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Method for preparing cryoprecipitate coagulation factor

A blood coagulation factor and cryoprecipitation technology, which is applied in blood diseases, medical formulas, extracellular fluid diseases, etc., can solve the problems of cryoprecipitate coagulation factors flowing into empty bags, failing to succeed, and taking a long time to achieve a high content of factor VIII , good clinical infusion effect and short preparation time

Inactive Publication Date: 2017-10-24
侯广安 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Disadvantages of refrigerator melting centrifugation method: this method takes too long, and factor VIII will have a certain attenuation
[0009] Disadvantages of the siphon method: This method requires special personnel to prepare, but there are many types of component preparation work, and it is inevitable that one person will take multiple jobs, so that too much plasma may melt and cryoprecipitate coagulation factors may flow into empty bags.
But for a long time, there has not been such a method in this field, and it has become a technical problem that this field has always been eager to solve but has never been successful.

Method used

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  • Method for preparing cryoprecipitate coagulation factor
  • Method for preparing cryoprecipitate coagulation factor

Examples

Experimental program
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Effect test

Embodiment

[0078] 1 Materials and methods

[0079] 1.1 Instruments, materials and reagents

[0080] 1.1.1 Instruments and materials:

[0081] Microwave oven (Panasonic-NN-CS957S);

[0082] DLM12L ultra-large capacity refrigerated centrifuge (Changsha Xiangzhi);

[0083] Water-bath low-temperature melter (Dakewei Biotechnology Co., Ltd.);

[0084] Refrigerator (Haier Company);

[0085] High-efficiency heat sealing machine (Shenzhen Dakowei Medical Technology Co., Ltd.);

[0086] Electronic scale (Shanghai Jingtian Electronic Instrument Co., Ltd.);

[0087] BE-THROMBOTIMER4 blood coagulation instrument (German BE company);

[0088] The 400ml five-pack preservation solution is CPDA-1 anticoagulant (Shandong Weigao).

[0089] 1.1.2 Reagents:

[0090] IBS buffer, lot number 036N-D064A; FactorVIII, lot number 11237812; APTT-EA, lot number 109130410; CaCl 2 , batch number 11108071; fibrinogen assay kit, batch number 11129124; a full set of reagents are provided by German Metron.

[00...

Embodiment 1

[0104] (3) Embodiment 1: microwave oven melting centrifugation (the present invention):

[0105] Weigh 10 copies of FFP in groups of 5, turn on the microwave oven, spread 5 bags of fresh frozen plasma in the microwave box, select the temperature control function, set the temperature: 5°C, select the steam thawing program, enter Weight, start thawing. After thawing, the ice-slag-like mixture was placed in a refrigerated centrifuge at a temperature of 2°C±2°C and a centrifugal force of 3800g, and centrifuged for 10min. Separate the upper layer of plasma, and the rest of 20-30ml is the cryoprecipitate.

[0106] 1.2.3. Detection method of factor VIII and fibrinogen:

[0107] Strictly follow the operating instructions of the hemagglutination analyzer produced by BE Company and the instructions of German Metron reagents.

[0108] 1.2.4 Capacity:

[0109] Weigh with an electronic scale.

[0110] 1.2.5 Quality standard:

[0111] Cryoprecipitate from 200ml whole blood: Factor VII...

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Abstract

The invention provides a method for preparing a cryoprecipitate coagulation factor. The method for preparing the cryoprecipitate coagulation factor, provided by the invention, comprises the following steps: a) providing fresh lyophilized plasma; b) unfreezing: unfreezing the fresh lyophilized plasma through microwaves by adopting a frequency conversion microwave heating device to unfreezing temperature, so as to obtain an ice-block-shaped mixture; c) separating: separating a precipitated substance, which is not dissolved coldly, from the ice-block-shaped mixture, so as to obtain the cryoprecipitate coagulation factor. The method provided by the invention has the advantages of simple equipment and convenience for operation, short preparation time and accurate preparation capacity, more content of obtained VIII factors, good clinical transfusion effect and the like.

Description

technical field [0001] The invention relates to the field of blood component preparation, in particular to a method for preparing cryoprecipitated blood coagulation factor, in particular to a method for preparing cryoprecipitated blood coagulation factor by temperature-controlled microwaves. Background technique [0002] Cryoprecipitate coagulation factor ("cryoprecipitate" for short) refers to the fresh frozen plasma (FFP) during the storage period, which is isolated under sterile conditions at 1°C to 6°C after melting in a closed state at 1°C to 6°C. Blood components made from cold insoluble substances precipitated in plasma and frozen within 1 hour (National Standard of the People's Republic of China: Quality Requirements for Whole Blood and Blood Components [S].GB18469-2012: 5.18). [0003] Cryoprecipitate is a blood component commonly used clinically because of its small preparation capacity, high concentration and purity, and good therapeutic effect. It mainly contain...

Claims

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Application Information

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IPC IPC(8): A61K35/16A61P7/04A61P7/00
CPCA61K9/0019A61K35/16
Inventor 侯广安孔卫建张媛马振东张福敏高占海侯维清
Owner 侯广安
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