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Preparation method of biosensor for alpha 2,3 saliva acidification glycan detection

A biosensor and sialylation technology, applied in the field of electrochemical detection, can solve problems such as difficulty in generating stable signals, and achieve the effects of facilitating commercialization, improving sensitivity, and increasing conductivity

Active Publication Date: 2018-01-23
CHONGQING MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, when MB is used alone, it is difficult to generate a stable signal, which limits its application

Method used

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  • Preparation method of biosensor for alpha 2,3 saliva acidification glycan detection
  • Preparation method of biosensor for alpha 2,3 saliva acidification glycan detection
  • Preparation method of biosensor for alpha 2,3 saliva acidification glycan detection

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Embodiment 1

[0039] Step 1. First, disperse 2 mg of n-C60 in 1 mL of 0.1M HCl, then sonicate for 2 hours until n-C60 is uniformly dissolved in the solution. Next, add 2 mL of ultrapure water to the solution. Next, the mixture was stirred and heated to 100°C. After that, 150 mg of AA, 1.17 mg of K 2PtCl 4 and 1.66 mg of Na 2PdCl 4 were added to 1 mL of ultrapure water, respectively. After heating at 100°C for 2.5 hours, the product was cooled to room temperature. Then, the mixture was run at 8000rpm min -1 Centrifuge for 3 minutes and wash three times to remove remaining AA, free Pt and Pd nanoparticles. Finally, dissolve the precipitate in 1 mL of ultrapure water before use for the next step.

[0040] Step 2. First, MB (9.8 mM, 1 mL) was added to HCl (0.1 M, 600 μL) and DTAB (1.14 mM, 6 mL) solution, and stirred thoroughly for about 10 minutes. Subsequently, HAuCl 4 (4%, 200 μL) was added to the compound and stirring was continued at room temperature for about 6 hours. Compound at 80...

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Abstract

The invention discloses a novel sandwich-type biosensor based on 4-mercapto-phenylboronic acid (4-MPBA). Alpha 2,3 saliva acidification glycan is one of tumor markers for early diagnosis of cancer. Bythe aid of boron atoms matched with the 4-MPBA and Neu5Ac amide groups, a new molecular recognition system is built and used for detecting the alpha 2,3 saliva acidification glycan for the first time. An amination fullerene palladium-platinum alloy is used for signal amplification, and sophora japonica agglutinin is covalently immobilized onto Au-polymethylene blue serving as a signal probe and used for specifically recognizing the alpha 2,3 saliva acidification glycan. Under optimal experimental conditions, the biosensor displays the wide linear range of 10fg mL<-1>-100ng mL<-1> and the detection limit of 3fg mL<-1> (S / N=3). Besides, the method shows good repeatability and stability and can be used for clinical research.

Description

Technical field: [0001] The invention relates to a preparation method and application of an electrochemical sensor for the quantitative diagnosis of α2,3 sialylated glycans in clinical practice, especially a sandwich-type biological sensor prepared based on gold polymethylene blue-sophora lectin as a nano-beacon The sensor is used for detecting α2,3 sialylated glycan and belongs to the field of electrochemical detection. Background technique: [0002] Due to the nine-carbon structure of sialic acid, it attaches to cell surface glycoconjugates as terminal monosaccharides. The α2,3 bond between sialic acid and N-acetyllactosamine structure (Galβ1-4GlcNAc) is a Golgi-mediated process mediated by β-galactoside α2,3 sialyltransferase (ST3Gal-I). Variants of α2,3 sialylation are associated with a wide range of biological and pathological diseases, including the development of certain cancers. When apoptosis occurs in tumor tissue, α2,3 sialoglycans (α2,3-sial-Gs) are synthesized...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 于超何俊琳袁祺奇
Owner CHONGQING MEDICAL UNIVERSITY
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