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Vaccine composition, kit and application

A technology of vaccine composition and kit, applied in the direction of vaccines, multivalent vaccines, veterinary vaccines, etc., can solve the problems of CDV virus differences, harm, loss, etc., and achieve good prevention and control effects

Pending Publication Date: 2017-11-07
PU LIKE BIO ENG
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The currently used canine vaccine vaccines are immunized individually or in combination according to different ages of dogs and different immunization procedures, but the canine distemper virus antigens in these vaccines are the strains Onderstepoort or Synder that were popular in North America in the 1930s and 1940s. The Hill strain is very different from the popular CDV virus, and there is a possibility of immune evasion, especially in the clinical cases of these imported vaccines since they were introduced into China, the single or mixed infection of canine distemper has not been significantly suppressed or reduced. It still causes great harm and serious losses to the breeding industries such as pet dogs, dog breeding and fur economic animals

Method used

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  • Vaccine composition, kit and application
  • Vaccine composition, kit and application
  • Vaccine composition, kit and application

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Experimental program
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Embodiment approach

[0026] As an embodiment of the present invention, the content of the inactivated whole virus antigen of the canine distemper virus is 10% before inactivation. 3 -10 9 TCID 50 / ml.

[0027] The term "TCID 50 ” refers to the tissue culture infectious dose, and it is defined as the dilution of virus required to infect 50% of a given batch of inoculated cell cultures. Various methods can be used to calculate the TCID 50, including the Sperman-Karber method, which is used throughout the specification. For a description of the Spearman-Karber method, see BW Mahy, HO Kangro. Virology methods manual, 1996:25-46. In the present invention, when the antigen content is the inactivated antigen content, it means the antigen content before inactivation.

[0028] As a preferred embodiment of the present invention, the content of the inactivated whole virus antigen of the canine distemper virus is 10% before inactivation. 4 -10 6 TCID 50 / ml. As an embodiment of the present invention,...

Embodiment 1

[0062] Example 1 Isolation, identification and content determination of canine distemper virus strain

[0063] 1.1 Isolation and identification of canine distemper virus strains

[0064] Canine distemper virus was isolated from the lung lesions of dogs suspected to have outbreaks of canine distemper by technicians of Pulaike Bioengineering Co., Ltd., and named it CDV C / HN / 001 strain.

[0065] Using CDV H-F: 5'TTAGGGCTCAGGTAGTCCA3', CDV H-R: 5'CTAAG KCCAATTGARATGTGT3' as primers, one-step RT-PCR was performed according to the instructions of the EasyScript One-Step RT-PCR SuperMix (purchased from Beijing Quanshijin Biotechnology Co., Ltd.) Technology to amplify the H gene sequence of CDV C / HN / 001 strain, amplify the target fragment and connect it to the T vector and transform it. Select the positive cloned plasmid and send it to Yingwei Jieji (Shanghai) Trading Co., Ltd. for sequencing. The result is shown in SEQID No.1 , consistent with about 1800bp of the nucleic acid electr...

Embodiment 2

[0069] The preparation of embodiment 2 vaccine composition

[0070] 2.1 Preparation of CDV inactivated whole virus antigen

[0071] The CDV C / HN / 001 strain is inactivated through a final concentration of 0.025% V / V BPL (β-propiolactone), and according to "Chinese Veterinary Pharmacopoeia" (Chinese Veterinary Drug Committee, 2010 edition three, China Agricultural Press , 2010) carried out sterility test, mycoplasma test and exogenous virus test on CDV C / HN / 001, and the results showed that after inactivation of CDV C / HN / 001 strain, it was not polluted by bacteria and mold, nor was it polluted by bacteria or mold Mycoplasma and exogenous virus infection, good purity.

[0072] 2.2 Preparation of CDV subunit antigen

[0073] According to the H protein and F protein gene sequences (see SEQ ID No.1, SEQ ID No.3) of the CDV C / HN / 001 strain measured according to Example 1, and the M protein gene sequence of the CDV SY strain (see NCBI Accession number: KJ466106), respectively design...

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Abstract

The invention relates to a vaccine composition containing an immunizing amount of canine distemper virus antigens and other antigens, a preparation method and application, and relates to a preparation method and application of a kit containing an immunizing amount of canine distemper virus antigens. The prepared vaccine composition and kit can be efficiently applied to medicines for preventing and / or treating diseases related to canine distemper. The vaccine composition and kit can efficiently prevent and treat infection with popular canine distemper virus strains at the present, and can be efficiently immune to multiple pathogens.

Description

technical field [0001] The invention belongs to the field of veterinary biological products, and in particular relates to a vaccine composition containing canine distemper virus antigens and other common canine disease virus antigens, a kit and a preparation method and application thereof. Background technique [0002] Canine distemper virus (CDV) is a viral disease with high morbidity and mortality worldwide. With the change of ecological environment and the evolution of animals and viruses, its host has been changed from the traditional canine Family (including dogs, foxes, raccoon dogs, etc.), Procyonidae and Mustelidae (mink, etc.) animals have expanded to all 8 families of Carnivora and many animals such as Artiodactyla Suidae, Primate Rhesus and Pinniped Sealidae, etc. And showing a growing trend. [0003] Canine parvovirus (Canine Parvovirus, CPV) causes acute onset, short course of disease, high mortality, and strong infectivity. It can infect dogs, foxes, minks, ra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/175A61K39/12A61K39/02A61P31/14C12N7/00C07K14/13
CPCA61K39/02A61K39/12A61K2039/5252A61K2039/552A61K2039/70C07K14/005C12N7/00C12N2760/18421C12N2760/18434A61K2300/00
Inventor 田克恭乔波孙进忠张许科
Owner PU LIKE BIO ENG
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