Adult-stem-cell adipogenesis inducing medium
A technology for inducing culture medium and adult stem cells, applied in the field of stem cells, can solve the problems of troublesome operation, long time for adipogenic induction of stem cell culture medium, low efficiency of stem cell adipogenic induction, etc., and achieves the effect of convenient use and high efficiency
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Embodiment 1
[0025] Such as Figure 1-5 As shown, a kind of adult stem cell adipogenic induction medium of the present invention, described medium comprises basal medium, also comprises following component and its concentration: IBMX 56ug / ml, dexamethasone 8umol / ml, insulin 6umol / ml, Testosterone 7umol / ml, indomethacin 689umol / ml;
[0026] The basal medium includes components at the following concentrations: alanine 47mg / l, glutamic acid 38mg / l, arginine 354mg / l, aspartic acid 41mg / l, asparagine 67mg / l, cysteine Amino acid 41mg / l, Glutamine 230mg / l, Glycine 32mg / l, Histidine 38mg / l, Hydroxyproline 37mg / l, Isoleucine 37mg / l, Leucine 77mg / l, Lysine Acid 67mg / l, Methionine 42mg / l, Phenylalanine 41mg / l, Proline 42mg / l, Serine 37mg / l, Threonine 42mg / l, Tryptophan 38mg / l, Disodium Tyrosine 67mg / l, valine 39mg / l, calcium chloride 225mg / l, magnesium sulfate 71mg / l, potassium chloride 210mg / l, potassium dihydrogen phosphate 32mg / l, disodium hydrogen phosphate 1180mg / l, sodium chloride 0.4 g / l, ...
Embodiment 2
[0029] The culture medium includes basal culture medium, and also includes the following components and their concentrations: IBMX 2ug / ml, dexamethasone 0.2umol / ml, insulin 0.4umol / ml, testosterone 0.08umol / ml, indomethacin 18umol / ml;
[0030]The basal medium includes components at the following concentrations: alanine 47mg / l, glutamic acid 38mg / l, arginine 354mg / l, aspartic acid 41mg / l, asparagine 67mg / l, cysteine Amino acid 41mg / l, Glutamine 230mg / l, Glycine 32mg / l, Histidine 38mg / l, Hydroxyproline 37mg / l, Isoleucine 37mg / l, Leucine 77mg / l, Lysine Acid 67mg / l, Methionine 42mg / l, Phenylalanine 41mg / l, Proline 42mg / l, Serine 37mg / l, Threonine 42mg / l, Tryptophan 38mg / l, Disodium Tyrosine 67mg / l, valine 39mg / l, calcium chloride 225mg / l, magnesium sulfate 71mg / l, potassium chloride 210mg / l, potassium dihydrogen phosphate 32mg / l, disodium hydrogen phosphate 1180mg / l, sodium chloride 0.4 g / l, biotin 0.2mg / l, choline chloride 8mg / l, inositol 42mg / l, nicotinamide 3mg / l, D-pantothen...
Embodiment 3
[0032] The medium includes basic medium, and also includes the following components and their concentrations: IBMX 280ug / ml, dexamethasone 38umol / ml, insulin 39umol / ml, testosterone 37umol / ml, indomethacin 1820umol / ml.
[0033] The basal medium includes components at the following concentrations: alanine 47mg / l, glutamic acid 38mg / l, arginine 354mg / l, aspartic acid 41mg / l, asparagine 67mg / l, cysteine Amino acid 41mg / l, Glutamine 230mg / l, Glycine 32mg / l, Histidine 38mg / l, Hydroxyproline 37mg / l, Isoleucine 37mg / l, Leucine 77mg / l, Lysine Acid 67mg / l, Methionine 42mg / l, Phenylalanine 41mg / l, Proline 42mg / l, Serine 37mg / l, Threonine 42mg / l, Tryptophan 38mg / l, Disodium Tyrosine 67mg / l, valine 39mg / l, calcium chloride 225mg / l, magnesium sulfate 71mg / l, potassium chloride 210mg / l, potassium dihydrogen phosphate 32mg / l, disodium hydrogen phosphate 1180mg / l, sodium chloride 0.4 g / l, biotin 0.2mg / l, choline chloride 8mg / l, inositol 42mg / l, nicotinamide 3mg / l, D-pantothenic acid hemical...
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