Functional nucleic acid protective carrier based on dna hydrogel and its preparation method and application
A functional, hydrogel technology, applied in the field of biomedicine, to achieve the effects of slowing down degradation, good stability, and slowing down degradation
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Embodiment 1
[0047] The preparation route of the DNA hydrogel A of the present embodiment 1 is as follows: figure 1 As shown, the specific steps are as follows:
[0048] 1.1 Synthesis of Polymer 1
[0049] Take 2-chloro-caprolactone (700.0 mg) and dissolve it in 15 ml of anhydrous toluene, and remove the moisture in the solution by azeotroping toluene and water. ethanol (17 mg) and tin (II) octoate, after reacting for 20 minutes, the temperature rose to 130 ° C to continue the reaction for 4 hours, after the reaction was completed, repeated washing and purification 3 times under the conditions of dichloromethane / glacial ether, to obtain White powder (500 mg), that is, the degradable macromolecule-polymer 1 whose side chain is modified with chlorine element is obtained, and the yield is 69.7%.
[0050] Polymer 1 1 H NMR spectrum, such as figure 2 As shown, the test solvent is CDCl 3 , the assignment of each proton peak is as follows: δ (ppm): 4.32-4.27 (m, 64H, ClCH), 4.26-4.15 (m, 12...
Embodiment 2
[0081] The preparation route of the DNA hydrogel B of the present embodiment 2 is as follows Figure 16 As shown, the specific steps are as follows:
[0082] 2.1 Preparation of Polymer 4
[0083] In the glove box, compound 1 (700.0 mg) was dissolved in 20 ml of anhydrous N,N'-dimethylformamide, and 400 μl of newly prepared Ni(COD) depe was quickly added, and the solution changed from light yellow to Turn into bright yellow, and react at room temperature for 12 hours. After the reaction is completed, N,N'-dimethylformamide is distilled off under reduced pressure, and then washed and purified three times under the condition of dichloromethane / glacial ether, and vacuum-dried at room temperature to obtain White powder (600 mg), polymer 4, yield 85.7%.
[0084] 2.2 Preparation of Polymer 5
[0085] Dissolve polymer 4 (0.13 mg) in 500 microliters of dimethyl sulfoxide, add DBCO-DNA (2.3 mg), and react at 50°C for 24 hours. The ultrafiltration centrifuge tube was centrifuged to r...
Embodiment 3
[0106] The preparation route of the DNA hydrogel C of the present embodiment 3 is as follows Figure 17 As shown, the specific steps are as follows:
[0107] 3.1 Preparation of Polymer 6
[0108] Polyethylene glycol monomethyl ether (100.0 mg) was dissolved in 15 ml of anhydrous toluene, the trace moisture in the solution was removed by azeotroping of toluene and water, and the remaining toluene was removed under reduced pressure. Transfer to the glove box, add 10 ml of dried tetrahydrofuran, then add compound 2 (357 mg) and a drop of tin (II) octoate in sequence, and react at 35°C for 3 hours. Repeated elution and purification for 3 times, a white powder (232 mg) was obtained with a yield of 50.8%.
[0109] 3.2 Preparation of Polymer 7
[0110] Polymer 6 (0.110 mg) was dissolved in 500 microliters of dimethyl sulfoxide, added DBCO-DNA (2.8 mg), and reacted at 50 ° C for 24 hours. The centrifuge tube was centrifuged to remove unreacted DBCO-DNA, and the resulting solution ...
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