Method for screening pacific oysters capable of increasing content of unsaturated fatty acid C20:3omega6 and related SNP (Single Nucleotide Polymorphism) primer pair

Abstract

The invention relates to an SNP (Single Nucleotide Polymorphism) marker for identifying pacific oyster individuals capable of increasing content of an unsaturated fatty acid epoxyeicosatrienoic acid (C20:3omega6) and development and application methods thereof. By virtue of early genome-wide association study, 38 clustered SNP signals in significant correlation with the content of C20:3omega6 located on chromosome 1 of the pacific oysters are identified. A corresponding detection method is developed aiming at one SNP site at the base scaffold 460_104,049 of the area, two base forms A / T exist at the site, and a nucleotide sequence of the upstream / downstream 500bp is shown as SEQ ID No.1. According to the detection method, AT genotype maternal oysters are screened to be used for guiding oyster breeding. The invention provides a method for performing genotype identification on the maternal oysters before fry breeding and increasing the content of the unsaturated fatty acid in the offspring. The result is an SNP marker obtained based on the genome-wide association study, and the effect is stable.

Description

technical field [0001] The invention belongs to the field of genetic engineering and genetic breeding, and relates to a pair of primers for screening SNPs related to the method for increasing the content of unsaturated fatty acid C20:3Ω6 in oysters. Background technique [0002] Oysters are an important marine aquatic resource and one of the most important marine cultured shellfish in the world. Although my country's oyster production is very high, the export volume only accounts for 3.3% of the world's oyster trade volume, and the average price is only 1 / 3 of other countries' oyster products, which shows that most oysters produced in China can only be produced and sold by themselves, and it is difficult to enter the international market. market and domestic high-end market. The main reason why Chinese oysters are hard to find in the international market is that the product specifications do not meet the quality standards of international trade. In fact, this is also the mai...

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Problems solved by technology

However, this method is based on prior information and develops polymorphic sites on known functional genes. The relative number of markers is small, and the main effect sites may not be obtained, and the genetic regulation mechanism of the phenotype cannot be determined. have a comprehensive understanding

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