A kind of denitrification and denitrification composite bacterial agent and its preparation method and application

A compound bacterial agent and denitrification technology, applied in the field of environmental bioengineering and water treatment, can solve the problems of increasing sewage treatment costs, unstable treatment effect, low denitrification rate, etc., to improve the degradation rate and COD removal rate, Ease of storage and transportation, high nitrate nitrogen degradation rate and high COD removal rate

Active Publication Date: 2021-06-01
河南中顺环保科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Biological denitrification technology is the use of microbial denitrification to remove pollutants such as nitrate nitrogen and nitrite nitrogen in wastewater. At present, there are problems such as low denitrification rate and poor treatment efficiency.
In order to solve this problem, the denitrification treatment effect is usually improved by reducing the treatment load, increasing the sewage treatment time, and improving the sludge settling property. However, the treatment effect of these measures is very unstable, and it will also increase the sewage treatment cost and increase economic pressure

Method used

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  • A kind of denitrification and denitrification composite bacterial agent and its preparation method and application
  • A kind of denitrification and denitrification composite bacterial agent and its preparation method and application

Examples

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Effect test

Embodiment 1

[0034] A denitrification and denitrification composite bacterial agent is prepared from the following bacterial strains in volume percentage: 20% of Paracoccus, 20% of Paleobacterium pallidus, and 60% of Achromobacter.

[0035] As mentioned, the number of live bacteria of Paracoccus is 1×10 7 -1×10 9 CFU / g; the number of viable bacteria of the pale bacillus is 1×10 7 -1×10 10 CFU / g; the number of viable bacteria of the achromobacter is 1×10 5 -1×10 9 CFU / g.

[0036] A preparation method of a denitrification and denitrification compound bacterial agent is prepared by the following steps:

[0037] 1) Take two rings each of Paracoccus, Paleobacterium, and Achromobacter that were preserved on the slant, and inoculate them in solid medium for cultivation at a temperature of 25°C for 3 days to obtain Paracoccus, Paleobacterium, and Achromobacter, respectively. Activated bacteria;

[0038] 2) Divide the enrichment medium into 250mL Erlenmeyer flasks, 50mL / bottle; take two ring...

Embodiment 2

[0052] A denitrification and denitrification composite bacteria agent is prepared from the following bacteria species in volume percentage: 25% of Paracoccus, 25% of Paleobacterium, and 50% of Achromobacter.

[0053] As mentioned, the number of live bacteria of Paracoccus is 1×10 7 -1×10 9 CFU / g; the number of viable bacteria of the pale bacillus is 1×10 7 -1×10 10 CFU / g; the number of viable bacteria of the achromobacter is 1×10 5 -1×10 9 CFU / g.

[0054] A preparation method of a denitrification and denitrification compound bacterial agent is prepared by the following steps:

[0055] 1) Take two rings each of Paracoccus, Paleobacterium, and Achromobacter that were preserved on the slant, and inoculate them in solid medium for culture, at a culture temperature of 30°C, and for 2 days, to obtain Paracoccus, Paleobacteria, and Achromobacter, respectively. Activated bacteria;

[0056] 2) Divide the enrichment medium into 250mL Erlenmeyer flasks, 70mL / bottle; take two rings...

Embodiment 3

[0070] A denitrification and denitrification composite bacterial agent is prepared from the following bacterial strains in volume percentage: 30% of paracoccus, 30% of paleobacterium, and 40% of achromobacter.

[0071] As mentioned, the number of live bacteria of Paracoccus is 1×10 7 -1×10 9 CFU / g; the number of viable bacteria of the pale bacillus is 1×10 7 -1×10 10 CFU / g; the number of viable bacteria of the achromobacter is 1×10 5 -1×10 9 CFU / g.

[0072] A preparation method of a denitrification and denitrification compound bacterial agent is prepared by the following steps:

[0073] 1) Take two rings each of Paracoccus, Paleobacterium, and Achromobacter that were preserved on the slant, and inoculate them in solid medium for culture. The culture temperature is 35°C, and the culture time is 1 day. Paracoccus, Paleobacterium, and Achromobacter are obtained respectively. Activated bacteria;

[0074] 2) Divide the enrichment medium into 250mL Erlenmeyer flasks, 100mL / bo...

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Abstract

The invention discloses a denitrification and denitrification composite bacterial agent, which is prepared from the following bacterial strains in volume percentage: 20-30% of Paracoccus, 20-30% of Paleobacterium pallidus, and 40-60% of Achromobacter. The invention also discloses the preparation method of the denitrification and denitrification composite bacterial agent and its application in the treatment of sewage containing nitrate nitrogen. The denitrification and denitrification compound bacterial agent of the present invention has the excellent performance of strong adaptability, short treatment time, high nitrate nitrogen degradation rate and COD removal rate through the synergistic effect of Paracoccus, Paleobacterium and Achromobacter, and is used in In sewage, the degradation rate of nitrate nitrogen and the removal rate of COD in sewage are significantly improved, the cost is greatly saved, and it has broad application prospects.

Description

technical field [0001] The invention belongs to the fields of environmental bioengineering and water treatment, and in particular relates to a denitrification and denitrification composite bacterial agent and a preparation method and application thereof. Background technique [0002] With the rapid development of China's economy, a large amount of pollutants such as nitrogen and phosphorus are discharged into water bodies, causing serious eutrophication of water bodies, which has become one of the reasons that threaten the living environment of human beings. Nitrogen pollution in water bodies in China is most serious in the form of nitrate nitrogen and nitrite nitrogen, and biological nitrogen removal is one of the most effective methods to remove nitrogen pollution in water bodies. [0003] Biological denitrification technology uses microbial denitrification to remove pollutants such as nitrate nitrogen and nitrite nitrogen in wastewater. At present, there are problems such...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C12R1/025C02F101/16
CPCC02F3/34C02F2101/163C12N1/20
Inventor 杨革刘胜格车程川巩志金刘金锋安笑迟
Owner 河南中顺环保科技有限公司
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