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Hydrogel for repairing injured tissue and preparation method thereof

A tissue injury and hydrogel technology, which is applied to medical preparations containing active ingredients, drug combinations, and pharmaceutical formulas, can solve problems such as PGE2 hydrogels that have not yet been developed, and achieve low prices, accelerated healing, and effective therapeutic effects Effect

Inactive Publication Date: 2017-12-22
TIANJIN AMCELLGENE ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports of hydrogels containing PGE2

Method used

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  • Hydrogel for repairing injured tissue and preparation method thereof
  • Hydrogel for repairing injured tissue and preparation method thereof
  • Hydrogel for repairing injured tissue and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for preparing a hydrogel for repairing tissue damage, comprising the steps of:

[0032] (1) dissolving chitosan with sterile distilled water, swelling at 6° C. for 18 hours to obtain a hydrogel with a mass content of chitosan of 1%;

[0033] (2) PGE2 powder is dissolved in neutral phosphate buffer to make the PGE2 solution that concentration is 1mg / ml, is the ratio of 1:2 by mass ratio, described PGE2 solution is added dropwise to step (1) obtained In the hydrogel, stirred for 4 hours at 6°C; stored at 6°C, the PGE2 is the abbreviation of prostaglandin E2.

[0034] This embodiment can also be stored at 2°C or 8°C.

Embodiment 2

[0036] A method for preparing a hydrogel for repairing tissue damage, comprising the steps of:

[0037] (1) Use sterile distilled water to dissolve the hydrogel material (collagen and hyaluronic acid with a mass ratio of 1:1), and swell at 2°C for 24 hours to obtain a hydrogel with a mass content of 0.01% of the hydrogel material;

[0038] (2) Dissolving the PGE2 powder in neutral phosphate buffer to make a PGE2 solution with a concentration of 0.1 mg / ml, and adding the PGE2 solution dropwise to step (1) in a mass ratio of 1:1 to obtain Stir in the hydrogel at 2°C for 5 hours; freeze at -18°C, and the PGE2 is the abbreviation of prostaglandin E2.

Embodiment 3

[0040] A method for preparing a hydrogel for repairing tissue damage, comprising the steps of:

[0041] (1) Use sterile distilled water to dissolve fibrin, and swell at 8°C for 12 hours to obtain a hydrogel with a fibrin content of 5%;

[0042] (2) PGE2 powder is dissolved in neutral phosphate buffer solution and is made the PGE2 solution that concentration is 5mg / ml, is the ratio of 1:4 by mass ratio, described PGE2 solution is added dropwise to step (1) obtained In the hydrogel, stir for 3 hours at 8°C; freeze at -22°C, and the PGE2 is the abbreviation of prostaglandin E2.

[0043] In the following content, the hydrogel used for repairing tissue damage is referred to as PGE2 hydrogel.

[0044] Experiment 1:

[0045] The characteristic of PGE2 hydrogel (experimental example 1 preparation)

[0046] Temperature Stability of PGE2 Hydrogels

[0047] In order to evaluate the influence of temperature on the stability of PGE2 hydrogel, the PGE2 hydrogel (prepared in Example 1) sto...

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Abstract

The invention discloses hydrogel for repairing injured tissue and a preparation method thereof. The preparation method comprises the following steps: dissolving a hydrogel material through sterile distilled water, and swelling to obtain hydrogel; dissolving PGE2 powder into a phosphate buffering solution to prepare a PGE2 solution; dropwise adding the PGE2 solution into the hydrogel; stirring for 3-5 hours under the temperature of 2-8 DEG C; storing or storing by freezing, wherein the PGE2 is the abbreviation of prostaglandin E2. With the adoption of the hydrogel, the release time of PGE2 can be certainly prolonged; the hydrogel is directly applicable to the treatment of tissue injury, and is capable of promoting the recovery of injured skin tissue and function; the hydrogel is capable of inducing macrophage at the injured site to polarize to reach M2 type so as to inhibit inflammatory, thereby accelerating the healing of the injured tissue; in addition, the blood vessel at the injured part is promoted to regrow in early stage. Additionally, the hydrogel is free from cytotoxicity, can directly coat the surface of the injured tissue, is convenient to use, and low in price, and has an effective treatment effect.

Description

technical field [0001] The invention relates to a hydrogel for repairing tissue damage and a preparation method thereof. Background technique [0002] Skin wound (Skin Wound) has become a serious health problem. Trauma, burns, chronic ulcers, etc. cause large areas of skin defects, often forming wounds that are difficult to heal, which not only causes pain to patients, but also reduces the quality of life. Current proven treatments, including stem cell therapy and skin grafts, suffer from high medical costs that limit their use. Prostaglandin E2 (PGE2) is an important cell growth and regulatory factor, its role is to dilate blood vessels, increase organ blood flow, and has immunosuppressive and anti-inflammatory effects. And it has been reported that mesenchymal stem cells (MSCs) can play a role in promoting skin healing by regulating local inflammatory response. The mechanism of action may be that PGE2 secreted by MSCs induces the polarization of macrophages to the M2 ty...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K31/5575A61K47/36A61K47/42A61P17/02A61P29/00
CPCA61K9/0002A61K9/0014A61K9/06A61K31/5575A61K47/36A61K47/42
Inventor 李宗金张帅强韩之波
Owner TIANJIN AMCELLGENE ENG
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