Unlock instant, AI-driven research and patent intelligence for your innovation.

The Method and Application of Establishing Fingerprint of Xinjiang Zicao Based on HPCE

A technology of Xinjiang comfrey and fingerprints, applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of cumbersome processing, poor separation effect of active ingredient shikonin, poor detection effect and separation effect, etc., and achieve sample processing Simple, improved surface activity, relatively stable effect of retention value

Active Publication Date: 2020-08-04
MINZU UNIVERSITY OF CHINA
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the further treatment of naphthoquinone components after the preparation of crude extract by this technology is relatively cumbersome, and the separation effect on shikonin, the active ingredient of comfrey, is also poor, and the detection effect and separation effect on other components of Xinjiang comfrey extract are not good. Well, at the same time, in order to reduce the interference of the solvent peak at the detection wavelength of 200nm, it is necessary to further explore better electrophoresis conditions

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • The Method and Application of Establishing Fingerprint of Xinjiang Zicao Based on HPCE
  • The Method and Application of Establishing Fingerprint of Xinjiang Zicao Based on HPCE
  • The Method and Application of Establishing Fingerprint of Xinjiang Zicao Based on HPCE

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] 1 Instruments and reagents

[0059] (1) Instrument

[0060] P / ACE MDQ capillary electrophoresis instrument (Beckman Coulter, USA), equipped with ultraviolet detector; uncoated fused silica capillary (inner diameter is 50 μm, total length is 50.2 cm, effective length is 40.0 cm, Hebei Yongnian Ruifeng Chromatography Device Co., Ltd. ); XP205 electronic analytical balance (Swiss METTLER TOLEDO company); pH meter (Sartorius PB-10); KQ-500B ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.).

[0061] (2) Reagents

[0062] Levoshikonin Shikonin: China Institute for the Control of Pharmaceutical and Biological Products, batch number: 110769-200405. Acetylshikonin: Shanghai Yuanye Biotechnology Co., Ltd., batch number: 24502-78-1. Boric acid (analytical grade, Sinopharm Chemical Reagent Co., Ltd.), borax (analytical grade, Beijing Chemical Plant), absolute ethanol (analytical grade, Beijing Chemical Plant), sodium dodecyl sulfate (SDS, Sigama) during other experim...

Embodiment 2 Embodiment 5

[0078] The difference between Embodiment 2 to Embodiment 5 and Embodiment 1 is that some electrophoresis conditions are different, and others are the same as Embodiment 1.

[0079] The electrophoresis condition of table 1 embodiment two-embodiment five

[0080]

[0081]

[0082] Result: The electrophoretic fingerprints of Examples 2 and 3 are similar to those of Example 1, and 6 characteristic peaks are obtained. The distance between each peak is similar to that of Example 1, and the degree of separation is better. Embodiment 4 obtains 6 characteristic peaks, but the degree of separation is slightly poor. Embodiment 5 also obtains 6 characteristic peaks, the distance between each peak is relatively large, and the degree of separation is better, but the required separation time is slightly longer.

Embodiment 6

[0083] Example 6 Application of Xinjiang Comfrey Capillary Electrophoresis Fingerprint Method

[0084] Medicinal material: Xinjiang Zicao Control medicinal material: Beijing Beina Chuanglian Biotechnology Research Institute, batch number: 160329. Inner Mongolia comfrey reference medicinal material: Beijing Beina Chuanglian Biotechnology Research Institute, batch number: 151208. The remaining six Xinjiang comfrey medicinal materials were purchased or produced from: Huocheng of Xinjiang, Anguo of Hebei, Aksu of Xinjiang, Yining of Xinjiang, Hehuachi of Chengdu, and Zhenxiong of Zhaotong of Yunnan.

[0085] The reagents, instruments, electrophoresis conditions, test solution and standard solution preparation methods of this embodiment are all the same as those in Example 1.

[0086]Capillary electrophoresis analysis was performed on eight kinds of comfrey from different origins or places of purchase, and the electrophoresis patterns were as follows: figure 2 As shown, from top...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
lengthaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for establishing an arnebia euchroma johnst fingerprint spectrum based on HPCE (High-Performance Capillary Electrophoresis) and application of the arnebia euchroma johnst fingerprint spectrum. The method comprises the following steps: preparing a test sample solution and a control solution of arnebia euchroma johnst, and performing capillary electrophoresis analysis, wherein an operation buffer solution in capillary electrophoresis analysis comprises 15-35mmol*L / <-1> lauryl sodium sulfate. By adopting the method for establishing the arnebia euchroma johnst fingerprint spectrum, pretreatment of samples is simple and convenient, the electrophoresis process is efficient, rapid, convenient and pollution-free, the obtained spectrum has rich characteristic peaks and is high in stability and controllability, bases can be provided for quality control on arnebia euchroma johnst, and an identification method for distinguishing arnebia euchroma johnst from arnebia guttata bunge and other types of lithospermum is provided.

Description

technical field [0001] The invention belongs to the field of fingerprints of traditional Chinese medicines, and in particular relates to a method and application for establishing a fingerprint of Xinjiang comfrey based on HPCE. Background technique [0002] Comfrey is a traditional Chinese medicinal material in my country. The comfrey recorded in the 2015 edition of the Pharmacopoeia of the People's Republic of China is the dried root of Arnebia euchroma (Royle) Johnst. or Arnebia guttata Bunge of Inner Mongolia. Comfrey is sweet, salty, cold in nature, guixin, liver meridian, has the functions of clearing heat and cooling blood, promoting blood circulation and detoxification, penetrating rashes and eliminating spots. , the treatment of water and fire burns, etc., is a commonly used medicine for clearing away heat and detoxification, cooling blood and stopping bleeding in Chinese medicine clinics. Pharmacological studies have shown that comfrey also has anti-bacterial, anti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/447
CPCG01N27/44704
Inventor 申刚义张蕾张爱芹王嫚
Owner MINZU UNIVERSITY OF CHINA