Pretreatment method for improving straw cellulose saccharification rate

A technology of straw cellulose and cellulose, which is applied in the field of enzymatic hydrolysis and saccharification treatment, can solve the problem of high consumption cost and achieve the effects of eliminating biological resistance, simple process and simple operation.

Inactive Publication Date: 2017-10-27
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But the consumption cost is high and has application limitations

Method used

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  • Pretreatment method for improving straw cellulose saccharification rate
  • Pretreatment method for improving straw cellulose saccharification rate

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Prepare sodium hydroxide with a mass fraction of 4%, 6% urea and 5% thiourea and dissolve them in deionized water to obtain a sodium hydroxide-urea-thiourea mixture; pre-cool the mixture to -10°C to Dissolve wheat straw caustic soda pulp to prepare a straw cellulose solution with a mass fraction of 3%. The resulting solution is diluted with water to precipitate 80% of the dissolved cellulose, centrifuged twice at 3000rpm / min to separate the cellulose, and washed with water until the pH value is 7.2. After air-drying, light yellow, fibrous regenerated cellulose is obtained; the regenerated cellulose is added to the acetic acid-sodium acetate buffer solution with a pH value of 4.5, until the mass concentration is 10%, and then cellulase is added, and the enzyme dosage is 10 FPU / g, Enzymolysis at 30°C for 10 hours to obtain a cellulose enzymatic solution containing a large amount of reducing sugar, and the saccharification rate of cellulose was measured to be 68.5%;

[00...

Embodiment 2

[0031]Dissolve 8% sodium hydroxide, 10% urea and 10% thiourea in deionized water to obtain a sodium hydroxide-urea-thiourea mixture; pre-cool the mixture to -20 °C to dissolve wheat straw Using caustic soda pulp, a straw cellulose solution with a mass fraction of 5% was obtained; diluted with water, 90% of the dissolved cellulose was precipitated, the cellulose was centrifuged at 4000rpm / min, washed with water to a pH value of 7.0, and light yellow was obtained after drying in an oven , fibrous regenerated cellulose; the regenerated cellulose is added to the acetic acid-sodium acetate buffer solution with a pH value of 5.5 to a mass concentration of 3%, then cellulase is added, and the enzyme dosage is 30 FPU / g, at 40°C After 20 hours of enzymatic hydrolysis, a cellulose enzymatic solution containing a large amount of reducing sugar was obtained, and the saccharification rate of cellulose was measured to be 94.1%;

[0032] Set up a control sample, use the same cellulase dosage...

Embodiment 3

[0034] Take 7% sodium hydroxide, 8% urea and 8% thiourea and dissolve them in deionized water to obtain a sodium hydroxide-urea-thiourea mixture; pre-cool the mixture to -12°C to dissolve the wheat straw A straw cellulose solution with a mass fraction of 5% was prepared from caustic soda pulp; diluted with water, 90% of the dissolved cellulose was precipitated, centrifuged at 5000rpm / min to separate the cellulose, washed with water to a pH value of 7.1, air-dried and then oven-dried to obtain Light yellow, fibrous regenerated cellulose; add regenerated cellulose to citric acid-sodium citrate buffer solution with a pH value of 6.0 to a mass concentration of 5%, then add cellulase, and the enzyme dosage is 35 FPU / g , enzymatically hydrolyzed at 50°C for 15 hours to obtain a cellulose hydrolyzate containing a large amount of reducing sugar, and the saccharification rate of cellulose was 92.4%;

[0035] Set up a control sample, use the same cellulase dosage of 35FPU / g, the same ce...

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Abstract

The invention discloses a pretreatment method for improving the straw cellulose saccharification rate, and relates to the field of enzymatic saccharification treatment. Common waste straws are selected as raw materials; the raw materials are rich, and the yield is enough and is guaranteed; the wastes are reutilized; the raw material cost is low; the energy is saved; the consumption is reduced; alkali-urea-thiourea can also be recovered and utilized. The pretreatment method has the advantages that the process is simple; through the dissolution, regeneration and enzymolysis processes, the natural crystal structure of cellulose is changed; the bio-resistance on the cellulase is eliminated; the wood cellulose enzymatic saccharification is realized by a method with the advantages that the operation is simple, the time and the labor are saved, high efficiency is realized, and the industrialization is easy; the cellulose is fast changed into reducing sugar through enzymolysis; the enzymolysis efficiency is improved; important practical significance is realized on cellulose energy regeneration and resource utilization.

Description

technical field [0001] The invention discloses a pretreatment method, which relates to the field of enzymatic hydrolysis and saccharification treatment, in particular to a pretreatment method for improving the saccharification rate of straw cellulose. Background technique [0002] Chinese farmers have a long history of using crop stalks. Due to the low level of agricultural production and low output in the past, the amount of stalks was small. Apart from a small amount of stalks used for gaskets, feeding livestock, and some used for composting, most of them were burned as fuel. Lost. With the development of agricultural production, since the 1980s, China's grain output has increased significantly, and the amount of straw has also increased. In addition, the promotion of firewood-saving and coal-saving technologies, the popularization of coal burning and the use of liquefied petroleum gas have resulted in a large amount of surplus straw in rural areas. . The comprehensive u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02
CPCC12P19/02C12P19/14C12P2201/00Y02P20/10
Inventor 陈洪雷刘玉孔凡功赵鑫王守娟
Owner QILU UNIV OF TECH
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