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Anti-protein adsorption capillary column and preparation method thereof

An anti-protein adsorption, capillary column technology, applied in separation methods, chemical instruments and methods, solid adsorbent liquid separation, etc., can solve the problems of high toxicity in the production process, complex preparation process, low production efficiency, etc. Protein adsorption performance, simple and fast preparation process

Active Publication Date: 2019-11-08
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] For this reason, the technical problem to be solved by the present invention is that the existing preparation process is complex, the operating conditions are harsh, the production process is highly toxic, the production efficiency is low, the production cost is high, and the prepared anti-protein adsorption capillary electrophoresis covalently bonded coating column In order to solve the problem of poor quality, and provide an anti-protein adsorption capillary column and its preparation method, the process flow is simple, the production process is environmentally friendly, the production efficiency is high, the production cost is low, and the prepared anti-protein adsorption capillary electrophoresis covalently bonded coating Layer column is of good quality

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  • Anti-protein adsorption capillary column and preparation method thereof
  • Anti-protein adsorption capillary column and preparation method thereof

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preparation example Construction

[0029] The preparation method of the above-mentioned capillary column resistant to protein adsorption uses diazo resin and glucuronolactone to self-assemble layer by layer on the inner wall of the quartz capillary column through electrostatic interaction, and undergoes photocuring and cross-linking reaction through ultraviolet light, so that the original The hydrogen bonds are converted to covalent bonds, resulting in a stable covalently bonded coating on the inner wall of the quartz capillary. Specifically, the preparation method comprises the following steps:

[0030] S1, the concentration is 0.1-100mg·ml -1 Photosensitive diazo resin solution, water, concentration 0.1-100mg·ml -1 The glucuronolactone aqueous solution and water are slowly injected into the capillary successively; the mass ratio of the photosensitive diazo resin and the glucuronolactone is 1:(1-1.5);

[0031] The concentration of the above-mentioned diazo resin and glucuronolactone aqueous solution is 0.1mg...

Embodiment 1

[0043] This embodiment provides an anti-protein adsorption capillary column, the inner wall of the capillary is modified with an anti-protein adsorption covalent bonding coating, and the anti-protein adsorption covalent bonding coating is composed of photosensitive diazo resin and Glucuronolactone is formed by photocuring crosslinking reaction under ultraviolet light irradiation, and the covalently bonded coating of anti-protein adsorption includes a layer of photosensitive diazo resin and a layer of glucuronolactone, and the anti-protein The adsorbed covalently bonded coating is approximately 1.8 nm thick.

[0044] The preparation method of the above-mentioned anti-protein adsorption capillary column comprises the following steps:

[0045] S0, capillary internal activation pretreatment

[0046] successively with a concentration of 0.1mol·L -1 Sodium hydroxide solution, distilled water, the concentration is 0.1mol L -1 Flush the capillary with hydrochloric acid solution, di...

Embodiment 2

[0051] This embodiment provides an anti-protein adsorption capillary column, the inner wall of the capillary is modified with 7 layers of anti-protein adsorption covalently bonded coating, and the anti-protein adsorption covalently bonded coating is made of photosensitive diazo Resin and glucuronolactone are formed by photocuring and crosslinking reaction by ultraviolet light irradiation, the photosensitive diazo resin and the glucuronolactone layers are alternately arranged, and the covalent anti-protein adsorption layer of each layer is The bonding coating has a thickness of 1.1 to 1.8nm and is a covalent bonding coating that resists protein adsorption.

[0052] The preparation method of the above-mentioned anti-protein adsorption capillary column comprises the following steps:

[0053] S0, capillary internal activation pretreatment

[0054] successively with a concentration of 0.09mol·L -1 Sodium hydroxide solution, distilled water, the concentration is 0.11mol L -1 Flus...

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Abstract

The invention relates to a protein adsorption-resistant capillary column. The capillary inner wall is modified with a number of protein adsorption-resistant covalently bonded coatings. The protein adsorption-resistant covalently bonded coating is formed through a photo-curing cross-linking reaction of photosensitive diazoresin and glucuronolactone under UV light irradiation. Each protein adsorption-resistant covalently bonded coating has thickness of 1.1-1.8 nm. The coating can be prepared in a water phase through a self-assembling method. The preparation process is simple and fast. The chemical bonding of the coating is realized through the photo-curing cross-linking reaction of photosensitive diazoresin and glucuronolactone so that the quality problem such as capillary blocking is reduced. The preparation method is simple and environmentally friendly and realizes high production efficiency and a low production cost. The protein adsorption-resistant capillary electrophoresis covalently bonded coated column has good quality.

Description

technical field [0001] The invention relates to the technical field of capillary column separation, in particular to a protein adsorption-resistant capillary column and a preparation method of the capillary column. Background technique [0002] With the rapid development of capillary electrophoresis combined with mass spectrometry and laser-induced fluorescence detection, capillary electrophoresis technology has been widely used in life sciences, biomedical engineering, environmental protection, food inspection and other fields, especially in various proteins, RNA, etc. Separation and analysis of biological macromolecules. However, because the quartz glass capillary used in capillary electrophoresis is negatively charged in a wide pH range of the buffer solution, the analyzed samples, especially biomacromolecular samples, are seriously adsorbed on the inner wall of the capillary, which affects the separation performance of capillary electrophoresis. , Coating modification o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D15/20B01D15/10
CPCB01D15/10B01D15/20
Inventor 丛海林于冰王明红
Owner QINGDAO UNIV
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