salt medium under different conditions
[0070]
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