A microdroplet for cell-free protein synthesis and its preparation method

Abstract

The invention discloses micro-droplets for cell-free protein synthesis and a preparation method thereof. The method includes the steps that firstly, a micro-fluidic chip device is used; 2, raw materials for protein synthesis are mixed to serve as a water phase, and the water phase is injected from a water phase inlet 6; an oil phase is injected from an oil phase inlet 4; in a cross channel 13, the water phase disperses under the shear force of the oil phase to form water-in-oil micro-droplets, and the micro-droplets are collected from a micro-droplet outlet 7 and then put into a thermostatic reaction vessel. According to the method, the micro-droplets can be rapidly prepared through the micro-fluidic chip device, and the obtained micro-droplets are uniform in size and stable in structure, and can provide a site for in-vitro protein synthesis; due to the unique microscale of the micro-droplets, part of the micro-droplets are used for simulating a cell environment so that a protein synthesis system working in the cell environment originally can obtain a similar environment, which facilitates the work of the protein synthesis system. Protein synthesis can be quantified, and the micro-droplets can be applied to detection and diagnosis.

Description

technical field [0001] The invention relates to a micro-droplet for cell-free protein synthesis, a preparation method and application thereof. Background technique [0002] Since the 20th century, with the deconstruction of the structure and function of various biological macromolecules, life science has gradually cracked the secrets of biological genetic variation, and fully turned to the molecular level to decipher the life process. At the end of the 20th century, with the development of a large number of genome sequencing projects, the genetic information of various organisms was deciphered, and the rise of molecular biology allowed the use of molecular operations to reconstruct genetic information, thereby obtaining various biological products that can meet human needs. [0003] Protein is the main bearer of life activities, all kinds of life activities require the participation of protein, and protein is the direct product of genetic information expression, so using cel...

AI Technical Summary

Problems solved by technology

However, the traditional biosynthesis method is limited by the organism itself. It is necessary to consider the culture and survival of cells, adjust various environmental factors, and separation and purification are also difficult. At present, high-throughput, big data, fast and efficient research has become a major goal of protein synthesis. The trend of research, the traditional method can no longer meet the research requirements, and the cell-free protein synthesis method is superior to the traditional method in these aspects, can bypass the cumbersome process of transformed cells expressed in vivo, not limited to the cell itself, can incorporate non- Natural and isotope-labeled amino acids, enabling the production of proteins that are insoluble or toxic in vivo, and in addition, facilitate proteome screening projects

[0005] However, the current research on cell-free protein synthesis systems is mostly focused on protein synthesis at the macro scale, trying to achieve long-term and high-efficiency protein synthesis by various means, but the operation is too complicated to reflect the simplicity and high efficiency of cell-free protein synthesis characteristics

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