Compounds, conjugates, kits as well as applications thereof in detecting testosterone or analogues thereof

A technology of compounds and conjugates, applied in the field of analysis, can solve the problems of testosterone derivatives to be developed, and achieve the effect of being easy to popularize, apply and obtain

Active Publication Date: 2018-02-02
SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, currently applicable testos

Method used

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  • Compounds, conjugates, kits as well as applications thereof in detecting testosterone or analogues thereof
  • Compounds, conjugates, kits as well as applications thereof in detecting testosterone or analogues thereof
  • Compounds, conjugates, kits as well as applications thereof in detecting testosterone or analogues thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] In this embodiment, the compound shown in formula (2) is synthesized according to the following process:

[0055]

[0056] The synthetic route is as follows:

[0057]

[0058] 1. Synthesis of compound 2:

[0059] Feed 3.05g (0.01mol) of compound 1, 2.8g (0.02mol) of anhydrous potassium carbonate and 0.5g of DMAP into a 100mL dry round bottom flask, add 50mL of DMF and stir to dissolve. Add 3.53 g (0.015 mol) of TBS-Cl (tri-n-butylchlorosilane), raise the temperature to 60° C., and react for 18 hours. Sampling was carried out regularly, and the sample was detected by thin-layer chromatography (the system was n-hexane / ethyl acetate=6 / 1, color developed by phosphomolybdic acid). When the raw materials disappeared, the heating was stopped. After returning to room temperature, the reaction solution was transferred to a 250 mL separatory funnel, 150 mL of ethyl acetate was added, washed 4 times with water, dried over anhydrous sodium sulfate, concentrated, and drained...

Embodiment 2

[0068] In this embodiment, the compound shown in formula (3) is synthesized according to the following process:

[0069]

[0070] The synthetic route is as follows:

[0071]

[0072] Compound shown in synthetic formula (3):

[0073] Feed 0.91g (3mmol) of compound 5 into a 50mL dry and cooled reaction flask, add 20mL of dichloromethane, stir to dissolve, add 0.2g of DMAP, 0.48g (4.8mmol) of succinic anhydride, and stir at room temperature overnight (16 hours). The reaction solution was transferred to a separatory funnel, washed with water four times, dried over anhydrous sodium sulfate, concentrated, and purified through a column to obtain 0.95 g of the compound represented by formula (3), with a reaction yield of 78.7%.

[0074] MS: positive ion peaks: 403.6, 425.6 (+Na peak); negative ion peaks: 401.5.

Embodiment 3

[0076] The compounds obtained in Examples 1 and 2 were labeled with alkaline phosphatase according to the following steps to obtain conjugates 1 and 2, respectively.

[0077] Add the compound to be labeled to Mes (2-(N-morpholine)ethanesulfonic acid) buffer solution (pH=4.5), the final concentration is 0.2mg / mL; add alkaline phosphatase (ALP), the final concentration is 0.2mg / mL, mix well; then add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDAC), the final concentration is 2mg / mL, mix well; add nitrogen hydroxysuccinyl Imine (NHS), with a final concentration of 2 mg / mL, mixed; reacted at 25°C for 2 hours; replaced by ultrafiltration three times with Tris buffer at pH = 7.5, purified to remove excess testosterone derivatives and cross-linking agents, to obtain the conjugate.

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Abstract

The present invention discloses compounds, conjugates, kits for testing testosterone or analogues thereof, and applications of the kits for detecting testosterone or analogues thereof in detecting testosterone or analogues thereof. The compounds have a structure as shown in the formula (1), wherein L represents a linking arm, and R1, R2, R3 and R4 are each independently hydrogen, hydroxyl, C1-3 alkyl group, C1-3 alkoxyl, C2-3 alkenyl or C2-3 alkynyl. The compounds in the invention are capable of detecting testosterone or analogues thereof accurately. (The structures of the compounds are as shown in the description.)

Description

technical field [0001] The present invention relates to the field of analysis. In particular, the present invention relates to compounds, conjugates, kits and their use in the detection of testosterone or analogs thereof. More specifically, the present invention relates to a compound, a conjugate, a kit for detecting testosterone or its analogues and the use of the kit for detecting testosterone or its analogues in detecting testosterone or its analogues. Background technique [0002] Testosterone analogs refer to substances that have a similar structure to testosterone, that is, have the following mother nucleus structural unit. [0003] [0004] At present, the principle of using the immunological method to detect testosterone or its analogs is: since there is no functional group on testosterone or its analogs that can be directly connected to the labeled molecule, it is necessary to introduce a structure that can be directly connected to the labeled molecule through a...

Claims

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Application Information

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IPC IPC(8): C07J1/00C12N9/08C12N9/16C12N9/50G01N33/74G01N33/64G01N33/535
CPCC07J1/0059C07J1/007C12N9/0065C12N9/16C12N9/50C12Y111/01007C12Y301/03001G01N33/535G01N33/64G01N33/743G01N2333/575
Inventor 何飞张裕平刘锐
Owner SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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