A rapid detection device for sweat lactic acid

A detection device and lactic acid technology, which is applied in the field of medical detection, can solve the problems of unsatisfactory instant detection of lactic acid content changes, poor accuracy of chemical methods, and pain of the tester, and achieve good electron mobility, avoid trauma, and improve sensitivity.

Active Publication Date: 2019-06-25
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, chemical and enzymatic methods are commonly used for the determination of lactic acid. The accuracy of the chemical method is poor, the operation is complicated, and the detection efficiency is low; the enzymatic method includes the dehydrogenase method and the oxidase method, and the dehydrogenase method includes the ultraviolet method. There are also many problems with the colorimetric method coupled with tetrazolium salts. For example, lactate dehydrogenase is used to oxidize lactate to pyruvate while NAD is reduced to NADH. It is necessary to couple ALT and glutamic acid to remove the substrate pyruvate. Accelerate the reaction, or couple PMS and NBT with colorimetric determination, but the reaction has no end point, and it is difficult to judge; the oxidase method has high sensitivity and high accuracy, but the problem with this method is that it requires a large blood biochemical analyzer Or ultraviolet spectrophotometer and other special devices need to collect fingertip blood to measure lactic acid content. The equipment cost is high, the detection time is long, and it will bring pain to the tester. The detection is extremely inconvenient and cannot satisfy people's immediate detection of lactic acid content in the body. changing needs

Method used

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  • A rapid detection device for sweat lactic acid
  • A rapid detection device for sweat lactic acid

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Experimental program
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Effect test

preparation Embodiment 2

[0033] Cut Sargassum into small sections, soak and clean it repeatedly, dry under reduced pressure until the water content is below 5%, and crush it to a micron particle size of 80 μm to 150 μm by airflow; weigh 40 g of crushed Sargassum and add 0.8 L of it to a concentration of 0.5mol / L (C 2 h 3 0 2 ) 2 In the Ni solution, stir at room temperature for 9 hours, filter and dry the filter residue, add the activator sodium hydroxide and mix evenly, then add 1.2 times the volume of deionized water for 6 minutes, soak at room temperature for 14 hours, filter and dry under reduced pressure to obtain Residue: Put the residue into a horizontal tube furnace, pass in argon gas, slowly heat up to 950°C for 1.0h at a rate of 10°C per minute, cool and take it out; add nitric acid solution for cleaning, then add deionized water for cleaning, Dry to obtain graphene.

[0034] Weigh 4g nano TiO 2 Dissolved in deionized water, configured into a solution with a mass concentration of 0.25g / m...

preparation Embodiment 3

[0037] Cut Sargassum into small sections, soak and clean it repeatedly, dry under reduced pressure until the water content is below 5%, and then crush it to a micron particle size of 80 μm to 150 μm by airflow; weigh 60 g of crushed Sargassum and add 1.2 L of it to a concentration of 0.5mol / L (C 2 h 3 0 2 ) 2 In the Ni solution, stir at room temperature for 9 hours, filter and dry the filter residue, add the activator potassium carbonate and mix evenly, then add 1.2 times the volume of deionized water for 8 minutes, soak at room temperature for 13 hours, filter and dry under reduced pressure to obtain the residue ;Put the residue into a horizontal tube furnace, pass in argon, slowly heat up to 900°C for 1.3h at a rate of 8°C per minute, and take it out after cooling; add phosphoric acid solution for cleaning, then add deionized water for cleaning, and dry Dry to obtain graphene.

[0038] Weigh 4g nano TiO 2 Dissolved in deionized water, configured into a solution with a m...

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Abstract

The invention relates to the technical field of medical detection and particularly relates to a sweat lactic acid rapid detection device. The device comprises a flexible substrate layer, an electrode layer, an isolation layer and an electronically monitoring layer. The flexible substrate layer is made of a porous flexible material. The electrode layer comprises a counter electrode, a work electrode, a reference electrode and a reaction chamber. The work electrode comprises a second carbon paste lead, a carbon paste electrode III and a reaction electrode. The reaction electrode comprises an oxidase layer and a three-dimensional graphene composite material layer. The three-dimensional graphene composite material layer is prepared from nanometer TiO2 uniformly dispersed on graphene. The three-dimensional graphene composite material layer has a hollow microsphere network structure having pore size distribution of 20nm-40nm and a specific surface area of 1500 m<2> / g-1650 m<2> / g. The isolation layer is bonded to an insulation layer. The electronically monitoring layer comprises a voltage control unit, a current measurement unit and a data finishing unit. The detection device is free of blood sampling, can continuously monitor and detect the amount of human lactic acid and has the characteristics of high detection sensitivity, strong stability and carrying convenience.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a sweat lactic acid rapid detection device. Background technique [0002] Fatigue is a very complex physiological and psychological phenomenon, which is not composed of a single, clear factor, and the current definitions of fatigue are also very different. Generally speaking, fatigue is the result of labor, which is the decrease in the function of the whole body due to long-term intense mental or physical activities after a worker has worked continuously for a period of time. From a biomedical theory, labor is a process of energy consumption. When this process continues to a certain extent, the central nervous system will have an inhibitory effect. After the fatigue of the central nervous system, it is the fatigue of the motor nervous system, which reflects the sensitivity of the movement. Reduced, work efficiency decreased. During long-term intensive exercise, the mu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/30
CPCG01N27/308G01N27/3272G01N27/3277G01N27/3278
Inventor 糜漫天易龙朱俊东陈卡张乾勇
Owner ARMY MEDICAL UNIV
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