A rapid detection device for sweat lactic acid

Abstract

The invention relates to the technical field of medical detection and particularly relates to a sweat lactic acid rapid detection device. The device comprises a flexible substrate layer, an electrode layer, an isolation layer and an electronically monitoring layer. The flexible substrate layer is made of a porous flexible material. The electrode layer comprises a counter electrode, a work electrode, a reference electrode and a reaction chamber. The work electrode comprises a second carbon paste lead, a carbon paste electrode III and a reaction electrode. The reaction electrode comprises an oxidase layer and a three-dimensional graphene composite material layer. The three-dimensional graphene composite material layer is prepared from nanometer TiO2 uniformly dispersed on graphene. The three-dimensional graphene composite material layer has a hollow microsphere network structure having pore size distribution of 20nm-40nm and a specific surface area of 1500 m<2> / g-1650 m<2> / g. The isolation layer is bonded to an insulation layer. The electronically monitoring layer comprises a voltage control unit, a current measurement unit and a data finishing unit. The detection device is free of blood sampling, can continuously monitor and detect the amount of human lactic acid and has the characteristics of high detection sensitivity, strong stability and carrying convenience.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a sweat lactic acid rapid detection device. Background technique [0002] Fatigue is a very complex physiological and psychological phenomenon, which is not composed of a single, clear factor, and the current definitions of fatigue are also very different. Generally speaking, fatigue is the result of labor, which is the decrease in the function of the whole body due to long-term intense mental or physical activities after a worker has worked continuously for a period of time. From a biomedical theory, labor is a process of energy consumption. When this process continues to a certain extent, the central nervous system will have an inhibitory effect. After the fatigue of the central nervous system, it is the fatigue of the motor nervous system, which reflects the sensitivity of the movement. Reduced, work efficiency decreased. During long-term intensive exercise, the mu...

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Problems solved by technology

[0004] At present, chemical and enzymatic methods are commonly used for the determination of lactic acid. The accuracy of the chemical method is poor, the operation is complicated, and the detection efficiency is low; the enzymatic method includes the dehydrogenase method and the oxidase method, and the dehydrogenase method includes the ultraviolet method. There are also many problems with the colorimetric method coupled with tetrazolium salts. For example, lactate dehydrogenase is used to oxidize lactate to pyruvate while NAD is reduced to NADH. It is necessary to couple ALT and glutamic acid to remove the substrate pyruvate. Accelerate the reaction, or couple PMS and NBT with colorimetric determination, but the reaction has no end point, and it is difficult to judge; the oxidase method has high sensitivity and high accuracy, but the problem with this method is that it requires a large blood biochemical analyzer Or ultraviolet spectrophotometer and other special devices need to collect fingertip blood to measure lactic acid content. The equipment cost is high, the detection time is long, and it will bring pain to the tester. The detection is extremely inconvenient and cannot satisfy people's immediate detection of lactic acid content in the body. changing needs

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