Human placenta lyophilized powder preparation and preparation method thereof
A technology of freeze-dried powder and human placenta, which is applied in freeze-dried transportation, powder transportation, digestive system, etc. It can solve the problems of difficulty in exerting the efficacy of active molecules and loss of efficacy of biologically active substances, so as to avoid the risk of microbial growth and fully extract Intact, less digestive loss effect
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Embodiment 1
[0023] Example 1 Preparation of human placenta freeze-dried powder preparation
[0024] Proceed as follows:
[0025] (1) Collect healthy placenta with natural delivery and gestational age less than 29 weeks. During the collection process, wash the placenta with 1000ml of 0.9% normal saline; disinfect the surface of the cleaned placenta with 75% alcohol, and then rinse with 500ml of normal saline Drain the cleaned and disinfected placenta and put it into a sterile sampling bag, and add 1000ml of PBS buffer solution to the sampling bag, and then seal the sampling bag; Transport to the preparation site within 22 hours at 20°C;
[0026] (2) Take out the placenta, rinse with 4°C normal saline and disinfect the surface of the placenta with alcohol, remove the fascia and blood vessels, put it in a NaCl solution with a mass concentration of 10%, soak it for 5 hours, and take it out;
[0027] (3) Shred the placenta (cut into about 3-5cm 3 small pieces) were subjected to low-temperat...
Embodiment 2
[0032] Example 2 Preparation of human placenta freeze-dried powder preparation
[0033] Proceed as follows:
[0034] (1) Collect healthy placenta with natural delivery and gestational age less than 29 weeks. During the collection process, wash the placenta with 1000ml of 0.9% normal saline; disinfect the surface of the cleaned placenta with 75% alcohol, and then rinse with 500ml of normal saline Drain the cleaned and disinfected placenta and put it into a sterile sampling bag, and add 1000ml of PBS buffer solution to the sampling bag, and then seal the sampling bag; Transport to the preparation site within 22 hours at 20°C;
[0035] (2) Take out the placenta, rinse with 4°C normal saline and disinfect the surface of the placenta with alcohol, remove the fascia and blood vessels, put it in a NaCl solution with a mass concentration of 10%, soak it for 5 hours, and take it out;
[0036] (3) Shred the placenta (cut into about 3-5cm 3 small pieces) were subjected to low-temperat...
Embodiment 3
[0041] Example 3 Preparation of human placenta freeze-dried powder preparation
[0042] Proceed as follows:
[0043] (1) Collect healthy placenta with natural delivery and gestational age less than 29 weeks. During the collection process, wash the placenta with 1000ml of 0.9% normal saline; disinfect the surface of the cleaned placenta with 75% alcohol, and then rinse with 500ml of normal saline Drain the cleaned and disinfected placenta and put it into a sterile sampling bag, and add 1000ml of PBS buffer solution to the sampling bag, and then seal the sampling bag; Transport to the preparation site within 22 hours at 20°C;
[0044] (2) Take out the placenta, rinse with 4°C normal saline and disinfect the surface of the placenta with alcohol, remove the fascia and blood vessels, put it in a NaCl solution with a mass concentration of 10%, soak it for 5 hours, and take it out;
[0045] (3) Shred the placenta (cut into about 3-5cm 3 small pieces) were subjected to low-temperat...
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