A kind of preparation method of separating medium

A technology for separating media and macromolecules, applied in the field of preparation of separation media, can solve problems such as limited application of mechanical strength, and achieve good biological compatibility, excellent mechanical properties, and good cross-linking effect.

Active Publication Date: 2019-11-19
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since the low mechanical strength of conventional hydrogels greatly limits their application in material separation, how to prepare porous hydrogel separation media with good mechanical properties has been a meaningful and challenging problem.

Method used

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  • A kind of preparation method of separating medium
  • A kind of preparation method of separating medium
  • A kind of preparation method of separating medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Preparation of Porous Hydrogel Separation Medium

[0029] Step 1: Dissolve BSA in 10 mL ultrapure water to obtain a BSA solution with a concentration of 100 mg / mL, and add 1% cross-linking agent β-diimine zinc complex and 1,2,7, 1 mL each of 8-dioxoctane, then add 20 mg / mL NaCl 2 mL, mix well, and adjust the pH of the solution to 2.6 with dilute hydrochloric acid;

[0030] Step 2: Divide the mixed solution in Step 1 into molds, and keep at 40°C for 3 hours to prepare a hydrogel;

[0031] Step 3: Transfer the hydrogel in step 2 into a high-pressure steam sterilizer and keep it at 121°C for 20 minutes, soak and wash it in distilled water for 5 days, and change the washing water every 6 hours to remove salt and residual crosslinking agent;

[0032] Step 4: Pre-freeze the washed gel in Step 3 at -80°C for 3 h and then vacuum freeze-dry to obtain a porous hydrogel separation medium.

[0033] figure 2 For the SEM image of the hydrogel separation medium prepared ...

Embodiment 2

[0034] Example 2 Preparation of Porous Hydrogel Separation Medium

[0035] Step 1: Dissolve HSA in 10 mL ultrapure water to obtain a HSA solution with a concentration of 150 mg / mL, and add 1% cross-linking agent β-diimine zinc complex and 1,2,7, 1 mL each of 8-dioxoctane, then add 2 mL of 50 mg / mL KCl, mix well, and adjust the pH of the solution to 3.5 with dilute hydrochloric acid;

[0036] Step 2: Divide the mixed solution in Step 1 into molds, and keep at 50°C for 2 hours to prepare a hydrogel;

[0037] Step 3: Transfer the hydrogel in step 2 into a high-pressure steam sterilizer and keep it at 115°C for 30 minutes, soak and wash it in distilled water for 5 days, and change the washing water every 6 hours to remove salt and residual crosslinking agent;

[0038] Step 4: Pre-freeze the washed gel in Step 3 at -80°C for 3 h and then vacuum freeze-dry to obtain a porous hydrogel separation medium.

[0039] The physical and chemical properties of the porous hydrosol obtained i...

Embodiment 3

[0040] Example 3 Preparation of Porous Hydrogel Separation Medium

[0041] Step 1: Dissolve LHC in 10 mL ultrapure water to obtain an LHC solution with a concentration of 100 mg / mL, and add 2% cross-linking agent β-diimine zinc complex and 1,2,7, 1 mL each of 8-dioxoctane, then add 100 mg / mL NaNO 3 Solution 2mL, mix well, and adjust the pH of the solution to 5 with dilute hydrochloric acid;

[0042]Step 2: Divide the mixed solution in Step 1 into molds, and keep it at 60°C for 2 hours to prepare a hydrogel;

[0043] Step 3: Put the primary gel in step 2 into a high-pressure steam sterilizer and keep it at 121°C for 15 minutes, soak and wash in distilled water for 5 days, and change the washing water every 6 hours to remove salt and residual crosslinking agent;

[0044] Step 4: drying the washed gel in step 3 by supercritical carbon dioxide method to obtain a porous hydrogel separation medium.

[0045] The physical and chemical properties of the porous hydrosol obtained in t...

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Abstract

The invention relates to a preparation method of a novel separation medium. First, a certain amount of inorganic salt and composite crosslinking agent β-diimine zinc complex and 1,2,7,8-diepoxide are added to a macromolecular protein solution octane, adjust the pH of the solution to 2-5.5, stir well and then heat in a water bath to prepare a hydrogel. The prepared hydrogel is subjected to high temperature treatment at 110-121° C., soaked and washed in distilled water to remove the residual inorganic salt and monomer crosslinking agent, and then dried to obtain a high-strength porous hydrogel. The hydrogel has good elasticity and strong compression resistance, the maximum compressive strain and compressive stress can reach 70% and 5‑6MPa, respectively, the porosity can be controlled at 75‑90%, and it has good selective separation ability. Separation media are used in the field of separation and purification.

Description

technical field [0001] The invention relates to a preparation method of a separation medium, in particular to a preparation method of a gel type separation medium. Background technique [0002] Hydrogel is a water-rich polymer with a three-dimensional network structure, which is generally used in the fields of tissue engineering and drug sustained release. Although hydrogels often have controllable porosity, they are often expected to be used for the separation of substances. However, since the low mechanical strength of conventional hydrogels greatly limits their application in material separation, how to prepare porous hydrogel separation media with good mechanical properties has been a meaningful and challenging problem. [0003] The present invention aims to prepare a superporous hydrogel. The hydrogel can overcome the defects of conventional porous hydrogels, has a stable pore structure, high swelling rate, and good mechanical properties, and can be used as a separati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/24C08J3/075C08J9/12B01D17/02B01J20/26B01J20/30C08L89/00
CPCB01D17/0202B01J20/26C08J3/075C08J3/24C08J9/12C08J2389/00
Inventor 惠俊峰范代娣米钰郑晓燕邓建军姜西娟
Owner NORTHWEST UNIV
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