The invention discloses a gas-permeable antibacterial electrode sheet and a preparation method thereof, comprising a reusable electrode sheet and a conductive film detachable from the electrode sheet. The conductive film consists of a hydrogel layer, a silver layer and a hydrocolloid layer from bottom to top Composition, the electrode sheet and the hydrocolloid layer are closely pasted; the raw materials of the hydrogel layer include: eugenol, boron trifluoride ether, triisopropylsilane, liposomes, multi-walled carbon nanotubes, polydiallyl di Methylammonium chloride, nanocellulose, polyacrylamide, ammonium persulfate, methacrylic anhydride, polyhexamethylene monoguanidine hydrochloride, octylphenol polyoxyethylene ether, Tween‑80, anhydrous tris Fluoroacetic acid or boron trifluoride. The electrode sheet uses a composite conductive hydrogel as a conductive film, which has good conductivity and good biological safety. Under the action of sweat, it still has self-adhesiveness, is breathable, sweat-absorbent, and has antibacterial properties; it can be slightly deformed according to the elasticity of human skin. , without affecting the stability of the electrical signal; the electrode sheet is easy to remove and can be reused.